In vitro evaluation of antagonistic microorganisms for the control of die-back of neem causal agent Phomopsis azadirachtae

• Autorzy: Girish K , Bhat S.S. , Raveesha K.A.

Warianty tytułu

PL

Ocena in vitro mikroorganizmow antagonistycznych do zwalczania zgorzeli Azadirachta indica wywolanej przez Phomopsis azadirachtae

• Języki publikacji: EN

Abstrakty

EN

The die-back of neem caused by Phomopsis azadirachtae is a devastating disease in India reducing the life span and seed production of neem. Six isolates of antagonistic bacteria and fungi, Bacillus cereus (MTCC 430), B. subtilis (MTCC 619), Pseudomonas aeruginosa (MTCC 2581), P. oleovorans (MTCC 617), Trichoderma harzianum (MTCC 792) and T. viride (MTCC 800) were evaluated against P. azadirachtae under in vitro conditions. Culture filtrates of all these microorganisms were extracted using ethyl acetate, and the obtained fractions were tested for their antifungal activity against P. azadirachtae at different concentrations. Ethyl acetate extracts of B. subtilis and P. aeruginosa were highly effective and completely inhibited the growth of P. azadirachtae at 25 ppm concentration. Both these isolates may be considered as factors for the biological control of die-back of neem.

PL

Zgorzel Azadirachta indicato wywołana przez Phomopsis azadirachtae jest ważną chorobą w Indiach, ograniczającą okres żywotności i produkcję nasion A. indica. Sześć izolatów antagonistycznych mikroorganizmów takich jak: Bacillus cereus (MTCC 430), B. subtilis (MTCC 619), Pseudomonas aeruginosa (MTCC 2581), P. oleovorans (MTCC 617), Trichoderma harzianum (MTCC 792) i T. viride (MTCC 800), przetestowano in vitro przeciwko P. azadirachtae. Filtraty kultur tych sześciu mikroorganizmów ekstrahowano octanem etylu. Otrzymane frakcje octanu etylu testowano pod względem aktywności przeciwgrzybowej. Były one wysoce efektywne w stężeniu 25 ppm i całkowicie inhibitowały wzrost P. azadirachtae, gdy były użyte w tym stężeniu. Zarówno B. subtilus jak i P. aeruginosa mogą być rozważone jako czynniki biologicznego zwalczania zgorzeli A. indcia.

Słowa kluczowe

PL

antagonistic microorganism; in vitro evaluation; control; biological control; Bacillus subtilis; plant disease; tree; neem tree; Phomopsis azadirachtae; Pseudomonas aeruginosa

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2009
• Tom: 49
• Numer: 4
• Opis fizyczny: p.362-368,ref.

Twórcy

autor

Girish K

• University of Mysore, Mysore - 570 006, India

autor

Bhat S.S.

autor

Raveesha K.A.

Bibliografia

• Agrios G. N. 2004. Plant Pathology. 5th ed. Elsevier Academic Press, San Diego, USA.
• Anjaiah V., Cornelis P., Koedam N. 2003. Effect of genotype and root colonization in biological control of Fusarium wilts in pigeonpea and chickpea by Pseudomonas aeruginosa PNA1. Can. J. Microbiol. 49: 85-91.
• Audenaert K., Pattery T., Cornelis P., Hofte M. 2001. Induced resistance to Botrytis cineria by Pseudomonas aeruginosa: role of siderophore and pyocyanin. IOBC/WPRS Bull. 24: 37-42.
• Bolwerk A., Lagopodi A. L., Lugtenberg B. J. J., Bloemberg G. V. 2005. Visualization of interactions between a pathogenic and beneficial Fusarium strain during biocontrol of tomato foot and root rot. Mol. Plant-Microbe Interact. 18: 710-721.
• Brent K. J. 1995. Fungicide resistance in crop pathogens: How can it be managed? In: FRAC Monograph No. l. Global Crop Protection Federation, Brussels, 48 pp.
• Bunker R. N., Mathur K. 2001. Integration of biocontrol agents and fungicide for suppression of dry root rot of Capsicum frutescens.J. Mycol. Plant Pathol. 31: 330-334.
• Carter S. D., Hein J. F., Rehnberg G. L., Laskey J. W. 1984. Effects of benomyl on reproductive development of male rats. J. Toxicol. Environ. Health 13, p. 53.
• Cavaglieri L., Orlando J., Rodriguez M. I., Chulze S., Etcheverry M. 2005. Biocontrol of Bacillus subtilis against Fusarium verticillioides, in vitro and at the maize root level. Res. Microbiol. l56: 748-754.
• Chaurasia B., Pandey A., Palni L. M., Trivedi P., Kumar B., Colvin N. 2005. Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in vitro. Microbiol. Res. 160: 75-81.
• Cubeta M. A., Hartman G. L., Sinclair J. B. 1985. Interaction between Bacillus subtilis and fungi associated with soybean seeds. Plant Dis. 69: 506-509.
• Dalvi R. R., Whittaker M. B. 1995. Toxicological implications of the metabolism of benomyl in animals. J. Environ. Biol. 16: 333-338.
• Dhingra O. D., Sinclair J. B. 1995. Basic Plant Pathology Methods. 2nd ed. CRC Press, Boca Raton, Florida, USA, p. 272.
• Dowling D. N., O'Gara F. 1994. Metabolites of Pseudomonas involved in the biocontrol of plant disease. Trends Biotechnol. 12: 133-144.
• Elad Y. 2003. Biocontrol of foliar pathogens: mechanisms and application. Commun. Agric. Appl. Biol. Sci. 68: 17-24.
• Fravel D. R. 1988. Role of antibiosis in the biocontrol of plant diseases. Annu. Rev. Phytopathol. 26: 75-91.
• Fuchs J., Defago G. 1991. Protection of cucumber plants against black root rot caused by Phomopsis sclerotioides with rhizobacteria. p. 57-62. In: "Plant Growth-Promoting Rhizobacteria - Progress and Prospects" (B. Keel C. Koller G. Defago, eds.). IOBC/WPRS Bull. XIV/8.
• Girish K., Shankara Bhat S., Raveesha K. A. 2009. In vitro screening of systemic fungicides against Phomopsis azadirachtae, the incitant of die-back disease of neem. Archiv. Phytopathol. Plant Protect. 42: 256-264.
• Hajra K. K., Khatua D. C., Mukherjee N. 1992. Antagonistic bacteria against fungal pathogens. J. Mycopathol. Res. 30: 65-70.
• Huang C. J., Wang T. K., Chung S. C., Chen C. Y. 2005. Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28-9. J. Biochem. Mol. Biol. 38: 82-88.
• Kita N., Ohya T., Uekusa H., Nomura K., Manago M., Shoda M. 2005. Biological control of damping-off of tomato seedlings and cucumber Phomopsis root rot by Bacillus subtilis RB14-C. JARQ 39: 109-114.
• Krishna Kishore G., Pande S., Podile A. R. 2005. Management of late leaf spot of groundnut (Arachis hypogaea) with chlorothalonil-tolerant isolates of Pseudomonas aeruginosa.Plant Pathol. 54: 401-408.
• Lange L., Breinholt J., Rusmussen F. W., Neilson R. I. 1993. Microbial fungicides - The natural choice. Pestic. Sci. 39: 55-160.
• Lavermicocca P., Valerio F., Evidente A., Lazzaroni S., Corsetti A., Gobbetti M. 2000. Purification and characterization of novel antifungal compounds from the sourdough Lactobacillus plantarum strain 21B. Appl. Environ. Microbiol. 66: 4084-4090.
• Leclere V., Bechet M., Adam A., Guez J. S., Wathelet B., Ongena M., Thonart P., Gancel F., Chollet-Imbert M., Jacques P. 2005. Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism's antagonistic and biocontrol activities. Appl. Environ. Microbiol. 71: 4577-4584.
• Lugtenberg B. J. J., Bloemberg G. V. 2004. Life in the rhizosphere. p. 403-430. In: "Pseudomonas", Vol. 1 (J. L. Ramos, ed.). Kluwer Academic/Plenum Publishers, New York.
• Maurhofer M., Keel C., Schnider U., Voisard C., Haas D., Defago G. 1992. Influence of enhanced antibiotic production in Pseudomonas fluorescens strain CHA0 on its disease suppressive capacity. Phytopathology 82: 190-195.
• Microbial Type Culture Collection. 2000. Bacteria - 2581: Pseudomonas aeruginosa. p. 37. In: "Catalogue of Strains". Institute of Microbial Technology, Chandigarh, India.
• Sateesh M. K. 1998. Microbiological investigations on die-back disease of neem (Azadirachta indica A. Juss.). Ph.D. Thesis. University of Mysore, India, 173 pp.
• Sateesh M. K., Shankara Bhat S., Devaki N. S. 1997. Phomopsis azadirachtae sp. nov. from India. Mycotaxon 65: 517-520.
• Shankara Bhat S., Sateesh M. K., Devaki N. S. 1998. A new destructive disease of neem (Azadirachta indica) incited by Phomopsis azadirachtae.Curr. Sci. 74: 17-19.
• Sharifi-Tehrani A., Shakiba M., Okhovat M., Zakeri Z. 2005. Biological control of Tiarosporella phaseolina the causal agent of charcoal rot of soybean. Commun. Agric. Appl. Biol. Sci. 70: 189-192.
• Siddiqui I. A., Ehteshamul-Haque S. 2001. Suppression of the root rot-root knot disease complex by Pseudomonas aeruginosa in tomato: The influence of inoculum density, nematode populations, moisture and other plant-associated bacteria. Plant Soil 237: 81-89.
• Singh D. P., Maurya S., Prakash O. M., Singh U. P. 2005. Phenolic composition and antifungal activity of culture filtrate of Leptoxyphium axillatum.Indian Phytopath. 58: 143-148.
• Sunish Kumar R., Ayyadurai N., Pandiaraja P., Reddy A. V., Venkateswarlu Y., Prakash O., Sakthivel N. 2005. Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosa PUPa3 that exhibits broadspectrum antifungal activity and biofertilizing traits. J. Appl. Microbiol. 98: 145-154.
• Thinggaard K. 1988. Biological control of root pathogenic fungi by Trichoderma. In: Symposium on Horticultural Substrates and their Analysis (J. Willumsen, ed.). ISHS Acta Horticulturae 221, Gl. Avernaes, Funen, p. 212.
• Wani A. H. 2005. Biological control of wilt of brinjal caused by Fusarium oxysporum with some fungal antagonists. Indian Phytopath. 58: 228-231.
• Zhang W., Watson A. K. 2000. Isolation and partial characterization of phytotoxins produced by Exserohilum monoceras, a potential bioherbicide for control of Echinochloa species. p. 125-130. In: Proc. of the X International Symposium on Biological Control of Weeds (N. R. Spencer, ed.). Montana State University, Bozeman, Montana, USA.