Protein profiling of sickle cell versus control RBC core membrane skeletons by ICAT technology and tandem mass spectrometry

• Autorzy: Chou J , Choudhary P.K. , Goodman S.R.
• Języki publikacji: EN

Abstrakty

EN

A proteomic approach using a cleavable ICAT reagent and nano-LC ESI tandem mass spectrometry was used to perform protein profiling of core RBC membrane skeleton proteins between sickle cell patients (SS) and controls (AA), and determine the efficacy of this technology. The data was validated through Peptide/Protein Prophet and protein ratios were calculated through ASAPratio. Through an ANOVA test, it was determined that there is no significant difference in the mean ratios from control populations (AA1/AA2) and sickle cell versus control populations (AA/SS). The mean ratios were not significantly different from 1.0 in either comparison for the core skeleton proteins (α spectrin, β spectrin, band 4.1 and actin). On the natural-log scale, the variation (standard deviation) of the method was determined to be 14.1% and the variation contributed by the samples was 13.8% which together give a total variation of 19.7% in the ratios.

Słowa kluczowe

EN

ion trap mass spectrometry; membrane skeleton; mass determination; human cell; erythrocyte; sickle-cell disease; proteomics

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2006
• Tom: 11
• Numer: 3
• Opis fizyczny: p.326-337,fig.,ref.

Twórcy

autor

Chou J

• University of Texas at Dallas, Richardson, Texas, USA

autor

Choudhary P.K.

autor

Goodman S.R.

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