DFF40-CAD hypersensitive sites are potentially involved in high molecular weight DNA fragmentation during apoptosis

• Autorzy: Widlak P.
• Języki publikacji: EN

Abstrakty

EN

Sequential cleavage of genomic DNA into large-scale DNA fragments of 50-300-kb, followed by formation of mono- and oligonucleosomal DNA fragments, is a biochemical hallmark of programmed, cell death (apoptosis). The endonuclease DFF40/CAD mediates regulated internucleosomal DNA fragmentation and chromatin condensation in cells undergoing apoptosis. DFF40 hypersensitive sites were detected in purified HeLa cell nuclei, and excision of 50-kb DNA fragments preceded formation of oligonucleosomal DNA ladders in nuclei treated with the nuclease. Topoisomerase II, but not topoisomerase I, stimulates DFF40 activity on plasmid DNA substrates. This suggests that interactions of DFF with the nuclear matrix-bound topoisomerase II may be involved in formation of DFF40 hypersensitive sites.

Słowa kluczowe

EN

chromatin; cell; HeLa cell; high molecular weight; apoptosis; topoisomerase II; endonuclease; DNA fragmentation

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2000
• Tom: 05
• Numer: 3
• Opis fizyczny: p.373-379,fig.

Twórcy

autor

Widlak P.

• Center of Oncology, 44-100 Gliwice, Poland

Bibliografia

• 1. Arends, M. J., Morris, R. G. and Wyllie, A. H. Apoptosis. The role of the endonuclease. Am. J. Pathol. 136 (1990) 593-608.
• 2. Zhivotovsky, B., Wade, D., Nicotera, P. and Orrenius, S. Role of nucleases in apoptosis. Int. Arch. Allergy Immunol. 105 (1994) 333-338.
• 3. Oberhammer, F., Wilson, J. W., Dive, C., Morris, I. D., Hickman, J. A., Wakeling, A. E., Walker, P. R. and Sikorska, M. Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of intemucleosomal fragmentation. EMBO J. 12 (1993) 3679-3684.
• 4. Filipski, J., Leblanc, J., Youdale, T., Sikorska, M. and Walker, P. R. Periodicity of DNA folding in higher order chromatin structures. EMBO J. 9 (1990) 1319-1327.
• 5. Lagarkova, M. A., Iarovaia, O. V. and Razin, S. V. Large-scale fragmentation of mammalian DNA in the course of apoptosis proceeds via excision of chromosomal DNA loops and their oligomers. J. Biol. Chem. 270 (1995) 20239-20241.
• 6. Walker, P. R., Weaver, V. M., Lach, B., LeBlanc, J. and Sikorska, M. Endonuclease activities associated with high molecular weight and internucleosomal DNA fragmentation in apoptosis. Exp. Cell Res. 213 (1994) 100-106.
• 7. Huang, P., Ballal, K. and Plunkett, W. Biochemical characterization of the protein activity responsible for high molecular weight DNA fragmentation during drug-induced apoptosis. Cancer Res. 57 (1997) 3407-3414.
• 8. Li, T.-K., Chen, A. Y., Yu, C., Mao, Y., Wang, H. and Liu, L. F. Activation of topoisomerase II - mediated excision of chromosomal DNA loops during oxidative stress. Genes Dev. 13 (1999) 1553-1560.
• 9. Liu, X., Zou, H, Slaughter, C. and Wang, X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 89 (1997) 175-184.
• 10. Enari, M., Sakahira, H., Yokoyama, H., Okawa, K., Iwamatsu, A. and Nagata, S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 391 (1998) 43-50.
• 11. Liu, X., Li, P., Widlak, P., Zou, H., Luo, X., Garrard, W. T. and Wang, X. DFF40 induces DNA fragmentation and chromatin condensation during apoptosis. Proc. Natl. Acad. Sci. USA 95 (1998) 8461-8466.
• 12. Widlak, P., Li, P., Wang, X. and Garrard, W. T. Cleavage preferences of the apoptotic endonuclease DFF40 (Caspase-activated DNase or Nuclease) on naked DNA and chromatin substrates. J. Biol. Chem. 275 (2000) 8226- 8232.
• 13. Sakahira, H., Enari, M., Ohsawa, Y., Uchiyama, Y. and Nagata, S. Apoptotic nuclear morphological change without DNA fragmentation. Curr. Biol. 9 (1999) 543-546.
• 14. Walker, P. R., Leblanc, J., Carson, C., Ribecco, M. and Sikorska, M. Neither caspase-3 nor DNA fragmentation factor is required for high molecular weight DNA degradation in apoptosis. Ann. N. Y. Acad. Sci. 887 (1999) 48-59.
• 15. Gross, D. S. and Garrard, W. T. Nuclease hypersensitive sites in chromatin. Ann. Rev. Biochem. 57 (1988) 159-197.
• 16. Gromova, I. I., Thomsen, B. and Razin, S. V. Different topoisomerase II antitumor drugs direct similar specific long-range fragmentation of an amplified c-MYC gene locus in living cells and in high-salt-extracted nuclei. Proc. Natl. Acad. Sci. USA 92 (1995) 102-106.
• 17. Toh, S. Y., Wang, X. and Li, P. Identification of the nuclear factor HMG2 as an activator for DFF nuclease activity. Biochem. Biophys. Res. Commun. 250 (1998) 598-601.
• 18. Garrard W. T. Chromosomal loop organization in eukaiyotic genomes. In: Nucleic Acids and Molecular Biology, vol. 4. (Eckstein F. and Lilley D. M. J. eds.), Springer-Verlag Berlin Heidelberg, 1990, 163-175.