Shoot regeneration from GUS-transformed tomato [Lycopersicon esculentum] hairy root

• Autorzy: Moghaieb R E A , Saneoka H. , Fujita K.
• Języki publikacji: EN

Abstrakty

EN

To study the influence of genetic background on the transformation and regeneration of cultivated tomato plants, hairy root lines of tomato (Lycopersicon esculentum) were obtained by inoculating the hypocotyl explants of three tomato cultivars with the Agrobacterium rhizogenes strain DCAR-2, which harbors the pBI-121 binary vector. The Ri-T-DNA transformation into the plant DNA was confirmed by both of mikimopine and GUS assay analyses. The regeneration efficiency from hairy root explants was assessed. The data indicated that white embryonic calli were formed within two weeks in the presence of 2 mgl-1 2, 4-D plus 0.25 mgl-1 kinetin. Adventitious shoots emerged from the embryonic callus in the presence of 1 mgl-1 GA3 along with 0.5 mgl-1 NAA. The regeneration frequency was higher in the cultivar UC-97, followed by Momotaro and then Edkawi. Molecular confirmation of the integration of the GUS gene into the hairy root-derived plants genomes was done via PCR using GUS-specific primers and also using Southern blotting analysis. Our data shows that regeneration is possible from hairy roots of the cultivated tomato and this system could be used to produce transgenic tomato plants expressing the genes present in Agrobacterium rhizogenes binary vectors.

Słowa kluczowe

EN

Lycopersicon esculentum; transformation; tomato plant; shoot regeneration; hairy root; tomato

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2004
• Tom: 09
• Numer: 3
• Opis fizyczny: p.439-449,fig.,ref.

Twórcy

autor

Moghaieb R E A

• Hiroshima University, Higashi-Hiroshima, 739-8528, Japan

autor

Saneoka H.

autor

Fujita K.

Bibliografia

• 1. Bajaj, Y.P.S. Biotechnology in Agriculture and Forestry-Somatic Embryogenesis and Synthetic Seed II. Springer-Verlag, New York, 1995, 70-80.
• 2. Chitton, M.D., Tepfer, D.A. and Petit, A. Agrobacterium rhizogenes inserts T-DNA into the genome of host plant root cells. Nature 295 (1982) 432-434.
• 3. Kato, Y., Uozumi, N., Kimura, T., Honda, H. and Kobayashi, T. Enhancement of peroxidase production and excertion from horseradish hairy roots by light, NaCl, and peroxidase-adsorption in situ. Plant Tissue Culture Lett. 8 (1991) 158-165.
• 4. Uozumi, N., Kohketsu, K., Kondo, O., Honda, H. and Kobayashi, T. Fed-bach culture of hairy root using fructose as a carbon source. J. Ferment. Bioeng. 72 (1991) 457-460.
• 5. Flores, H.E., Hoy, M.W. and Pickard, J.J. Secondary metabolites from root cultures. Trends Biotechnol. 5 (1987) 64-69.
• 6. Tepfer, D. Genetic transformation of special species of higher plants by Agrobacterium rhizogenes: phenotypic consequences and sexual transmission of transformed genotype and phenotype. Cell 37 (1984) 959967.
• 7. Ooms, G, Karp, A., Burrell, M.M., Twell, D. and Roberts, J. Genetic modification of potato development using Ri T-DNA. Theor. Appl. Genet. 70 (1985) 440-446.
• 8. Uozumi, N., Ohatake, Y., Nakashimada Y., Morikawa, Y., Tanaka, N. and Kobayashi, T. Efficient regeneration from GUS-transformed Ajuga hairy root. J. Ferment. Bioeng. 5 (1996) 374-378.
• 9. Lambert, C. and Tepfer, D. Use of Agrobacterium rhizogenes to create chimeric apple trees through genetic grafting. Biotechnol. 9 (1991) 80-83.
• 10. Hamill , J.D., Perscott, A. and Martin, C. Assessment of the efficiency of cotransformation of the T-DNA of disarmed binary vectors derived from Agrobacterium tumefaciens and the T-DNA of A. rhizogenes. Plant Mol. Biol. 9 (1987) 573-584.
• 11. Koornneef, M., Bade, J., Hanhart, C., Horsman, K., Schel, J., Soppe, W., Vekerk, R. and Zabel, P. Characterization and mapping of a gene controlling shoot regeneration in tomato. Plant J. 31 (1993) 31-141.
• 12. Meredith, C.P. Shoot development in established callus cultures of cultivated tomato (Lycopersicon esculentum Mill). Z. Pflanzenphysiol. Bd. 95 (1979) 405-411.
• 13. Hamza, S. and Chupeau, Y. Re-evaluation of conditions for plant regeneration and Agrobacterium-mediated transformation from tomato (Lycopersicon esculentum). J. Exp. Bot. 44 (1993) 1837-1845.
• 14. Moghaieb, R.E.A., Saneoka, H. and Fujita, K. Plant regeneration from hypocotyl and cotyledon explant of tomato (Lycopersicon esculentum Mill.). Soil Sci. Plant Nutr. 45 (1999) 639-646.
• 15. Peres, L.E.P., Morgante, P.G., Vecchi, C., Kraus, J.E. and Sluys, M.A. Shoot regeneration capacity from roots and transgenic hairy roots of tomato cultivars and wild related species. Plant Cell Tissue Organ Cult. 65. (2001) 37-44.
• 16. Morgan, A.J., Cox, P.N., Turner, D.A., Peel, E., Davey, M.R., Gratland, K.M.A. and Mulligan, B.J. Transformation of tomato using an Ri plasmid vector. Plant Sci. 49 (1987) 37-49.
• 17. Smith, R.M., Marshall, J.A., Davey, M.R., Lowe, K.C. and Power, J.B. Comparison of volatiles and waxes in leaves of genetically engineered tomatoes. Phytochem. 43 (1996) 753-758.
• 18. Isogai, A., Fukuchi, N., Hayashi, M., Kamada, H., Harad, H. and Suzuki, A. Mikimopine an opine in hairy roots of tobacco induced by Agrobacterium rhizogenes. Phytochemistry 29 (1990) 3131-3134.
• 19. Jefferson, R.A., Kavanagh, T.A. and Bevan, M.W. GUS fusion. ß-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6 (1987) 3901-3907.
• 20. Murashige, T. and Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15 (1962) 473-379.
• 21. Tanaka, N. Detection of opines by electrophoresis. Plant Tissue Culture Lett. 7 (1990) 45-47.
• 22. Maxwell, S.E. and Delaney, H.D. Designing Experiments and Analyzing Data. Wadsworth, Belmont, CA, 1989, 241-260.
• 23. Rogers, S.O. and Bendich, A.J. Extraction of DNA from milligram amounts of fresh herbarium and mummified plant tissues. Plant Mol. Biol. 5 (1985) 69-76.
• 24. Sambrook, J., Fritsch, E.F. and Maniatis, T. Molecular Coloning: A laboratory Manual, Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY, 1989.
• 25. Davioud, E.A., Petit, A., Tate, M.A., Ryder, M.H. and Tempe, J. Cucumopine - a new T-DNA encoded opine in hairy root and crown gall. Phytochem. 27 (1988) 2429-2433.
• 26. Tanaka, N. and Matsumoto, T. Regeneration from Ajuga hairy roots with high productivity of 20-hydroxyecdysone. Plant Cell Rep. 13 (1993) 87-90.