DNA fingerprinting of chicken lines divergently selected for high or low incidence of skeletal malformations

• Autorzy: Sacharczuk M , Parada R. , Jaszczak K.
• Języki publikacji: EN

Abstrakty

EN

Genetic analysis was conducted in chicken belonging to lines divergently selected for high or low frequency of skeletal defects (line H and L, respectively). The analysis was based on the DNA fingerprinting technique, using enzyme HinfI and Jeffrey’s 33.6 probe. Principal aims were: 1) to determine the effects of selection on the genetic variation within and between lines, and 2) to search for the presence of line-specific minisatellite alleles (as bands in the DNA fingerprinting pattern) which could be used as markers for the predisposition to skeletal defects. DNA fingerprinting pattern (DFP) displayed differences in position of bands, some of them appearing characteristic only for one out of two chicken lines studied.

PL

Przeprowadzono analizę genetyczną kur z dwóch linii, selekcjonowanych rozbieżnie na wysoką (H) i niską (L) częstość występowania wad szkieletowych. W analizie wykorzystano technikę DNA fingerprinting z użyciem enzymu HinfI i wielopozycyjnej sondy molekularnej Jeffreys’a 33.6. Głównym celem analizy było 1) określenie wpływu selekcji na zmienność genetyczną wewnątrz selekcjonowanych linii i między tymi liniami oraz 2) poszukiwanie specyficznych alleli minisatelitarnych (w postaci prążków we wzorze DNA fingerprinting) dla danej linii, które mogą być markerami predyspozycji do wad szkieletowych. W obrazie DNA fingerprinting stwierdzono prążki specyficzne tylko dla jednej bądź drugiej selekcjonowanej linii kur.

Słowa kluczowe

EN

selection; minisatellite; DNA fingerprinting; animal breeding; chicken line; genetic analysis; chicken; divergent selection; skeletal defect

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2005
• Tom: 23
• Numer: 2
• Opis fizyczny: p.119-127,fig.,ref.

Twórcy

autor

Sacharczuk M

• Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Parada R.

autor

Jaszczak K.

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