The role of actin and microtubule networks in plasmid DNA intracellular trafficking

• Autorzy: Ondrej V. , Likasova E. , Falk M. , Kozubek S.
• Języki publikacji: EN

Abstrakty

EN

This work is focused on the function of the microtubule and actin networks in plasmid DNA transport during liposomal transfection. We observed strong binding of plasmid DNA-lipid complexes (lipoplexes) to both networks and directional long-range motion of these lipoplexes along the microtubules. Disruption of either of these networks led to the cessation of plasmid transport to the nucleus, a decreased mobility of plasmids, and accumulation of plasmid DNA in large aggregates at the cell periphery. Our findings show an indispensable but different role of both types of cytoskeleton, actin and microtubular, in the processes of gene delivery.

Słowa kluczowe

EN

cytoskeleton; liposomal transfection; gene delivery; plasmid transport; microtubule; actin filament; actin network; plasmid DNA; nucleus; plasmid DNA-lipid complex

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2007
• Tom: 54
• Numer: 3
• Opis fizyczny: p.657-663,fig.,ref.

Twórcy

autor

Ondrej V.

• Academy of Sciences of the Czech Republic, Kralovopolska 135, Brno 612 65, Czech Republic

autor

Likasova E.

autor

Falk M.

autor

Kozubek S.

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