IgA and IgG antibodies in paired serum and saliva samples in human strongyloidiasis

• Autorzy: Costa I N , Sopelete M C , Goncalves-Pires M R F , Costa-Cruz J M
• Języki publikacji: EN

Abstrakty

EN

Strongyloidiasis frequently causes chronic asymptomatic disease in the gastrointestinal tract. Saliva samples have been recommended for antibody detection due to the ease of acquisition, non-invasive collection, and low risk of infection to the laboratory technicians. The present study was elaborated to examine the potential effectiveness of saliva samples for the detection of strongyloidiasis. A total of 118 paired human serum and saliva samples were analyzed to detect IgA and IgG anti- Strongyloides by the IFAT and ELISA using Strongyloides ratti antigen. By IFAT 54.2% of serum samples and 72.9% of salivary samples were specific IgA positive, while IgG positivity was 70.9% in serum and 33.3% in saliva samples. In ELISA, the positivity for serum IgA was 66.7% and 45.8% for salivary IgA, whereas specific IgG positivity was 77.1% in serum and 29.2% in saliva. It was concluded that IFAT, to detect specific IgA in saliva, and ELISA, to detect specific serum IgG, may be the more useful tests. Saliva can thus be used as an alternative fluid, in the application of IFAT, for strongyloidiasis identification.

Słowa kluczowe

EN

gastrointestinal tract; strongyloidiasis; infection; man; serum; diagnosis; Strongyloides stercoralis; asymptomatic disease; nematode; saliva; antibody

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2003
• Tom: 48
• Numer: 4
• Opis fizyczny: p.306-311,fig.,ref.

Twórcy

autor

Costa I N

• Laboratorio de Parasitologia, Instituto de Ciencias Biomedicas, Universidade Federal de Uberlandia, Av.Para 1720, Uberlandia, MG, Brasil

autor

Sopelete M C

• Laboratorio de Parasitologia, Instituto de Ciencias Biomedicas, Universidade Federal de Uberlandia, Av.Para 1720, Uberlandia, MG, Brasil

autor

Goncalves-Pires M R F

• Laboratorio de Parasitologia, Instituto de Ciencias Biomedicas, Universidade Federal de Uberlandia, Av.Para 1720, Uberlandia, MG, Brasil

autor

Costa-Cruz J M

• Laboratorio de Parasitologia, Instituto de Ciencias Biomedicas, Universidade Federal de Uberlandia, Av.Para 1720, Uberlandia, MG, Brasil

Bibliografia

• Atkins N.S., Conway D.J., Lindo J.F., Bailey J.W., Bundy D.A.P. 1999. L3 antigen-specific antibody isotype responses in human strongyloidiasis: correlations with larval output. Parasite Immunology, 21, 517-526.
• Atkins N.S., Lindo J.F., Lee M.G., Conway D.J., Bailey J.W., Robinson R.D., Bundy D.A.P. 1997. Humoral responses in human strongyloidiasis: correlations with infection chronicity. Transactions of the Royal Society of Tropical Medicine and Hygiene, 91, 609-613.
• Baermann G. 1917. Eine einfache Methode zur Auffindung von Ankylostomum (Nematoden) Larven in Erdproben. Mede-deel mit H. Geneesk. Batavia, Laboratories Weltevreden, Feestbundei, 41-47.
• Carranza F.A., Newman M.G. 1997. Mecanismos de defesa da gengiva. In: Periodontia Clínica (Eds. F.A. Carranza and M.G. Newman). Guanabara Koogan, Rio de Janeiro, 108-115.
• Conway D.J., Atkins N.S., Lillywhite J.E., Bailey J.W., Robinson R.D., Lindo J.F., Bundy D.A.P, Bianco A.E. 1993a. Immunodiagnosis of Strongyloides stercoralis infection: a method for increasing the specificity of the indirect ELISA. Transactions of the Royal Society of Tropical Medicine and Hygiene, 87, 173-176.
• Conway D.J., Bailey J.W., Lindo J.F., Robinson R.D., Bundy D.A.R, Bianco A.E. 1993b. Serum IgG reactivity with 41-, 31-, and 28-kDa larval proteins of Strongyloides stercoralis in individuals with strongyloidiasis. Journal of Infectious Diseases, 168, 784-787.
• Costa-Cruz J.M., Bullamah C.B., Gonçalves-Pires M.R.F., Campos D.M.B., Vieira M.A. 1997. Cryo-microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis. Revista do Instituto de Medicina Tropical de São Paulo, 39, 313-317.
• Coudert J., Ambroise-Thomas P., Thai K.T., Ponthier M.A. 1968. Diagnostic sérologique de l’anguillulose humaine par immuno-fluorescence (resultats préliminaires). Bulletin de la Société de Pathologie Exotique et de ses Filiales, 61,74—80.
• Dafalla A. A. 1972. The indirect fluorescence antibody test for the serodiagnosis of strongyloidiasis. Journal of Tropical Medicine and Hygiene, 75, 109-111.
• Gam A.A., Neva F.A., Krotoski W.A. 1987. Comparative sensitivity and specificity of ELISA and IHA for serodiagnosis of strongyloidiasis with larval antigens. American Journal of Tropical Medicine and Hygiene, 37, 157-161.
• Genta R.M., Gatti S., Linke M.J., Cevini C., Scaglia M. 1988. Endemic strongyloidiasis in northern Italy: clinical and immunological aspects. Quarterly Journal of Medicine, New Series 68, 257, 679-690.
• Grimoud A., Pontel F., Armaud C., Duffaut D., Lodter J.P. 1996. Dialogue avec le practicien. Dosages de protéines dans as salive: méthodologie. Option Biology, 156, 1-8.
• Grove D.I. 1996. Human strongyloidiasis. Advances in Parasitology, 38, 251-309.
• Grove D.I., Blair A.J. 1981. Diagnosis of human strongyloidiasis by immunofluorescence, using Strongyloides ratti and S. stercoralis larvae. American Journal of Tropical Medicine and Hygiene, 30, 344—349.
• Liu L.X., Weller P.F. 1993. Strongyloidiasis and other intestinal nematode infections. Infectious Disease Clinics of North America, 7, 655-682.
• Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 29, 265-275.
• Lutz A.V. 1919. Schistosoma mansoni e a schistossomose, segundo observações feitas no Brasil. Memórias do Instituto Oswaldo Cruz, 11,121-125.
• Machado E.R., Ueta M.T., Gonçalves-Pires M.R.F., Oliveira J.B.A., Faccioli L.H., Costa-Cruz J.M. 2001. Diagnosis of human strongyloidiasis using particulate antigen of two strains of Strongyloides venezuelensis in indirect immunofluorescence antibody test. Experimental Parasitology, 99, 52-55.
• McGhee J.R., Mestecky J. 1990. Em defesa das superficies de mucosa: desenvolvimento de novas vacinas para respostas protetoras da IgA em portas de entrada dos patógenos microbianos. Clinica de Doenças Infecciosas da America do Norte, 4, 303-328.
• Paula F.M., Castro E., Gonçalves-Pires M.R.F., Marçal M.G., Campos D.M.B., Costa-Cruz J.M. 2000. Parasitological and immunological diagnoses of strongyloidiasis in immunocompromised and non-immunocompromised children at Uberlândia, state of Minas Gerais, Brazil. Revista do Instituto de Medicina Tropical de São Paulo, 42, 51-55.
• Rossi C.L., Takahashi E.E., Partei C.D., Teodoro L.G., da Silva L.J. 1993. Total serum IgE and parasite specific IgG and IgA antibodies in human strongyloidiasis. Revista Instituto de Medicina Tropical de São Paulo, 35, 361-365.
• Salvi S., Holgate S.T. 1999. Could the airway epithelium play an important role in mucosal immunoglobulin A production? Clinical and Experimental Allergy, 29, 1597-1605.
• Sanchez-Martin J.R., Cardas J., Ahedo L., Fano E., Eshebarria A., Azpiroz A. 2001. Social behavior, cortisol, and sIgA levels in preschool children. Journal of Psychosomatic Research, 50, 221-227.
• Siddiqui A.A., Berk S.L 2001. Diagnosis of Strongyloides stercoralis infection. Clinical Infectious Diseases, 33, 1040-1047.
• Silva L.P., Barcelos I.S.C., Passos-Lima A.B., Espindola F.S., Campos D.M.B., Costa-Cruz J.M. 2003. Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis. Memórias do Instituto Oswaldo Cruz, 98, 687-691.
• Vacca-Smith A.M., Bowen W.H. 2000. In situ studies of pellicle formation on hydroxyapatite discs. Archives of Oral Biology, 45, 277-291.