PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2005 | 54 | 4 |

Tytuł artykułu

Selection of potentially probiotic Lactobacillus strains towards their inhibitory activity against poultry enteropathogenic bacteria

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Lactobacilli were isolated from chicken gastrointestinal tract and examined for their potentially probiotic properties towards their inhibitory activity against poultry enteropathogenic bacteria. Biochemical tests, ITS-PCR and cell wall protein analysis were used to characterize the Lactobacillus isolates. The identification of isolated Lactobacillus strains based on phenotypic properties was not always satisfactory. ITS-PCR together with protein profile were found to be helpful in strain identification. Lactobacilli were tested for the inhibitory activity against selected strains of poultry enteropathogenic bacteria (Salmonella Enteritidis, Escherichia coli and Clostridium perfringens). Examined supernatants from Lactobacillus broth cultures demonstrated major antimicrobial activity against C. perfringens. Lower antimicrobial activity were observed against E. coli and Salmonella Enteritidis. The strongest inhibition effect were obtained using supernatant of Lactobacillus acidophilus strain 3D. Results received from this study confirmed that identification of Lactobacillus spp. is often tedious. Some isolates, which are in vitro antagonistic against enteropathogenic bacteria may be considered as potential candidates for poultry probiotics, especially in controlling necrotic enteritis caused by C. perfringens.

Wydawca

-

Rocznik

Tom

54

Numer

4

Opis fizyczny

p.287-294,fig.,ref.

Twórcy

  • Warsaw Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland
autor

Bibliografia

  • Andrighetto C, P. De Dea, A. Lombardi, E. Neviani, L. Rossetti and G. Giraffa. 1998. Molecular identification and cluster analysis of homofermentative thermophilic lactobacilli isolated from dairy products. Res. Microbiol. 149: 631-643.
  • Callewaert R., H. Holo, B. Devreese, J. Van Beeumen, I. Nes and L. De Vuyst. 1999. Characterization and production of amylovorin L471, a bacteriocin purified from Lactobacillus amylovorus DCE 471 by a novel three-step method. Microbiology 145: 2559-2568.
  • Council of the European Communities. 1998. Regulation EEC no. 2821/98. Off. J. Eur. Commun., L351/4.
  • Cuozzo S.A., F. Sesma, J.M. Palacios, A.P. de Ruiz Hoigado and R.R. Raya. 2000. Identification and nucleotide sequence of genes involved in the synthesis of lactocin 705, a two-peptide bacteriocin from Lactobacillus casei CRL 705. FEMS Microbiol. Lett. 185: 157-161.
  • Daud Khaled A.K., B.A. Neilan, A. Henriksson and P.L. Conway. 1997. Identification and phylogenetic analysis of Lactobacillus using mulitiplex RAPD-PCR. FEMS Microbiol. Lett. 153: 191-197.
  • Drake M.A., C.L. Small, K.D. Spence and B.G. Swanson. 1996. Differentiation of Lactobacillus helveticus strains using molecular typing methods. Food. Res. Int. 29: 451-455.
  • Du Toit M., L.M.T. Dicks and W.H. Holzapfel. 2003. Identification of heterofermentative lactobacilli isolated from pig feaces by numerical analysis of total soluble cell protein patterns and RAPD-PCR. Lett. Appl. Microbiol. 37: 12-16.
  • Fuller R. 1992. Probiotics: The scientific basis. London, New York, Chapman & Hall.
  • Gatti M., E. Fornasari and E. Neviani. 1997. Cell-wall protein profiles of diary thermophilic lactobacilli. Lett. Appl. Microbiol. 25: 345-348.
  • Gevers D., G. Huys and J. Swings. 2001. Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol. Lett. 205: 31-36.
  • Gomes A.M.P. and F.X. Malcata. 1999. Bifidobacterium spp. and Lactobacillus acidophilus: biological, biochemical, technological and therapeutical properties relevant for use as probiotics. Trends Food Sci. Technol. 10: 139-157.
  • Guarneri T., L. Rossetti and G. Giraffa. 2001. Rapid identification of Lactobacillus brevis using the polymerase chain reaction. Lett. Appl. Microbiol. 33: 377-381.
  • Henriksson A., L. André and P.L. Conway. 1995. Distribution of lactobacilli in the porcine gastrointestinal tract. FEMS Microbiol. Ecol. 16: 55-60.
  • Jack R.W., J.R. Tagg and R. Bibek. 1995. Bacteriocins of Gram-Positive Bacteria. Microbiol. Rev. 59; 171-200.
  • Johnson J.L., C.F. Phelps, C.S. Cummins, J. London and F. Gasser. 1980. Taxonomy of the Lactobacillus acidophilus group. Int. J. Syst. Bacteriol. 30: 53-68.
  • Kullen M.J., R.B. Sanozky-Dawes, D.C. Crowell and T.R. Klaenhammer. 2000. Use of the DNA sequence of variable regions of the 16S rRNA gene for rapid and accurate identification of bacteria in the Lactobacillus acidophilus complex. J. Appl. Microbiol. 89: 511-516.
  • La Ragione R.M., A. Narbad, M.J. Gasson and M.J. Woodward. 2004. In vivo characterization of Lactobacillus johnsonii FI9785 for use as a defined competitive exclusion agent against bacterial pathogens in poultry. Lett. Appl. Microbiol. 38: 197-205.
  • Malicki K. and M. Binek. 2004. Outline of the clinical veterinary bacteriology (In Polish). SGGW , Warszawa.
  • Mukai T., K. Arihara, A. Ikeda, K. Nomura, F. Suzuki and H. Ohori. 2003. Lactobacillus kitasatonis sp. nov., from chicken intestine. Int. J. Syst. Evol. Microbiol. 53: 2055-2059.
  • Nigatu A., S. Ahrne and G. Molin. 2000. Temperature-dependent variation in API 50 CH fermentation profiles of Lactobacillus species. Curr. Microbiol. 41: 21-6.
  • Reinheimer J.A., L. Morelli, V. Bottazzi and V. Suarez. 1995. Phenotypic Variability among Cells of Lactobacillus helveticus ATCC 15807. Int. Diary J. 5: 97-103.
  • Roy D., P.Ward, D. Vincent and F. Mondou. 2000. Molecular identification of Potentially Probiotic Lactobacilli. Curr. Microbiol. 40: 40-46.
  • Sablon E., B. Contreras and E. Vandamme. 2000. Antimicrobial peptides of lactic acid bacteria: mode of action, genetics and biosynthesis. Adv. Biochem. Eng. Biotechnol. 68: 21-60.
  • Schillinger U. and F.K. Lucke. 1989. Antibacterial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol. 55: 1901-1906.
  • Spano G., L. Beneduce, D. Tarantino, G. Zapparoli and S. Massa. 2002, Characterization of Lactobacillus plantarum from wine must by PCR species-specific and RAPD-PCR. Lett. Appl. Microbiol. 35: 370-374.
  • Strompfová V, A. Lauková and D. Mudroňová. 2003, Effect of bacteriocin-like substance produced by Enterococcus faecium EF55 on the composition of avian gastrointestinal microflora. Acta Vet. Brno 72: 559-564.
  • Tsai C.C., H.Y. Hsih, H.H. Chiu, YY. Lai, J.H. Liu, B. Yu and H.Y. Tsen. 2005, Antagonistic activity against Salmonella infection in vitro and in vivo for two Lactobacillus strains from swine and poultry. Int. J. Food Microbiol. 2: 185-194.
  • Tynkkynen S., R. Satokari, M. Saarela, T. Mattila-Sandholm and M. Saxelin. 1999. Comparison of ribotyping, randomly amplified polymorphic DNA analysis, and pulse-field gel electrophoresis in typing of Lactobacillus rhamnosus and L. casei Strains. Appl. Environ. Microbiol. 65: 3908-3914.
  • Ventura M. and R. Zink. 2002. Specific identification and molecular typing analysis of Lactobacillus johnsonii by using PCR-based methods and pulsed-field gel electrophoresis. FEMS Microbiol. Lett. 217: 141-154.
  • Zapparoli G., S. Torriani and F. Dellaglio. 1998. Differentiation of Lactobacillus sanfranciscensis strains by randomly amplified polymorphic DNA and pulsed-field gel electrophoresis. FEMS Microbiol Lett. 166: 325-332.
  • Zhu M.W., W. Lin and D.Q. Wu. 2000. Isolation and characterization of a new bacteriocin from Lactobacillus gasseri KT7, J. Appl. Mcrobiol. 88: 877-886.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-4e681d02-915e-4353-9c3f-fbb34564ff4a
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.