NMR-based localization of ions involved in salting out of hen egg white lysozyme

• Autorzy: Poznanski J.
• Języki publikacji: EN

Abstrakty

EN

NaCl-induced aggregation of hen egg white lysozyme (HEWL) was monitored by NMR spectroscopy. Small, but significant, changes induced by salt addition in TOCSY spectra were attributed to the effect of local reorganization of protein backbone upon ion binding. Salt-induced variations in HN and Hα chemical shifts were mapped on the HEWL 3D structure which allowed the construction of a scheme of the spatial localization of potential ion binding sites. It was found that in a 0.5 M NaCl solution six chloride anions and at least one sodium cation are bound to preferred sites on the HEWL surface.

Słowa kluczowe

EN

hen; egg white; protein crystallization; hen egg; lysozyme aggregation

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2006
• Tom: 53
• Numer: 2
• Opis fizyczny: p.421-424,fig.,ref.

Twórcy

autor

Poznanski J.

• Polish Academy of Sciences, Warsaw, Poland

Bibliografia

• Alexander DJI (1995) Lattice Models. In Science and Technology of Crystal Growth (van der Eerden JP, Bruinsma OSL, eds) pp 81–95, Kluwer Academic Publishers, The Netherlands.
• Dauter Z, Dauter M (1999) Anomalous signal of solvent bromides used for phasing of lysozyme. J Mol Biol 289: 93–101.
• Dauter Z, Dauter M, Rajashankar KR (2000) Novel approach to phasing proteins: derivatization by short cryo-soaking with halides. Acta Crystallogr D 56: 232–237.
• Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6: 277–293.
• Evans G, Bricogne G (2002) Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu K alpha X-ray equipment. Acta Crystallogr D 58: 976–991.
• Georgalis Y, Saenger W (1999) Light scattering studies on supersaturated protein solutions. Sci Prog 82: 271–294.
• Georgalis Y, Umbach P, Zielenkiewicz A, Utzig E, Zielenkiewicz W, Zielenkiewicz P, Saenger W (1997) Microcalorimetric and small-angle light scattering studies on nucleating lysozyme solutions. J Am Chem Soc 119: 11959–11965.
• Goddard TD, Kneller DG (2003) SPARKY 3, University of California, San Francisco.
• Koradi R, Billeter M, Wuthrich K (1996) MOLMOL: A program for display and analysis of macromolecular structures J Mol Graphics 14: 51–57.
• Lim K, Nadarajah A, Forsythe EL, Pusey ML (1998) Locations of bromide ions in tetragonal lysozyme crystals Acta Crystallogr D 54: 899–904.
• Narayanan J, Liu XY (2003) Protein interactions in undersaturated and supersaturated solutions: a study using light and X-ray scattering. Biophys J 84: 523–532.
• Niimura N, Minezaki Y, Ataka M, Katsura T (1994) Small angle neutron scattering from lysozyme in unsaturated solutions, to characterize the pre-crystallization process. J Crystal Growth 137: 671–675.
• Oki H, Matsuura Y, Komatsu H, Komatsu H, Chernov AA (1999) Refined structure of orthorhombic lysozyme crystallized at high temperature: correlation between morphology and intermolecular contacts Acta Crystallogr D 55: 114–121.
• Poznański J, Georgalis Y, Wehr L, Saenger W, Zielenkiewicz P (2003) Comparison of two different lysozyme types under native and crystallization conditions using two-dimensional NMR and dynamic light scattering. Biophys Chem 104: 605–616.
• Poznański J, Wszelaka-Rylik M, Zielenkiewicz W (2004) Concentration dependencies of NaCl salting of lysozyme by calorimetric methods Thermochim Acta 409: 25–32.
• Poznański J, Szymański J, Basińska T, Słomkowski S, Zielenkiewicz W (2005a) Aggregation of aqueous lysozyme solutions followed by dynamic light scattering and 1H NMR spectroscopy. J Mol Liq 121: 21–26.
• Poznański J, Wszelaka-Rylik M, Zielenkiewicz W (2005b) HEW lysozyme salting by high-concentration NaCl solutions followed by titration calorimetry. Biophys Chem 113: 137–144.
• Price WS, Tsuchiya F, Arata Y (2001) Time dependence of aggregation in crystallizing lysozyme solutions probed using NMR self-diffusion measurements Biophys J 80: 1585–1590.
• Retailleau P, Ducruix A, Ries-Kautt M (2002) Importance of the nature of anions in lysozyme crystallisation correlated with protein net charge variation. Acta Crystallogr D 58: 1576–1581.
• Ries-Kautt MM, Ducruix AF (1989) Relative effectiveness of various ions on the solubility and crystal growth of lysozyme. J Biol Chem 264: 745–748.
• Sauter C, Otalora F, Gavira JA, Vidal O, Giege R, Garcia- Ruiz JM (2001) Structure of tetragonal hen egg-white lysozyme at 0.94 angstrom from crystals grown by the counter-diffusion method Acta Crystallogr D 57: 1119–1126.
• Schall CA, Arnold E, Wiencek JM (1996) Enthalpy of crystallization of hen egg-white lysozyme J Crystal Growth 165: 293–298.
• Sibille L, Pusey ML (1994) Investigation of nucleating lysozyme solutions. Acta Crystallogr D 50: 396–397.
• Steinrauf LK (1998) Structures of monoclinic lysozyme iodide at 1.6 angstrom and of triclinic lysozyme nitrate at 1.1 angstrom. Acta Crystallogr D 54: 767–779.
• Tanaka S, Yamamoto M, Kawashima K, Ito K, Hayakawa R, Ataka M (1996) Kinetic study on the early stage of the crystallization process of two forms of lysozyme crystals by photon correlation spectroscopy. J Crystal Growth 168: 44–49.
• Vaney MC, Broutin I, Retailleau P, Douangamath A, Lafont S, Hamiaux C, Prange T, Ducruix A, Ries-Kautt M (2001) Structural effects of monovalent anions on polymorphic lysozyme crystals. Acta Crystallogr D 57: 929–940.
• Walsh MA, Schneider TR, Sieker LC, Dauter Z, Lamzin VS, Wilson KS (1998) Refinement of triclinic hen eggwhite lysozyme at atomic resolution. Acta Crystallogr D 54: 522–546.
• Wang YJ, Bjorndahl TC, Wishart DS (2000) Complete H- 1 and non-carbonylic C-13 assignments of native hen egg-white lysozyme. J Biomol NMR 17: 83–84.
• Xiao RF, Alexander JID, Rosenberger F (1988) Morphological evolution of growing crystals: A Monte Carlo simulation. Phys Rev A 38: 2447–2456.