Cardioprotective effect of 5-lipoxygenase gene [ALOX5] silencing in ischemia-reperfusion

• Autorzy: Lisovyy O. O. , Dosenko V.E. , Nagibin V.S. , Tumanovska L.V. , Korol M.O. , Surova O.V. , Moibenko O.O.
• Języki publikacji: EN

Abstrakty

EN

 It is well known that 5-lipoxygenase derivates of arachidonic acid play an important pathogenic role during myocardial infarction. Therefore, the gene encoding arachidonate 5-lipoxygenase (ALOX5) appears to be an attractive target for RNA interference (RNAi) application. In experiments on cultivated cardiomyocytes with anoxia-reoxygenation (AR) and in vivo using rat model of heart ischemia-reperfusion (IR) we determined influence of ALOX5 silencing on myocardial cell death. ALOX5 silencing was quantified using real-time PCR, semi-quantitative PCR, and evaluation of LTC4 concentration in cardiac tissue. A 4.7-fold decrease of ALOX5 expression (P < 0.05) was observed in isolated cardiomyocytes together with a reduced number of necrotic cardiomyocytes (P < 0.05), increased number live (P < 0.05) and unchanged number of apoptotic cells during AR of cardiomyocytes. Downregulation of ALOX5 expression in myocardial tissue by 19% (P < 0.05) resulted in a 3.8-fold reduction of infarct size in an open chest rat model of heart IR (P < 0.05). Thus, RNAi targeting of ALOX5 protects heart cells against IR injury both in culture and in vivo.

Słowa kluczowe

EN

arachidonate 5-lipoxygenase; myocardial infarction; cardioprotective effect; cardiomyocyte; 5-lipoxygenase gene; arachidonic acid; ischaemia-reperfusion; RNA interference

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2009
• Tom: 56
• Numer: 4
• Opis fizyczny: p.687-694,fig.,ref.

Twórcy

autor

Lisovyy O. O.

• Bogomoletz Institute of Physiology, Kiev, Ukraine

autor

Dosenko V.E.

autor

Nagibin V.S.

autor

Tumanovska L.V.

autor

Korol M.O.

autor

Surova O.V.

autor

Moibenko O.O.

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