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1993 | 40 | 4 |
Tytuł artykułu

The amino acid>s that constitute sequence gamma 268-282 of fibrinogen are not involved in fibrin monomer polymerization

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Języki publikacji
Congenitally abnormal fibrinogens with impaired fibrin monomer polymerization have been described to contain single amino-acid substitutions localized in certain positions of the γ275-330peptide region. To evaluate the role of the amino-acid sequence in the vicinity of Arg 275 in fibrin monomer polymerization, the peptide fragment corresponding to γ268-282was synthesized and used to obtain peptide-specific antibodies. These antibodies, when purified immunochemically on the immobilized peptide, bound to the intact fibrinogen and fibrin monomers with the same binding affinity. However, they did not recognize the Y268-282epitopes on the denatured and reduced fibrinogen molecules. The lack of influence of antipeptide antibodies on fibrin monomer polymerization indicates that the γ268-282peptide is not directly involved in the structure of the polymerization site in the D domain of fibrinogen. It is suggested that substitution of Arg275either by His or Cys in abnormal fibrinogens results probably in conformational changes which disturb a proper orientation of the polymerization site and reduce its expression.
Opis fizyczny
  • Medical University in Lodz, 90-131 Lodz, Lindleya 3, Poland
  • 1. Kudryk, B.J., Collen, ., Woods, K.R. & , B. (1974) for localization of polymerization sites in fibrinogen. }. Biol. Chem. ,3322 - 3326.
  • 2. Kudryk, B., Blomback, B. & Blomback, M. (1976) Fibrinogen Detroit an abnormal fibrinogen with non functional NH2terminal polymerization domain. Thromb. Res.9,25 -36.
  • 3. Kloczewiak, M., Timmons, S., Lukas, T.J. & Hawiger, J. (1984) Platelet receptor recognition site on human fibrinogen. Synthesis and structure - function relationship of peptides corresponding to the carboxy-terminal segment of the gamma chain. Biochemistry23,1767 -1774.
  • 4. Chen, R. & Doolittle, R.F. (1971) Gamma- -crosslinking sites in human and bovine fibrin. Biochemistry10,4486 - 4491.
  • 5. Terukina, S., Matsuda, M., Hirata, H., Takeda,Y., Miyata, T., Tkae, T. & Shimonishi, Y. (1988) Substitution of y-Arg-275 by Cys in an abnormal fibrinogen, "Fibrinogen Osaka II". J. Biol. Chem. ,13579 -13587.
  • 6. Varadi, A. & Scheraga, H.A. (1986) Localization of segments essential for polymerization and for calcium binding in the y-chain of human fibrinogen. Biochemistry25,519 - 528.
  • 7. Cierniewski, C.S., Kloczewiak, M. & Budzynski, A.Z. (1986) Expression of primary polyme­rization sites in the D-domain of human fibrinogen depends on intact conformation. J. Biol. Chem.261,9116 - 9121.
  • 8. Cierniewski, C.S., Ugarova, T.P. & Budzynski, A.Z. (1991) Primary polymerization site in the y-chain of human fibrin. Thromb. Haemost.65, 675.
  • 9. Southan, C., Thompson, E., Panico, M., Etienne, T., Morris, H.R. & Lane, D.A. (1985) Characte­rization of peptides cleaved by plasmin from the C-terminal polymerization domain of human fibrinogen. J. Biol. Chem.260,13095 -13101.
  • 10. Soria, J., Soria, C., Samama, M., Tabori, S., Kehl, M., Henschen, A., Nieuwenhuizen, W., Rimon, A. & Tatarski, I. (1987) Fibrinogen Haifa: fibrinogen variant with absence of protective effect of calcium on plasmin degradation of y-chains. Thromb. Haemost.57,310 - 313.
  • 11. Reber, P., Furlan, M., Henschen, A., Kaudewitz, H., Barbui, T., Hilgard, P., Nenci, G.G., Berrettini, B. & Beck, E.A. (1986) Three abnormal fibrinogen variants with the same amino acid substitution (y275 His-Arg): Fibrinogen Bergamo II, Essen and Perugia. Thromb. Haemost.56,406 - 411.
  • 12. Matsuda, M., Yamazumi, K., Terukina, S., Onohara, S. & Ohta, Y. (1988) An abnormal fibrinogen with a yArg to His substitution found in a Japanese family, Fibrinogen Saga; in Fibrinogen 3: Biochemistry, Biological Functions, Gene Regulation an Expression (Mosesson, M.W., Amrani, D.L., Siebenlist, K.R. & DiOrio, J.P., eds.) pp. 267 - 295, Amsterdam, The Nether­lands, Elsevier Science.
  • 13. Yoshida, N., Ota, K., Moroi, M. & Matsuda, M. (1988) An apparently higher molecular weight y chain variant in a new congenital abnormal fibrinogen Tochigi characterized by the replacement of y arginine-275 by cysteine. Blood ,480-487.
  • 14. Schmelzer, C.H., Ebert, R.F. & Bell, W.R. (1988) Fibrinogen Baltimore IV: Congenital dys- fibrinogenemia with a y 275(Arg -> Lys) substitution. Thromb. Res. 56,- 317.
  • 15. Doolittle, R.F., Schubert, D. & Schwartz, S.A. (1967) Amino acid sequence studies on artiodactyl fibrinopeptides. I. Dromadary camel, mule deer, and cape buffalo. Arch. Biochem. Biophys.118,456 - 467.
  • 16. Merrifield, R.D. (1963) Solid phase peptide synthesis. I. The synthesis of tetrapeptide. J. Am. Chem. Soc.85,2149 - 2154.
  • 17. Horiki, K., Kenichi, J. & Innony, K. (1978) Synthesis of the Merrifield resin esters of N-protected amino acids with the aid of hydrogen bonding. Chem. Lett.1,165 -168.
  • 18. Kaiser, EvColessott, R.L., Bossinger, C.D. & Cook, P.I. (1970) Color test for detection of free terminal amino group in solid phase synthesis of peptides. Anal. Biochem.34,595 - 598.
  • 19. Tam, J.P. (1987) Synthesis of biologically active transforming growth factor. Int. ]. Pept. Protein Res.29,421 -431.
  • 20. Avrameas, S. & Ternynck, T. (1969) The cross-linking of proteins with glutaraldehyde and its use for the preparation of immuno- adsorbents. Immunochemistry 6,- 66.
  • 21. Cierniewski, C.S., Janiak, A. & Wyroba, E. (1986) Modification of fibrin network ultrastructure by Fab fragments specific for different domains of fibrinogen. Acta Biochim. Polon.33,195 - 202.
  • 22. Nakane, P.K. & Kawaoi, A. (1974) Peroxidase - labeled antibody. A new method of conjugation. J. Histochem. Cytochem. 22,-1092.
  • 23. Budzynski, A.Z., Olexa, S.A. & Brizuela, B.S. (1979) The interference of plasmic degradation products of human crosslinked fibrin with clot formation. Biochim. Biophys. Acta584,284 - 287.
  • 24. Itzhaki, R.F. & Gill, D.M. (1960) A micro-biuret method for estimating proteins. Anal. Biochem. ,401-410.
  • 25. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London)227,680 - 685.
  • 26. Towbin, H., Staehelin, T. & Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Set. U.S.A.76,4350 - 4354.
  • 27. Dang, C.V., Bell, W.R. & Shuman, M. (1989) The normal and morbid biology of fibrinogen. Am. ]. Med.87,567 - 577.
  • 28. Bantia, S., Mane, S.M., Bell, W.R. & Dang, C.V. (1990) Baltimore I: Polymerization defect associated with a y 292 Gly -> Val (GGC -> GTC) mutation. Blood76,2229 - 2238.
  • 29. Bantia, S., Bell, W.R. & Dang, C.V. (1990) Polymerization defect of fibrinogen Baltimore III due to a y Asn 308 -> lie mutation. Blood75, 1659 -1663.
  • 30. Yoshida, N., Terukina, S., Okuma, M., Moroi, M., Aoki,N. & Matsuda, M. (1988) Characterization of an apparently lower molecular weight y- -chain variant in fibrinogen Kyoto I. The replacement of y-asparagine 308 by lysine which causes accelerated cleavage of fragment Di by plasmin and the generation of a new plasmin cleavage site. /. Biol. Chem.263,13848 - 13856.
  • 31. Yamazumi, K., Shimura, K., Terukina, S., Takahashi, N. & Matsuda, M. (1989) A y methionine - 310 to threonine substitution and consequent N-glycosylation at y asparagine 308 identified in congenital dysfibrinogenemia associated with post traumatic bleeding, fibrinogen Asahi. /. Clin. Invest.86,1590 -1597.
  • 32. Reber, P., Furlan, M., Rupp, C., Kehl, M., Henschen, A. & Beck, E.A. (1986) Chara­cterization of fibrinogen Milano I: Amino acid exchange y 330 Asp -> Val impairs fibrin polymerization. Blood67,751 - 760.
  • 33. Bolyard, M.G. & Lord, S.T. (1991) Mutagenesis of human fibrinogen y chain 259-411 synthe­sized in E. coli.Further characterization of the role of the disulfide bond Cys326 - Cys339 in calcium binding. Biochem. Biophys. Res. Commun.174,853 - 860.
  • 34. Cierniewski, C.S. & Poniatowski, J. (1990) The effect of anti-P43-47 Fab fragments on expression of the polymerization sites in the E domain of fibrinogen. Acta Biochim. Polon.37,31 - 37.
  • 35. Zamarron, C., Ginsberg, M.H. & Plow, E.F.(1991) A receptor-induced binding site in fibrinogen elicited by its interaction with platelet membrane glycoprotein Ilb-IIIa. J. Biol. Chem.66,16193 -16199.
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