hnRNP-R regulates the PMA-induced c-fos expression in retinal cells

• Autorzy: Huang J , Li S.-J. , Chen X.-H. , Han Y. , Xu P.
• Języki publikacji: EN

Abstrakty

EN

This study focused on the function of hnRNP-R in the regulation of c-fos expression. We demonstrated that hnRNP-R accelerated the rise and decline phases of c-fos mRNAs and Fos proteins, allowing PMA to induce an augmented pulse response of c-fos expression. Then, we examined the role of the c-fos-derived AU-rich element (ARE) in hnRNP-R-regulated mRNA degradation. Studies with the ARE-GFP reporter gene showed that hnRNP-R significantly reduced the expression of GFP with an inserted ARE. Moreover, immunoprecipitation-RT-PCR analysis demonstrated that in R28 cells and rat retinal tissues, the c-fos mRNA was co-immunoprecipitated with hnRNP-R. These findings indicate that hnRNP-R regulates the c-fos expression in retinal cells, and that the ARE of c-fos mRNAs contributes to this regulation.

Słowa kluczowe

EN

immediate early gene; retina; retinal cell; spinal muscular atrophy; c-fos protein; protein expression; mRNA turnover

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2008
• Tom: 13
• Numer: 2
• Opis fizyczny: p.303-311,fig.,ref.

Twórcy

autor

Huang J

• Fudan University, 138 Yixueyuan Road, Shanghai, 200032, P. R. China

autor

Li S.-J.

autor

Chen X.-H.

autor

Han Y.

autor

Xu P.

Bibliografia

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