Przydatnosc diagnostyczna markerow uszkodzenia miesni szkieletowych u psow zaprzegowych w treningu

• Autorzy: Procajlo A

Warianty tytułu

EN

Diagnostic efficacy of indicators of muscle damage in sled dogs during training

• Języki publikacji: PL

Abstrakty

EN

Uncontrolled and over-intensive training can lead to a decrease in exercise efficiency and health state disorders in dogs. Examinations of sled dogs revealed that prolonged effort induced specific biochemical changes and released indicatory enzymes into peripheral circulation. The purpose of the study was to reveal the efficacy of selected markers of aspartate aminotransferase (AST), creatine kinase (CK), lactate dehydrogenate (LDH) and their isoenzymes, C-reactive protein (CRP), glucose and lactic acid in detecting sub clinical states of skeletal muscles lesions. Examinations were carried out on 17 sled dogs (Siberian Husky, Alaskan Malamut) at the start, during and end of the training season, before and after exercise. Dogs were in good condition before study and did not revealed clinical symptoms of disease. During progressively extended training loads a decrease in the motor activity of some dogs was noted. An increase in the activity of AST, CK and LDH5 in the examined dogs confirmed these changes and testified to skeletal muscle injury. The lack of adaptation of organism efficiency to excessive trainings loads also caused an increase in the concentration of glucose and lactic acid in the plasma of the sled dogs. C-reactive protein and inflammatory state markers were also designated to estimate of health state of the dogs. An increase of CRP concentration, noted in the examined dogs, could testified to inflammatory states of muscles or may have be connected with exercise stress. Clinical symptoms confirmed these changes. A lack of physical adaptation to the intensity of training leads to muscle injuries. Measurements of muscle injury markers during excessive load training facilitate the recognition of hyper-training states and muscles injuries in sled dogs.

Słowa kluczowe

PL

aminotransferaza asparaginianowa; miesnie szkieletowe; psy; dehydrogenaza mleczanowa; enzymy; psy pociagowe; izoenzymy dehydrogenazy mleczanowej; markery; bialko CRP; wartosc diagnostyczna; kinaza kreatynowa; uszkodzenia

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 03
• Opis fizyczny: s.306-310,tab.,bibliogr.

Twórcy

autor

Procajlo A

• Uniwersytet Warminsko-Mazurski, ul.Oczapowskiego 14, 10-957 Olsztyn

Bibliografia

• 1.Aktas M., Auguste D., Lefebvre H. P., Toutain P. L., Braun J. P.: Creatine kinase in the dog: a review. Vet. Res. Commun. 1993, 17, 353-369.
• 2.Bigoszewski M., Rychlik A., Depta A.: Białka ostrej fazy u zwierząt. Medycyna Wet. 2001, 57, 151-154.
• 3.Burr J. R., Reinhart G. A., Swenson R. A., Swaim S. F., Vaughn D. M., Bradley D. M.: Comparison of biological changes before and after racing, and between dogs competing in long distance sled dogs races. Recent Advances in Canine and Feline Nutritional Research, (wyd.) Carey D. P., Norton S. A., Bolser S. M. Orange Frazer Press, Wilmington, Ohio USA 1996, 207-218.
• 4.Burr J. R., Reinhart G. A., Swenson R. A., Swaim S. F., Vaughn D. M., Bradley D. M.: Serum biochemical values in sled dogs before and after competing in long-distance races. J. Am. Vet. Med. Assoc. 1997, 211, 175-179.
• 5.Chanoit G. P., Concordet D., Lefebvre H. P., Orcel K., Braun J. P.: Exercise does not induce major changes in plasma muscle enzymes, creatinine, glucose and total proteins concentrations in untrained beagle dogs. J. Vet. Med. 2002, 49, 222-224.
• 6.Coker R. H., Koyama Y., Denny J. C., Camacho R. C., Lacy D. B., Wasserman D. H.: Prevention of overt hypoglycemia during exercise. Diabetes 2002, 51, 1310-1318.
• 7.Hamm R.: Lactate dehydrogenase and its importance in muscle research. Fleischwirtschaft 1990, 70, 1336-1339.
• 8.Harris P. A.: Comparative aspects of exertional myopathy. Adv. Vet. Sci. 1993, 4, 115-138.
• 9.Hinchcliff K. W., Olson J., Crusberg C., Kenyon J., Long R., Royle W., Weber W., Burr J. R.: Serum biochemical changes in dogs competing in a long-distance sled race. J. Am. Vet. Med. Assoc. 1993, 202, 401-405.
• 10.Hinchcliff K. W., Shaw L. C., Vukich N. S., Schmidt K. E.: Effect of distance traveled and speed of racing on body weight and serum enzyme activity of sled dogs competing in a long-distance race. J. Am. Vet. Med. Assoc. 1998, 213, 639-644.
• 11.Hinchcliff K. W.: Energy and water expenditure. Proceedings of the performance dog nutrition symposium. Colorado 1995, 18, 4-9.
• 12.Ilkiw J. E., Davis P. E., Church D. B.: Hematologic, biochemical, blood-gas, and acid-base values in Greyhounds before and after exercise. Am. J. Vet. Res. 1989, 50, 583-586.
• 13.Kędzierski W., Podolak M.: Wpływ treningu koni rasy arabskiej na poziom parametrów biochemicznych związanych z gospodarką węglowodanowo-lipidową. Medycyna Wet. 2002, 58, 788-791.
• 14.Kittleson M. D., Johnson L. E., Pion P. D.: Submaximal exercise testing using lactate threshold and venosus oxygen tension as end points in normal dogs and in dogs with heart failure. J. Vet. Intern. Med. 1996, 10, 21-27.
• 15.Kostro K., Gliński Z., Krakowski L., Wojcicka-Lorenowicz K.: Przydatność w diagnostyce oznaczania białek ostrej fazy u psów i kotów. Mag. Wet. 2001, 57, 42-44.
• 16.Milne E. M., Doxey D. L.: Lactate dehydrogenase and its isoenzymes in the tissues and sera of clinically normal dogs. Res. Vet. Sci. 1987, 43, 222-224.
• 17.Querengaesser A., Iben C., Leibetseder J.: Blood changes during training and racing in sled dogs. J. Nutr. 1994, 124, 2760S-2764S.
• 18.Reynolds A. J., Carey D. P., Reinhart G. A., Swenson R. A., Kallfelz F. A.: Effect of post exercise carbohydrate supplementation on muscle glycogen repletion in trained sled dogs. Am. J. Vet. Res. 1997, 58, 1252-1256.
• 19.Reynolds A. J., Fuhrer L., Dunlap H. L., Finke M., Kallfelz F. A.: Effect of diet and training on muscle glycogen storage and utilization in sled dogs. J. Appl. Physiol. 1995, 79, 1601-1607.
• 20.Scott-Moncrieff J. C., Hawkins E. C., Cook J. R.: Canine muscle disorders. Compend Cont. Ed. Pract. Vet. 1990, 12, 31-39.
• 21.Stopyra A.: Wskaźniki gospodarki tlenowej i aktywność wybranych enzymów surowicy koni w warunkach ekstremalnego wysiłku. Medycyna Wet. 2002, 58, 543-548.
• 22.Stuewe S. R., Gwirtz P. A., Agarwal N., Mallet R. T.: Exercise training enhances glycolytic and oxidative enzymes in canine ventricular myocardium. J. Mol. Cell. Cardiol. 2000, 32, 903-913.
• 23.Väihkönen L. K., Heinonen O. J., Hyyppä S., Nieminen M., Pösö R.: Lactate - transport activity in RBCs of trained and untrained individuals from four racing species. Am. J. Physiol. 2001, 281, R19-R24.
• 24.Valentine B. A., Blue J. T., Cooper B. J.: The effect of exercise on canine dystrophic muscle. Ann. Neurol. 1989, 26, 588.
• 25.Weibel E. W., Taylor C. R., Weber J., Vock J., Roberts R., Hoppeler H.: Design of the oxygen and substrate pathways VII. Different structural limits for oxygen supply to muscle mitochondria. J. Exp. Biol. 1996, 199, 1699.
• 26.Winnicka A.: Wartości referencyjne podstawowych badań laboratoryjnych w weterynarii. Wyd. SGGW, Warszawa 2002.
• 27.Zinker B. A., Wilson R. D., Wasserman D. H.: Interaction of decreased arterial PO₂ and exercise on carbohydrate metabolism in the dog. Am. J. Physiol. 1995, 269, 409-417.