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1997 | 38 | 2 |

Tytuł artykułu

Estimation of the share of components of different ploidy in anisoploid sugar-beet [Beta vulgaris L.] populations by flow cytometry

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Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Flow cytometry is commonly used in sugar-beet breeding and seed production for ploidy estimation. By means of this method individual plants as well as plant populations can be analysed. An interpretation of histograms obtained from anisoploid populations, however, causes some problems to the breeder. Bulks of diploid, triploid and tetraploid sugar-beet plants in different proportion and ploidy combinations (2x + 3x, 2x + 4x, and 3x + 4x) were tested in order to determine the relation between the actual share of the components of different ploidy levels in tested populations and that calculated on the basis of flow cytometry. In this way agraph for estimation of the share of different ploidy components was obtained. Repeatability of the results proves the usefulness of flow cytometric analysis of anisoploid plant populations in breeding and seed production of polyploid crops.

Wydawca

-

Rocznik

Tom

38

Numer

2

Opis fizyczny

p.151-160,fig.

Twórcy

autor
  • Department of Genetics and Plant Breeding, University of Technology and Agriculture, Kaliskiego 7, 85-796 Bydgoszcz, Poland
autor
  • Kleinwanzlebener Saatzucht AG, Einbeck, Germany

Bibliografia

  • Akinerdem F. (1991). Determination of the ploidy level of pure and mixed plant populations of sugar beet (Beta vulgaris L.) by flow cytometry. Plant Breeding 107: 333-337.
  • Beaumont V.H., Widholm J.M. (1993). Ploidy variation of pronamide-treated maize calli during long term culture. Plant Cell Rep. 12: 648-651.
  • Brown S.C., Bergounioux C.,Tallet S., Marie D. (1991a). Flow cytometry of nuclei for ploidy and cell cycle analysis. In: A Laboratory Guide for Cellular and Molecular Plant Biology. (Negrutuin I., Gharli-Chhetri G. eds.). Birkhäuscr Verlag, Basel: 326-345.
  • Brown S.C., Devaux P., Marie D., Bergounioux C., Petit P.X. (1991b). Cytométrie en flux: Application à l'analyse de la ploidie chez les végétaux. Le Tcchnoscopc dc Biofutur 47: 2-14.
  • Galbraith D.W. (1989). Analysis of higher plants by flow cytometry and cell sorting. Int. Rev. Cytol. 116: 165-228.
  • Galbraith D.W., Harkins K.R., Maddox J.M., Ayres N.M., Sharma D.P., Firoozabady E. (1983). Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049-1051.
  • Laat de A.M.M., Göhde W., Vogelzang M.J.D.C. (1987). Determination of ploidy of single plants and plant populations by flow cytometry. Plant Breeding 99: 303-307.
  • Śliwińska E. (1993). Cytometria przepływowa - nowa metoda analizy cyklu komórkowego i genomu roślin wyższych. Hodowla Roślin i Nasiennictwo 1: 10-15.
  • Śliwińska E„ Steen P. (1995). Flow cytometric estimation of ploidy in anisoploid sugar-beet populations. J. Appl. Genet. 36: 111-118.
  • Uijtewaal B.A. (1987). Ploidy variability in greenhouse cultured and in vitro propagated potato (Solanum tuberosum) monohaploids (2n=x=12) as determined by flow cytometry. Plant Cell Rep. 6: 252-255.

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Bibliografia

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