The effect of selected heavy metal ions on the growth and conidial germination of the aphid pathogenic fungus Pandora neoaphidis [Remaudiere et Hennebert] Humber

• Autorzy: Tkaczuk C
• Języki publikacji: EN

Abstrakty

EN

The effect of heavy metal ions (Cd, Cr³⁺, Cu², Ni, Pb²⁺ and Zn) on the growth and germination of conidia of the aphid pathogenic fungus Pandora neoaphidis was studied. The metal ions were added into the culture medium in three concentrations: A – concentration corresponding to the mean content of that metal in Polish soils, B – concentration 10-times higher and C – 100-times higher than the mean ones. The investigated heavy metal ions, except for Ni, added to the media at the concentrations corresponding to the mean content of these metals in Polish soils, did not affect the growth of aphid-pathogenic fungus Pandora neoaphidis. Ni, Cu, Zn and Cr, added to the media at a concentration 100-times higher than in Polish soils, prevented the growth of the pathogen. All the tested metal ions, except for Cu, added to the media at the mean soil concentration, did not affect conidial germination of the fungus. The conidia of P. neoaphidis were unable to germinate in the presence of the Cr, Cu, Pb and Zn ions in the medium at a concentration that was 100-times higher than the mean one. Cu and Zn caused a significant reduction of conidial germination even at a concentration that was 10-times higher than the mean content of these metals in Polish soils. This work suggest that strong pollution of soil by some heavy metals could be a restrictive factor of development and pathogenicty of entomophthoralean fungi in the environment.

Słowa kluczowe

EN

Pandora neoaphidis; conidial germination; Entomophthorales; mycelium growth; aphid population; pathogenic fungi; metal ion; fungi; growth; aphid; heavy metal; germination

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2005
• Tom: 14
• Numer: 6
• Opis fizyczny: p.897-902,fig.,ref.

Twórcy

autor

Tkaczuk C

• University of Podlasie, Prusa 14, 08-110 Siedlce, Poland

Bibliografia

• 1. KELLER S., SUTER H. Epizootiologishe Untersuchungen über das Entomophthora-Auftreten bei feldbaulich wichtigen Blattlausarten. Acta Oecolog. Appl. 1, 63, 1980.
• 2. DEDRYVER C. A., Biology of cereal aphids in Western France. II. Spatial-temporal distribution and the limiting action of three species of Entomophthoraceae., Entomophaga, 26, 381, 1981.
• 3. REMAUDIÉRE G., LATGÉ J. P., MICHEL M. F. Comparative ecology of Entomophthoraceae pathogenic to aphids in costal and inland France. Entomophaga. 26, 157, 1981.
• 4. WILDING N., BRADY B. L. Erynia neoaphidis, in Commonwealth Mycological Institute, Descriptions of Pathogenic Fungi and Bacteria no. 815. The Cambrian News, Aberystwyth, UK, 1984.
• 5. LATGÉ J. P., SILVIE P., PAPIEROK B., REMAUDIÉRE G., DEDRYVER C. A., RABASSE J. M. Advantages and disadvantages of Conidiobolus obscurus and of Erynia neoaphidis in the biological control of aphids. In: Cavalloro R. (ed.) Aphid Antagonists. A. A. Balkema, Rotterdam. 20, 1983.
• 6. GLARE T. R., MILNER R. J. Ecology of entomopathogenic fungi, in Handbook of Applied Mycology, Vol. 2: Humans, Animals and Insects (ARRORA D. K., AJELLO L. & MUKERJI K. G., Eds.) Marcel Dekker Inc., New York. 1991.
• 7. KELLER S. Arthropod–pathogenic Entomophthorales of Switzerland. II. Erynia, Eryniopsis, Neozygites, Zoophthora and Tarichium. Sydowia 43, 39, 1991.
• 8. SHAH P. A., AEBI M., TUOR U. Method to immobilize the aphid-pathogenic fungus Erynia neoaphidis in an alginate matrix for biocontrol. Appl. Environ. Microbiol. 64 (11), 4260, 1998.
• 9. MIĘTKIEWSKI R. T., PELL J. K., CLARK S. J. Influence of pesticide use on the natural occurrence of entomopathogenic fungi in arable soils in the UK: field and laboratory comparison. Biocontrol Sci. Technol. 7, 565, 1997.
• 10. TKACZUK. Effects of selected pesticides on the germination of conidia of the aphid entomopathogenic fungus Erynia neoaphidis Remaudiere et Hennebert. Aphids and Other Hemipterous Insects. 9, 137, 2003.
• 11. MAJCHROWICZ I., POPRAWSKI T. J. Effects of herbicides on in vitro vegetative growth and sporulation of entomopathogenic fungi. Crop Protection, 14 (1), 81, 1995.
• 12. ZIMMERMAMM G. Effects of systemic fungicides on aphid-infecting Entomophthoraceae (Zygomycetes) in vitro. Z. Pflanzen-Krankh Pflanzenschutz. 83, 261, 1976.
• 13. WILDING N. The effects of systemic fungicides on the aphid pathogen, Cephalosporium aphidicola. Plant Pathol., 21, 137, 1972.
• 14. ROSIN F. Effects of fertilizers on the survival of Beauveria bassiana. J. Invertebr. Pathol. 68, 194, 1996.
• 15. BÅÅTH E. Tolerance of copper by entomogenous fungi and the use of copper-amended media for isolation of entomogenous fungi from soil. Mycol. Res. 95 (9), 1140, 1991.
• 16. NORDGREN A., BÅÅTH E., SÖDERSTRÖM B. Soil microfungi in an areas polluted by heavy metals. Can. J. Bot. 63, 448, 1985.
• 17. JAWORSKA M., JASIEWICZ CZ., GORCZYCA A. Effect of heavy metal ions in Silesia’s soil on entomopathogenic micro-organisms. Progress in Plant Protection/Post. W Ochr. Rośl. 37 (2), 276, 1997. (in Polish).
• 18. ROPEK D., PARA A. The effect of heavy metal ions and their complexons upon the growth, sporulation and pathogenicity of the entomopathogenic fungus Verticillium lecanii. J. Invertebr. Pathol. 79, 124, 2002.
• 19. JAWORSKA M., GORCZYCA A. Metal ions influence on entomopathogenic fungi sporulation. Chem. Inż. Ekol. 11 (4-5), 341, 2004.
• 20. POPOWSKA – NOWAK E., SOSAK-ŚWIDERSKA B., BAJAN C., BIEŃKOWSKI P. Response of isolates of entomopathogenic fungus Metarhizium anisopliae to heavy metal pollution and their accumulative abilities. Chem. Inż. Ekol. 11 (1), 71, 2004.
• 21. JAWORSKA M., RADKOWSKA A., ROPEK D., TOMASIK P. Effect of metal ions on Paecilomyces fumosoroseus. IOBC/WPRS Bull. 19 (9), 221, 1996.
• 22. TKACZUK C., MIĘTKIEWSKI R., KRÓLAK E. The effect of selected metal ions on the frowth of entomopathogenic fungi Beauveria bassiana Bals. (Vuill.) and Beauveria brongniartii (Petch). Chem. Inż. Ekol. 6 (7), 645, 1999 (in Polish).
• 23. TKACZUK C. Effect of selected metal ions on the growth and germination of entomopathogenic fungus Paecilomyces fumosoroseus (Wize) Brown et Smith. Chem. Inż. Ekol. 10 (3-4), 323, 2003.
• 24. ARNEBRANT K., BÅÅTH E., SÖDERSTRÖM B. Copper tolerance of microfungi isolated from polluted and unpolluted forest soil. Mycologia 79, 890, 1987.
• 25. ROPEK D., FRĄCZEK K. Effect of soil contamination with heavy metals on the occurrence and pathogenicity of entomopathogenic nematodes and fungi in the vicinity of municipal landfill site in Tarnów. Chem. Inż. Ekol. 10 (3- 4), 309, 2003.
• 26. KABATA-PENDIAS A., PIOTROWSKA M., MOTOWICKA-TERELAK T., MALISZEWSKA-KORDYBACH B., FILIPIAK K., KRAKOWIAK A., PIERTUCH C. The bases of estimation of chemical soils pollution. Metale ciężkie, siarka i WWA. Biblioteka Monitoringu Środowiska, Warszawa, 1995 (in Polish).
• 27. BOCZEK J., SZLENDAK E. The effect of plant’s stresses on infestation of plants by pests. Post. Nauk Roln., 2, 4, 1992. (in Polish).
• 28. JAWORSKA M., GOSPODAREK J. The effect of soil contamination with heavy metals on aphids fabae (Scop.) on seed fodder beets (beta vulgaris L.). Progress in Plant Protection/Post. W Ochr. Rośl. 42 (2), 629, 2002.
• 29. HAJEK A. Ecology of terrestrial fungal entomopathogens. Advances in Microbial Ecology. 15, 193, 1997.
• 30. FENG M. G., NOWIERSKI R. M., KLEIN R. F., SCHAREN A. L., SANDS D. C. Spherical hyphal bodies of Pandora neoaphidis (Remaudiere and Hennebert) Humber (Zygomycetes: Entomophthorales) on Acyrthosiphon pisum Harris (Homoptera: Aphididae): a potential overwintering form. Pan-Pacific Entomol. 68, 100, 1992.
• 31. FENG M. G., JOHNSON J. B., HALBERT S. E. Natural contron of cereal aphids (Homoptera: Aphididae) by entomopathogenic fungi (Zygomycetes: Entomophthorales) and parasitoids (Hymenoptera: Braconidae and Encyrtidae) on irrigated spring wheat in southwestern Idaho. Environm. Entomol. 20, 1699, 1991.
• 32. MCLEOD P. J., STEINKRAUS D. C., CORRELL J. C., MORELOCK T. E. Prevalence of Erynia neoaphidis (Entomophthorales: Entomophthoraceae) infections of green peach aphid (Homoptera: Aphididae) on spinach in the Arkansas River Valley. Environm. Entomol. 27, 796, 1998.
• 33. NIELSEN C., HAJEK A., HUMBER R. A., EILENBERG J. Soil – a natural source of entomophthoralen fungi infecting aphids. IOBC/WPRS Bull. 21, 45, 1998.
• 34. TKACZUK C., MIĘTKIEWSKI R., KRÓLAK E. The effect of metal ions on the growth of selected entomopathogenic fungi. IOBC/WPRS Bull. 21 (4), 151, 1998.
• 35. TKACZUK C. Effects of selected pesticides on the growth and germination of conidia of the aphid pathogenic fungus Erynia neoaphidis Remaudiere at Hennebert. 37th Annual Meeting of Society for Invertebrate Pathology, Helsinki, 1- 6 August 2004, 63, 2004.
• 36. TOBIN J. M., WHITE C., GADD G. M. Metal accumulation by fungi: applications in environmental biotechnology. J. Industr. Microbiol. 13, 126, 1994.
• 37. GADD G. M. Interaction of fungi with toxic metals. New Phytol. 124, 24, 1993.
• 38. BABICH H., STOTZKY G. Effect of cadmium on interaction between fungi and bacteria in soil. Influence of clay minerals on pH. Appl. Environ. Microbiol. 33, 1058, 1977.
• 39. LEYVAL C., TURNAU K., HASELWANDTER K. Effect of heavy metal pollution on mycorrhizal colonization and function: physiological, ecological and applied aspects. Mycorrhiza, 7, 139, 1997.
• 40. BERTHELIN J., MUNIER-LAMY C., LEYVAL C. Effect of microorganisms on mobility of heavy metals in soils. In: Huang P. M., Berthelin J., Bollag J. M., McGill W. B., Page A. L. (eds) Metals, other inorganics, and microbial activities, (Environmental impacts of soil component interactions vol. 2) Lewis, Boca Raton, Fla, 3, 1995.
• 41. TREVORS J. T., STRATTON G. W., GADD G. M. Cadmium transport, resistance and toxicity in algae, bacteria and fungi. Can. J. Microbiol. 32, 447, 1986.
• 42. FARKAS V. The fungal cell wall. In: Fungal protoplasts. Peberdy J. F., Ferenczy L. (eds), Marcel Dekker, New York, 3, 1985.
• 43. JAWORSKA M., GORCZYCA A. Effect of metal ions on entomopathogenic fungi pathogenicity. Chem. Inż. Ekol. 11 (4-5), 327, 2004.