QTL mapping of Fusarium moniliforme ear rot resistance in maize. 1. Map construction with microsatellite and AFLP markers

• Autorzy: Zhang F , Wan X Q , Pan G T
• Języki publikacji: EN

Abstrakty

EN

To map the QTLs of Fusarium moniliforme ear rot resistance in Zea mays L., a total of 230 F₂ individuals, derived from a single cross between inbred maize lines R15 (resistant) and Ye478 (susceptible), were genotyped for genetic map construction using simple sequence repeat (SSR) markers and amplified fragment length polymorphism (AFLP) markers. We used 778 pairs of SSR primers and 63 combinations of AFLP primers to detect the polymorphisms between parents, R15 and Ye478. From the polymorphic 30 AFLP primer combinations and 159 SSR primers, we scored 260 loci in the F₂ population, among which 8 SSR and 13 AFLP loci could not be assigned to any of the linkage groups. An integrated molecular genetic linkage map was constructed by the remaining 151 SSR and 88 AFLP markers, which distributed throughout the 10 linkage groups of maize and spanned the genome of about 3463.5 cM with an average of 14.5 cM between two markers. On 4 chromosomes, we detected 5 putative segregation distortion regions (SDRs), including 2 new ones (SDR₂ and SDR₇). The other 3 SDRs were located near the regions where gametophyte genes were mapped, indicating that segregation distortion could be partially caused by gametophytic factors.

Słowa kluczowe

EN

segregation distortion; linkage map; Zea mays; segregation distortion region; simple sequence repeat; Fusarium moniliforme; genome map; maize; amplified fragment length polymorphism

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2006
• Tom: 47
• Numer: 1
• Opis fizyczny: p.9-15,fig.,ref.

Twórcy

autor

Zhang F

• Maize Research Institute, Sichuan Agricultural University, Ya'an, Sichuan, China
• Key Laboratory of Crop Genetics Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya'an, Sichuan, China

autor

Wan X Q

• Department of Forestry, Sichuan Agricultural University, Ya'an, Sichuan, China

autor

Pan G T

• Maize Research Institute, Sichuan Agricultural University, Ya'an, Sichuan, China
• Key Laboratory of Crop Genetics Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya'an, Sichuan, China

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