Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2006 | 28 | 6 |
Tytuł artykułu

Evaluation of microwave radiation method for sample preparation of pine needles and wood for carbohydrate analysis

Warianty tytułu
Języki publikacji
The aim of the current study was to test the suitability ofmicro- wave heating for stopping carbohydrate transformations in plant material. Needles and branches of Pinus sylvestris were treated in microwave oven (2.45 GHz, 800W) and compared to the samples treated in boiling ethanol (96 %). In extracts obtained from the microwaved material the ratio of sucrose to hexoses (glucose and fructose) decreased, while ethanol treatment resulted in stable extracts. The carbohydrate composition in dry samples estimated after a month of storage was persistent. The boiling of needles in ethanol in microwave oven gave the same results as the boiling on a heating plate. In the woody material, differently from the needles, the total concentration of measured carbohydrates depended significantly on the preparation method. In the case of needles, the treatment of plant material in ethanol was better suited for the determination of carbohydrate levels than the microwave treatment.
Opis fizyczny
  • Estonian University of Life Sciences, Viljandi mnt.18B, 11216 Tallinn, Estonia
  • Bergmeyer H.-U. 1988. Methods of Enzymatic Analysis. 3rd Edition. Wiley-VCH, Weinheim.
  • Corsaro A., Chiacchio U., Pistara V., Romeo G. 2004. Microwave-assisted chemistry of carbohydrates. Cur. Org. Chem. 8: 511-538.
  • Das R.H., Nahar P. 2002. Simple, efficient, and accelerated method for enzyme-catalyzed in vitro modification and synthesis of nucleic acid using microwave irradiation. United States patent no 20030232342.
  • Egger B., Einig W., Schlereth A., Wallenda T., Magel E., Loewe A., Hampp R. 1996. Carbohydrate metabolism in one- and two-year-old spruce needles, and stem carbohydrates from three months before until three months after bud break. Physiol. Plant. 96: 91-100.
  • Hoch G., Richter A., Korner C. 2003. Non-structural carbon compounds in temperate forest trees. Plant Cell Environ. 26: 1067-1081.
  • Horobin R.W. 1998. Probtems and artitacts of microwave accelerated procedures in neurohistotechnology and resolutions. Methods 15: 101-106.
  • Kerepesi I., Tóth M., Boross L. 1996. Water-soluble carbohydrates in dried plant. J. Agric. Food Chem. 44: 3235-3239.
  • Kldseiko J. 2003. Carbohydrate metabolism of conifers in alkalised growth conditions. Doctoral Thesis. Estonian Agricultural University, Tartu.
  • Kldseiko J. 2005. Application of microwave radiation for sample preparation of pine needles for carbohydrate analysis. Proc. Est. Acad. Sci. Ecol. Biol. 54: 126-135.
  • Marani E. 1998. Microwave applications in neuromorphology and neurochemistry: safety precautions and techniques. Methods 15: 87-99.
  • Martins S.I.F.S. 2003. Unravelling the Maillard reaction network by multiresponse kinetic modelling. Ph.D. Dissertation. Wageningen University, The Netherlands.
  • Narayana Murthy U.M., Sun W.Q. 2000. Protein modification by Amadori and Maillard reactions during seed storage: roles of sugar hydrolysis and lipid peroxidation. J. Exp. Bot. 51: 1221-1228.
  • Ogiwara I., Ohtsuka Y., Yoneda Y., Sakurai K., Hakoda N., Shimura I. 1999. Extraction method by water followed by microwave heating for analyzing sugars in strawberry fruits. J. Jpn. Soc. Hort. Sci. 68: 949-953.
  • Orthen B., Wehrmeyer A. 2004. Seasonal dynamics of non-structural carbohydrates in bulbs and shoots of the geophyte Galanthus nivalis. Physiol. Plant. 120: 529-536.
  • Pinkrova J., Hubackova B., Kadlec P., Prihoda J., Bubnik Z. 2003. Changes of starch during microwave treatment of rice. Czech J. Food Sci. 21: 176-184.
  • Popp M., Lied W., Meyer A.J., Richter A., Schiller P., Schwitte H. 1996. Sample preservation for determination of organic compounds: microwave versus freeze-drying. J. Exp. Bot. 47: 1469-1473.
  • Popp M., Janett H.-P., Luttge U., Medina E. 2003. Metabolite gradients and carbohydrate translocation in rosette leaves of CAM and C3 bromeliads. New Phytol. 157: 649-656.
  • Singh V., Sethi R., Tewari A., Srivastava V. 2003. Hydrolysis of plant seed gums by microwave irradiation. Carboh. Polym. 54: 523-525.
  • Steen E., Larsson K. 1986. Carbohydrates in roots and rhizomes of perennial grasses. New Phytol. 104: 339-346.
  • Turkina M.V., Sokolova S.V. 1971. Methods for the determination of mono- and oligosaccharides. In: Biochemical Methods in Plant Physiology. Nauka, Moskva: 7-34.
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.