SDS-PAGE characteristics of protein kinases tightly associated with chick embryo brain ribosomes

• Autorzy: Sanecka-Obacz M.
• Języki publikacji: EN

Abstrakty

EN

Protein kinases tightly associated with chick embryo brain ribosomes washed with Triton X-100 and KCl were characterized by their ability to phosphorylate ribosomes and two exogenous substrates, histone IIA and casein. c-AMP-dependent kinase (PKA) and casein kinases (CK1, CK2) were examined in the presence of specific modulators by SDS/ PAGE followed by renaturation in gel assay according to Kameshita & Fujisawa (Anal. Biochem. 1989, 183, 139-143). Basing on these data it can be presumed that PKA activity increases, but the levels of CK2 and CK1 decrease during chick embryo development.

Słowa kluczowe

EN

brain development; brain; phosphorylation; ribosome; protein phosphorylation; ribosomal kinase; protein; ribosomal protein; chicken; embryo; protein synthesis

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 4
• Opis fizyczny: p.911-917,fig.

Twórcy

autor

Sanecka-Obacz M.

• Medical Academy, 20-123 Lublin, Lubartowska 85, Poland

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