Isolation of low-molecular-weight glutenin subunit genes from wild emmer wheat [Triticum dicoccoides]

• Autorzy: Yue Y -W , Long H. , Liu Q. , Wei Y.-M. , Yan Z.-H. , Zheng Y.-L.
• Języki publikacji: EN

Abstrakty

EN

Three low-molecular-weight glutenin subunit (LMW-GS) genes, designated LMW-Tdl, LMW-Td2 and LMW-Td3, were isolated from wild emmer wheat (Triticum dicoccoides), which is the tetraploid progenitor of common wheat (T. aestivum). The complete nucleotide sequence lengths of LMW-Td1, LMW-Td2 and LMW-Td3 are 858, 900 and 1062 bp, respectively. LMW-Td1 and LMW-Td3 can encode proteins with 284 and 352 amino acid residues, respectively, whereas LMW-Td2 is a putative pseudogene due to the presence of 3 inframe stop codons in its C-terminal domain. The deduced protein sequences of the 3 genes share the same typical polypeptide structures with known LMW-GS genes containing 8 cysteines in the mature protein domains. LMW-Td1 was clearly distinguished from all known LMW-GS genes, and considered as a novel LMW-GS gene. Two hydrophobic motifs (i.e. PIIIL and PVIIL) were observed in the repetitive domain of LMW-Td3. Sequence comparison indicates that sequences of the 3 LMW-GS genes from this study are strongly similar to known LMW-GS genes. Our phylogenetic analysis suggests that LMW-Td1 and LMW-Td2 are homologous with genes on chromosome 1A, and LMW-Tdl is closely related to genes on chromosome 1B.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2005
• Tom: 46
• Numer: 4
• Opis fizyczny: p.349-355,fig.,ref.

Twórcy

autor

Yue Y -W

• Sichuan Agricultural University, Dujiangyan, Sichuan 611830, China

autor

Long H.

autor

Liu Q.

autor

Wei Y.-M.

autor

Yan Z.-H.

autor

Zheng Y.-L.

Bibliografia

• Bietz JA, Wall JS, 1973. Isolation and characterization of gliadin-like subunits from glutenins. Cereal Chem 50: 537-547.
• Cassidy BG, Dvorak J, Anderson OD, 1998. The wheat low-molecular-weight glutenin genes: characterization of six genes and progress in understanding gene family structure. Theor Appl Genet 96: 743-750.
• Ciaffi M, Lafiandra D, Porceddu E, Benedettelli S, 1993. Storage-protein variation in wild emmer wheat (Triticum turgidum ssp. dicoccoides) from Jordan and Turkey. I. Electrophoretic characterization of genotypes. Theor Appl Genet 86: 474-480.
• Ciaffi M, Lee Y-K, Tamas L, Gupta R, Skerritt J, Appels R, 1999. The low-molecular-weight glutenin subunit proteins of primitive wheat III. The genes from D-genome species. Theor Appl Genet 98: 135-148.
• D'Ovidio R, Masci S, 2004. The low-molecular-weight glutenin subunits of wheat gluten. J Cereal Sci 39: 321-339.
• D'Ovidio R, Tanzarella OA, Porceddu E, 1992. Nucleotide sequence of a low-molecular-weight glutenin from Triticum durum. Plant Mol Biol 18: 781-784.
• Devos KM, Alkinson MD, Chinoy CN, Liu CJ, Gale MD, 1992. RFLP-based genetic map of the homologous group 3 chromosomes of wheat and rye. Theor Appl Genet 83: 931-937.
• Gupta RB, MacRitchie F, 1994. Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1 of common wheats. II. Biochemical basis of the allelic effects on dough properties. J Cereal Sci 19: 19-29.
• Huang Z, Long H, Yan ZH, Wei YM, Zheng YL, 2005. Sequence analysis of LMW-GS genes from Aegilops tauschii containing HMW glutenin sub- units 5+12 with good quality. High Technology Letters 15: 67-72.
• Ikeda TM, Nagamine T, Fukuoka H, Yano H, 2002. Identification of new low-molecular-weight glutenin subunit genes in wheat. Theor Appl Genet 104: 680-687.
• Jackson EA, Holt LM, Payne PI, 1983. Characterization of high-molecular-weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and the chromosomal localization of their controlling genes. Theor Appl Genet 66: 29-37.
• Johal J, Gianibelli MC, Rahman S, Morell MK, Gale KR, 2004. Characterization of low-molecular-weight glutenin genes in Aegilops tauschii. Theor Appl Genet 109: 1028-1040.
• Kreis M, Shewry PR, Forde BG, Forde J, Miflin BJ, 1985. Structure and evolution of seed storage proteins and their genes, with particular reference to those of wheat, barley and rye. Oxford Surv Plant Cell Mol Biol 2: 253-317.
• Lee YK, Bekes F, Gupta R, Appels R, Morell MK, 1999. The low-molecular-weight glutenin subunit proteins of primitive wheats. I. Variation in A-genome species. Theor Appl Genet 98: 119-125.
• Long H, Wei YM, Yan ZH, Zheng YL, 2004. Molecular cloning of a novel low-molecular-weight glutenin subunit gene from wheat (Triticum aestivum L.) variety "chuannong 16". Acta Agro Sin 30: 1179-1184.
• Masci S, D'Ovidio R, Lafiandra D, Kasarda DD, 1998. Characterization of a low-molecular-weight glutenin subunit gene from bread wheat and the corresponding protein that represents a major subunit of the glutenin polymer. Plant Physiol 118: 1147-1158.
• Pagnotta MA, Nevo E, Beiles A, Porceddu E, 1995. Wheat storage proteins: glutenin diversity in wild emmer, Triticum dicoccoides, in Israel and Turkey. 2. DNA diversity detected by PCR. Theor Appl Genet 91: 409-414.
• Ruiz M, Carrillo JM, 1995. Separate effects on gluten strength of Gli-1 and Glu-3 prolamine genes on chromosomes 1A and IB in durum wheat. J Cereal Sci 21: 137-144.
• Sambrook J, Fritsch EF, Maniatis T, 1989. Molecular cloning: A laboratory manual. Cold Spring Harbour, New York.
• Shewry PR, 1993. Biological and evolutionary aspects of cereal seed storage proteins. In: Shewry PR, Stobart AK, eds. Seed storage compounds: Biosynthesis, interactions and manipulation, Oxford University Press, UK: 52-78.
• Shewry PR, Halford NG, 2002. Cereal seed storage proteins: structures, properties and role in grain utilization. J Exp Bot 53: 947-958.
• Shewry PR, Halford NG, Tatham AS, 1992. High molecular weight subunits of wheat glutenin. J Cereal Sci 15: 105-120.
• Shewry PR, Tatham AS, Barro F, Barcelo P, Lazzeri P, 1995. Biotechnology of bread-making: unraveling and manipulating the multi-protein gluten complex. Biotechnology 13: 1185-1190.
• Singh NK, Shepherd KW, 1988. The structure and genetic control of a new class of disulphide-linked proteins in wheat endosperm. Theor Appl Genet 71: 79-92.
• Wang ZQ, Long H, Zheng YL, Yan ZH, Wei YM, Lan XJ, 2005. Cloning and analysis of LMW-GS genes from Triticum aestevum ssp. tibetanum Shao. Acta Genetica Sinica 32: 86-93.