The DFF40-CAD endonuclease and its role in apoptosis

• Autorzy: Widlak P.
• Języki publikacji: EN

Abstrakty

EN

The sequential generation of large-scale DNA fragments followed by internucleosomal chromatin fragmentation is a biochemical hallmark of apoptosis. One of the nucleases primarily responsible for genomic DNA fragmentation during apoptosis is called DNA Fragmentation Factor 40 (DFF40) or Caspase-activated DNase (CAD). DFF40/CAD is a magnesium-dependent endonuclease specific for double stranded DNA that generates double strand breaks with 3'-hydroxyl ends. DFF40/CAD is activated by caspase-3 that cuts the nuclease's inhibitor DFF45/ICAD. The nuclease preferentially attacks chromatin in the internucleosomal linker DNA. However, the nuclease hypersensitive sites can be detected and DFF40/CAD is potentially involved in large-scale DNA fragmentation as well. DFF40/CAD-mediated DNA fragmentation triggers chromatin condensation that is another hallmark of apoptosis.

Słowa kluczowe

EN

cell; caspase; nuclease; caspase-activated nuclease; apoptosis; caspase-activated DNase; endonuclease; DNA fragmentation

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2000
• Tom: 47
• Numer: 4
• Opis fizyczny: p.1037-1044,fig.,ref.

Twórcy

autor

Widlak P.

• Center of Oncology, 44-100 Gliwice, Poland

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