Neurony splotu miednicznego samca swini - organizacja przestrzenna oraz immunohistochemiczna charakterystyka, ze szczegolnym uwzglednieniem neuronow zaopatrujacych nasieniowod

• Autorzy: Kaleczyc J
• Języki publikacji: PL

Abstrakty

PL

W niniejszej pracy opisano organizację przestrzenną neuronów splotu miednicznego niedojrzałego płciowo samca świni, a także przedstawiono rezultaty badań dotyczących neurochemicznego kodowania tych komórek nerwowych. Opisano również rozmieszczenie neuronów tego splotu unerwiających nasieniowód oraz neurochemiczne cechy tej grupy neuronów. U wszystkich badanych zwierząt stwierdzano obecność większego zwoju - ganglion pelvinum anterior (GPA) - zlokalizowanego w kącie zawartym pomiędzy cewkowym końcem miednicznego odcinka nasieniowodu i częścią doczaszkową gruczołu pęcherzykowego. Pozostałe neurony miedniczne splotu były zgrupowane w zwojach, których ogół tworzył dosyć zwartą strukturę o skomplikowanej budowie. Struktura ta składała się z dwóch pasm zwojów - prawego i lewego, biegnących po bokach i wzdłuż miednicznego odcinka przewodu moczopłciowego, w kącie utworzonym przez ten przewód i gruczoły - pęcherzykowy, a doogonowo - opuszkowo-cewkowy. Wspomniany GPA zapoczątkowywał te pasma od stromy doczaszkowej. Stwierdzono ponadto, iż lewe i prawe pasma zwojów połączone były kilkoma (3-5) pasmami zwojów przebiegających poprzecznie, o kształcie podków wygiętych ku górze, leżących na dogrzbietowej powierzchni miednicznego odcinka przewodu moczopłciowego, pod gruczołami płciowymi dodatkowymi. Przy użyciu techniki wstecznego znakowania neuronów fluorescencyjnym tracerem Fast Blue (FB) wykazano, iż zwoje miedniczne samca świni zawierają wiele neuronów zaopatrujących nasieniowód. Większość FB-pozytywnych (FB+) neuronów unerwiających lewy nasieniowód zlokalizowana była po stronie badanego narządu (ipsilateralnie), ale także, w mniejszej liczbie, po stronie przeciwległej (kontralateralnie) zarówno w GPA, jak i wielu innych zwojach. W badaniach immunohistochemicznych stosowano immunofluorescencyjne barwienia pojedyncze i podwójne, metodę Tramu oraz porównywanie skrawków stycznych, barwionych w kierunku różnych substancji w celu określenia ich współwystępowania w tych samych strukturach nerwowych. Badania te wykazały, iż GPA jest zwojem „mieszanym", składającym się w mniej więcej równej liczbie z neuronów zarówno adrenergicznych, zawierających enzymy toru katecholaminowego (hydroksylazę tyrozyny - TH i β-hydroksylazę dopaminy - DβH), jak i nieadrenergicznych. Jednakże zdecydowana większość zlokalizowanych w GPA neuronów zaopatrujących nasieniowód świni należała do populacji neuronów adrenergicznch. Stwierdzono ponadto, że neurony GPA samca świni zawierają, oprócz enzymów toru katecholaminowego, również wiele neuropeptydów, które mogą współwystępować w tych komórkach nerwowych. Neuropeptydami najczęściej występującymi lub współwystępującymi w neuronach adrenergicznych (także w subpopulacji neuronów unerwiających nasieniowód) były Met5-enkefalina-Arg6- Gly7-Leu8 (MEAGL) i neuropeptyd Y (NPY), w mniejszym zaś stopniu galanina (GAL). Neuropeptydami najczęściej występującymi lub współwystępujacymi w neuronach nieadrenergicznych, w tym także w neuronach FB+, były NPY - naczynioaktywny peptyd jelitowy (VIP) i somatostatyna (SOM) oraz w znacznie mniejszym stopniu peptyd syntetyzowany na genie kalcytoniny (CGRP). W badanych zwojach splotu miednicznego stwierdzono obecność dużej liczby żylakowatych włókien nerwowych zawierających CGRP i jednocześnie substancję P (SP). Włókna te zaopatrywały szczególnie intensywnie neurony adrenergiczne, a zatem i zdecydowaną większość neuronów FB+. Włókna nerwowe zawierające inne badane substancje były znacznie mniej liczne, a pojedyncze na ogól zakończenia nerwowe zaopatrywały niektóre neurony zarówno adrenergiczne, jak i nieadrenergiczne.

EN

This paper deals with the distribution and immunohistochemical characteristics of neu­rons in the pelvic plexus of the juvenile male pig. The arrangement and neurochemical coding of pelvic neurons supplying the vas deferens are also described. The investigations revealed the presence of a larger ganglion (called the anterior pelvic ganglion, GPA) in the angle between the urethral end of the vas deferens and the cranial end of the seminal vesicle. The remaining pelvic neurons were located caudally and clustered in ganglia which altogether formed a compact, but complex structure. The structure consisted of left and right stripes of ganglia distributed on and along lateral sides of the pelvic part of the urogenital duct. The stripes occupied the angles between the laterally protruding parts of the seminal vesicles and bulbourethral glands, and the urogenital duct. The stripes were interconnected with transversal, horse shoe-shaped bands (3-5) of ganglia which ran on the upper surface, of the urogenital duct under the accessory genital glands. By means of the retrograde tracing technique using the fluorescent tracer Fast Blue (FB) the pelvic ganglia were found to contain many neurons supplying the vas deferens. FB-positive (FB+) neurons occurred in both left and right GPAs and other ganglia, however, the majority of them were located ipsilaterally. The immunohistochemistry included single- and double-labelling immunofluorescence, combined sometimes with Tramu method. Additionally, con­secutive sections stained for different substances were compared to investigate the colocalization of the antigens. It has been found that the GPA is a "mixed" ganglion consisting of both adrenergic, containing catecholamine-synthesizing enzymes (tyrosine hydroxylase, TH and dopamine β-hydroxylase, DβH), and roughly the same number of non-adrenergic neurons. The vast majority of FB+ neurons belonged to the subpopulation of adrenergic neurons. Besides the catecholamine-synthesizing enzymes, pelvic neurons were found to contain some neuropeptides that often co-existed within these nerve cell bodies. Met5-enkephalin-Arg6-Gly7-Leu8 (MEAGL), neuropeptide Y (NPY), and, to a lesser extent, galanin (GAL) were the peptides most often occurring and co-localizing within adrenergic, including FB+, neurons. Non-adrenergic neurons, including FB+ nerve cells, usually contained NPY, vasoactive intestinal polypeptide (VIP) and/or somatostatin (SOM) and, less frequently, calcitonin gene-related peptide (CGRP). Numerous varicose nerve fibres containing both CGRP and substance P (SP) were detected within the ganglia studied. These fibres, however, intensely innervated only adrenergic neurons, thus, also the vast majority of FB-positive nerve cells. Nerve fibres positive to other substances investigated were definitely less numerous and they often supplied both adrenergic and non-adrenergic neurons with single nerve fibres.

Słowa kluczowe

PL

immunohistochemia; enzymy toru katecholaminowego; anatomia zwierzat; trzoda chlewna; samce; nasieniowod; splot miedniczny; neurony; neuropeptydy

EN

immunohistochemistry; catecholamine synthesizing enzyme; animal anatomy; pig; male; vas deferens; pelvic plexus; neuron; neuropeptide

• Wydawca: -
• Czasopismo: Acta Academiae Agriculturae ac Technicae Olstenensis. Veterinaria
• Rocznik: 1997
• Tom: 24,Suppl.A
• Opis fizyczny: s.1-54,rys.,fot.,tab.,tabl.,bibliogr.

Twórcy

autor

Kaleczyc J

• Akademia Rolniczo-Techniczna, Olsztyn

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