The platelet activating factor triggers preconditioning-like cardioprotective effect via mitochondrial K-ATP channels and redox-sensible signaling

• Autorzy: Penna C. , Mognetti B. , Tullio F. , Gattullo D. , Mancardi D. , Pagliaro P. , Alloatti G.
• Języki publikacji: EN

Abstrakty

EN

Endogenous platelet activating factor (PAF) is involved in heart ischemic preconditioning. PAF can also afford pharmacological preconditioning. We studied whether mitochondrial-ATP-sensitive K+ (mKATP) channels and reactive oxygen species (ROS) are involved in PAF-induced cardioprotection. In Group 1 control hearts, Langendorff-perfused rat hearts underwent 30 min ischemia and 2 hours of reperfusion. Group 2 hearts, before ischemia, were perfused for 19 min with PAF (2x10-11 M); Groups 3 and 4 hearts were co-infused with PAF and N-acetyl-L-cysteine or 5-hydroxydecanoate to scavenge ROS or to block mKATP channels, respectively. Left ventricular pressure and infarct size were determined. PAF-pretreatment reduced infarct size (33 ± 4% vs 64 ± 4.6 % of the area at risk of control hearts) and improved pressure recovery. Infarct-sparing effect of PAF was abolished by N-acetyl-L-cysteine and 5-hydroxydecanoate. Thus, the cardioprotective effect exerted by PAF-pretreatment involves activation of mKATP channels and redox signaling in pre-ischemic phase.

Słowa kluczowe

EN

myocardial infarction; platelet-activating factor; mitochondrion; reactive oxygen species; preconditioning; ischaemia-reperfusion; ischaemic preconditioning; heart

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2008
• Tom: 59
• Numer: 1
• Opis fizyczny: p.47-54,fig.,ref.

Twórcy

autor

Penna C.

• University of Turin, Turin, Italy

autor

Mognetti B.

autor

Tullio F.

autor

Gattullo D.

autor

Mancardi D.

autor

Pagliaro P.

autor

Alloatti G.