An interdisciplinary study on the mode of action of probiotics in pigs

• Autorzy: Simon O.
• Języki publikacji: EN

Abstrakty

EN

An interdisciplinary research group granted by the German Research Foundation (FOR 438) tested various hypotheses and tired to develop a model for the mode of action of probiotics in pigs. The study included the fields of animal nutrition/digestion physiology, anatomy and histology of the intestinal mucosa, transport and secretory properties of the mucosa, microbiology of the intestinal tract, immune system (classes of intraepithelial lymphocytes, humoral responses), gene expression of the mucosa and finally the in vitro and in vivo resistance against infection with Salmonella. Five trials with ten sows per treatment each and their piglets and two probiotic strains were included in this study. The studied bacterial strains were Enterococcus faecium NCIMB 10415 and Bacillus cereus var. toyoi NCIMB 40112. Concluding from our studies and the published data of others, the effects of probiotics on performance are rarely significant. However, with one exception the incidence of post-weaning diarrhoea under the effect of both probiotics was significantly reduced in the trials of the research group. Furthermore, the identification frequency of various E. coli sero-pathovars relevant in post weaning diarrhoea was reduced in these animals. On the other hand, no significant modifications were found for the morphology and histology of the intestinal mucosa and also not on transport properties of this tissue. A further important finding was that the mode of action for probiotics is not unique but species or even strain specific. Most probably the studied probiotics act directly and/ or via modifications of the intestinal microbiota on the immune system (intraepithelial lymphocyte population).

Słowa kluczowe

PL

animal feeding; pig; probiotic; digestive physiology; intestinal mucosa; mucosa; secretory property; immune system; intestinal tract; gene expression; disease resistance; Salmonella; piglet; probiotic strain; performance; Enterococcus faecium; Bacillus versus

• Wydawca: -
• Czasopismo: Journal of Animal and Feed Sciences
• Rocznik: 2010
• Tom: 19
• Numer: 2
• Opis fizyczny: p.230-243,fig.,ref.

Twórcy

autor

Simon O.

• Freie Universitat Berlin, Brummerstr.34, D-14195 Berlin, Germany

Bibliografia

• Baum B., Liebler-Tenorio E.M., Enss M.L., Pohlenz J.F., Breves G., 2002. Saccharomyces boulardii and Bacillus cereus var. toyoi influence the morphology and the mucins in the intestine of pigs. Z. Gatroenterol. 40, 277-284
• Breves G., Walter C., Burmester M., Schröder B., 2000. In vitro studies on the effects of Saccharomyces boulardii and Bacillus cereus var. toyoi on nutrient transport in pig jejunum. J. Anim. Physiol. Anim. Nutr. 84, 9-20
• Casey P.G., Gardiner G.E., Casey G., Brandshaw B., Lawlor P.G., Lynch P.R., Leonard F.C., Stanton C., Ross R.P., Fitzgerald G.F., Hill C., 2007. A fife strain probiotic combination reduces pathogen shedding and alleviates disease sings in pigs challenged with Salmonella enteritica serovar Typhimurium. Appl. Environ. Microbiol. 73, 1858-1863
• Fuller R., 1989. Probiotics in man and animals. J. Appl. Bacteriol. 66, 365-378 *Klär I., 2008. Einfluss der Verfütterung des Probiotikums E. faecium NCIMB 10415 im frühen postnatalen Stadium auf die Zusammensetzung und Stoffwechselaktivität der gastrointestinalen Mikrobiota bei Ferkeln. Dissertation, Freie Universität Berlin, ISBN: 978-3-86664-494-6
• Klein U., Schmidts H.L., 1997. Zum Einfluss des Bioregulators Parciflor auf die Morphologie der Dünndarmmukosa beim Schwein. Proc. Soc. Nutr. Physiol. 61, 41 (Abstr.)
• *Kraatz M., Taras D., 2008. Veillonella magna sp. nov., isolated from the jejunal mucosa of a healthy pig, and emended description of Veillonella ratti. Int. J. Syst. Evol. Microbiol. 58, 2755-2761
• *Kraatz M., Wallace R.J., Svensson L., 2010. Olsenella umbonata sp.nov., a microaerotolerant anaerobic lactic acid bacterium from the sheep rumen and pig jejunum, and emended description of Olsenella, Olsenella uli and Olsenella profuse. Int. J. Syst. Evol. Microbiol. (accepted) *Lodemann U., Hübener K., Jansen N., Martens H., 2006. Effects of Enterococcus faecium NCIMB 10415 as probiotic supplement on intestinal transport and barrier function of piglets. Arch. Anim. Nutr. 60, 35-48
• *Lodemann U., Lorenz B.M., Weyrauch K.D., Martens H., 2008. Effects of Bacillus cereus var. toyoi as probiotic feed supplement on intestinal transport and barrier function in piglets. Arch. Anim. Nutr. 62, 87-106
• *Macha M., Taras D., Vahjen W., Arini A., Simon O., 2004. Specific enumeration of the probiotic strain Enterococcus faecium NCIMB 10415 in the intestinal tract and in faeces of piglets and sows. Arch. Anim. Nutr. 58, 443-452
• *Nordhoff M., Taras D., Macha M., Tedin K., Busse H.-J., Wieler L.H., 2005. Description of Treponema berlinense sp. nov. and Treponema porcinum sp. nov., two novel spirochaetes isolated from porcine faeces. Int. J. Syst. Bact. 55, 1675-1680
• *Pollmann M., Nordhoff M., Pospischil A., Tedin K., Wieler L.H., 2005. Effects of a probiotic strain of Enterococcus faecium on the infection rate of Chlamydia in swine. Infect. Immunity 73, 4346-4353
• *Reiter K., Eggebrecht S., Drewes B., Riess M., Weyrauch K.D., 2006. Effects of Enterococcus faecium and Bacillus cereus var. toyoi on the morphology of the intestinal mucous membrane in piglets. Biologia (Bratisl) 61, 803-809
• Sanders M.E., 2000. Consideration for the use of probiotic bacteria to modulate human health. J. Nutr. 130, 384S-390S
• *Scharek L., Altherr B.J., Tölke C., Schmidt M.F.G., 2007b. Influence of the probiotic Bacillus cereus var. toyoi on the intestinal immunity of piglets. Vet. Immunol. Immunopathol. 120, 136-147
• *Scharek L., Guth J., Filter M., Schmidt M.F.G., 2007c. Impact of the probiotic bacteria Enterococcus faecium NCIMB 10415 (SF68) and Bacillus cereus var. toyoi NCIMB 40112 on the development of serum IgG and faecal IgA of sows and their piglets. Arch. Anim. Nutr. 61, 223-234
• *Scharek L., Guth J., Reiter K., Weyrauch K.D., Taras D., Schwerk P., Schierack P., Schmidt M.F., Wieler L.H., Tedin K., 2005. Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets. Vet. Immunol. Immunopathol. 105, 151-161
• *Schierack P., Filter M., Scharek L., Toelke C., Taras D., Tedin K., Haverson K., Lübke-Becker A., Wieler L.H., 2009. Effect of Bacillus cereus var. toyoi on immune parameters of pregnant sows. Vet. Immunol. Immunopathol. 127, 26-37
• *Schierack P., Nordhoff M., Pollmann M., Weyrauch K.D., Amasheh S., Lodemann U., Jores J., Tachu B., Kleta S., Blikslager A., Tedin K., Wieler L.H., 2006. Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine. Histochemistry Cell Biol. 125, 293-305
• *Schierack P., Steinrück H., Kleta S., Vahjen W., 2006. Virulence factor gene profiles of Escherichia coli isolates from clinically healthy pigs. Appl. Environ. Microbiol. 101, 1357-1366
• *Schierack P., Wieler L.H., Taras D., Herwig V., Tachu B., Hlinak A., Schmidt M.F.G., Scharek L., 2007. Bacillus cereus var. toyoi enhanced systemic immune response in piglets. Vet. Immunol. Immunopathol. 118, 1-11
• *Scholven J., Taras D., Sharbati-Tehrani S., Gabler C., Huber O., Meyer zum Bueschenfelde D., Blin N., Einspanier R., 2009. Intestinal expression of TFF and related genes during postnatal development in a piglet probiotic trial. Cell. Physiol. Biochem. 23, 143-156
• Simon O., Jadamus A., Vahjen W., 2001. Probiotic feed additives – effectiveness and expected modes of action. J. Anim. Feed Sci. 10, Suppl. 1, 51-67
• *Szabó I., Wieler L.H., Tedin K., Scharek-Tedin L., Taras D., Hensel A., Appel B., Nöckler K., 2009. Influence of a probiotic strain of Enterococcus faecium on Salmonella Typhimurium DT104 infection in swine as animal model. Infec. Immunity 75, 2621-2628
• *Taras D., Vahjen W., Macha M., Simon O., 2005. Response of performance characteristics and fecal consistency to long-lasting dietary supplementation with the probiotic strain Bacillus cereus var. toyoi to sows and piglets. Arch. Anim. Nutr. 59, 405-417
• *Taras D., Vahjen W., Macha M., Simon O., 2005.2006. Performance, diarrhea incidence, and occurrence of Escherichia coli virulence genes during long-term administration of a probiotic Enterococcus faecium strain to sows and piglets. J. Anim. Sci. 84, 608-617
• *Taras D., Vahjen W., Simon O., 2007. Probiotics in pigs - modulation of their intestinal distribution and of their impact on health and performance. Livest. Sci. 108, 229-231
• *Taras D., Vahjen W., Simon O., 2007. Effect the probiotic Enterococcus faecium NCIMB10415 on cell numbers of total Enterococcus spp., E. faecium and E. faecalis in the intestine of piglets. Curr. Issues Intest. Microbiol. 8, 1-7
• Wiese F., Simon O., Weyrauch K.D., 2003. Morphology of the small intestine of weaned piglets and a noval method for morphometric evaluation. Anat. Histol. Embryol. 32, 102-109
• *These publications result from the research group (FOR 438) of the German Research Foundation “Integrative Analysis of the Mode of Action of Probiotics in Pigs”