The effect of superoxide dismutase knockout on the oxidative stress parameters and survival of mouse erythrocytes

• Autorzy: Grzelak A , Kruszewski M. , Macierzynska E. , Piotrowski L. , Pulaski L. , Rychlik B. , Bartosz G.
• Języki publikacji: EN

Abstrakty

EN

The erythrocytes of 12-month old Sod1 −/− mice showed an increased level of reactive oxygen species (ROS), as estimated by the degree of dihydroethidine and dihydrorhodamine oxidation, and the increased level of Heinz bodies. No indices of severe oxidative stress were found in the red blood cells and blood plasma of Sod1 −/− mice as judged from the lack of significant changes in the levels of erythrocyte and plasma glutathione, plasma protein thiol and carbonyl groups and thiobarbituric-acid reactive substances in the blood plasma. However, a decreased erythrocyte lifespan, increased reticulocyte count and splenomegaly were noted, indicating the importance of superoxide dismutase for maintaining erythrocyte viability. The levels of erythrocyte ROS and Heinz bodies and the reticulocyte count were indistinguishable in Sod1 +/+ and Sod1 +/− mice, suggesting that a superoxide dismutase activity decrease to half of its normal value may be sufficient to secure the protective effects of the enzyme.

Słowa kluczowe

EN

superoxide dismutase; oxidative stress; stress parameter; survival; mouse; mice; erythrocyte; reactive oxygen species; acetylcholinesterase; erythrocyte level

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2009
• Tom: 14
• Numer: 1
• Opis fizyczny: p.23-34,fig.,ref.

Twórcy

autor

Grzelak A

• University of Lodz, Banacha 12/16, 90-237 Lodz, Poland

autor

Kruszewski M.

autor

Macierzynska E.

autor

Piotrowski L.

autor

Pulaski L.

autor

Rychlik B.

autor

Bartosz G.

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