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2006 | 28 | 6 |
Tytuł artykułu

Exogenous abscisic acid increases stability of polysomes in embryos of triticale caryopses during germination

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Some posttranslational processes that occur in embryos of germinating triticale caryopses treated with different concentrations of abscisic acid (ABA) were examined. ABA increased the ratio of cytoskeleton-bound polysomes in the total population of polysomes and depressed the share of free and membrane-bound polysomes. Using exogenous RNase, stability of the total polysomal population as well as each polysomal fraction was investigated. The total extractable polysomes isolated from embryonic tissues of germinating triticale caryopses treated with ABA were more stable than the polysomes isolated from the control sample caryopses. The contribution of the polysomes that were not digested by RNase was increased by higher concentrations of ABA applied during germination. At high concentrations of ABA (50, 100 pM), the quantitative contribution of polysomes in the total ribosomal fraction was almost 100 % ofthe amount ofpolysomes before digestion and the modifications observed consisted mainly of the shift of the socalled heavy polysomes towards light polysomes, containing a few ribosomes. Within each polysomal population, cytoskeleton-bound polysomes (CBP and CMBP) were the most stable, which may imply that the bonds between polysomes and these protein filaments, created in all eukaryotic cells increased their stability. It is assumed that mRNAs are stabilised or destabilised by interaction of proteins with their various sequences. A plant hormone may depress or elevate the quantities of these proteins, thus regulating the stability of different mRNAs. The results confirm the multi-faceted mechanism of ABA-induced response, where one of the constituents is the effect ofABA on the stability ofmRNAs molecules. The coordinated regulation ofmRNAs synthesis and their stability provide plants with improved adaptability.
Wydawca
-
Rocznik
Tom
28
Numer
6
Opis fizyczny
p.627-634,fig.,ref.
Twórcy
autor
  • University of Warmia and Mazury in Olsztyn, M.Oczapowskiego St.1A, 10-957 Olsztyn-Kortowo, Poland
autor
autor
autor
Bibliografia
  • Abe S., Azama K., Sugimoto H., Davies E. 2003. Protein accumulation in the maize endosperm: role of polyribosomes and the cytoskeleton. Plant Physiol. Biotchem. 41: 125-131.
  • Abe S., Davies E. 1995. Isotation of a putative rybosome-cytoskeleton binding protein from peas and corn endosperm. Plant Physiol. Biochem. 33: 463-472.
  • Abe S., Ito Y., Davies E. 1992. Co-sedimentation of actin, tubulin and membranes in cytoskeleton fractions from peas and mouse 3T3 cells. J. Exp. Bot., 43: 941-949.
  • Abe S., Davies E. 1991. Isolation of F-actin from pea stems: Evidence from fluorescence microscopy. Protoplasma 163: 51-61.
  • Biddulph T.B., Mares D.J., Plummer J.A., Setter T.L. 2005. Drought and high temperature increases preharvest sprouttng tolerance in a genotype without grain dormancy. Euphytica. 143: 277-283.
  • Black M. 1991. Involvement of ABA in the physiology of developing and mature seeds. In: Abscisic acid physiology and biochemistry, ed by W.J. Davies, H.G. Jones. BIOS Sci. Pub. Limit. Oxford, UK: 99-124.
  • Brown T.A. 1999. Genomes. BIOS Sci. Publ. Limit. Ox ford, UK.
  • Davies E., Abe S. 1995. Methods for isolation and analysis of polyribosomes. Meth. Cell. Biol. 50: 209-236.
  • Davies E., Abe S., Larkins B.A., Clore A.M., Quatrano R.S., Weidner S. 1998. The role of the cytoskeleton in plant protein synthesis. In: A Look Beyond Transcription in Plants, ed. by J. Bailey-Serres, D.R Gallic, American Society of Plant Physiologists, Rockville, Maryland, USA: 115-124.
  • Decker C.J., Parker R. 1993. A turnover pathway for both stable and unstable mRNAs in yeast: evidence for a requirement for deadenylation. Genes Dev. 7: 1632-1643.
  • Garcia-Hernandez M., Davies E., Baskin T.J., Stas- wick P.E. 1996. Association of p40 with ribosomes is enhanced when polyribosomes form during periods of active tissue growth. Plant Physiol. 111: 559-568.
  • Garcia-Hernandez M., Staswick P.E., Davies E. 1994. Arabidopsis p40 homolog: A novel acidic protein associated with 40S ribosomal sub-units. J. Biol. Chem. 269: 20744-20749.
  • Green P.J. 1993. Control of mRNA stability in higher plants. Plant Physiol. 112: 1065-1070.
  • Gualerzi C., Cammarano P. 1969. Comparative electrophoretic studies on the protein of chloroplast and cytoplasmic ribosomes of spinach leaves. Biochim. Biophys. Acta, 190; 170-186.
  • Herrick D., Parker R., Jacobson A. 1990. Identification and comparison of stable and unstable mRNAs in Saccharomyces cerevisiae. Mol. Cell. Biol. 10: 2269-2284.
  • Klyachko N., Aksenova L., Dunaeva M., Kulikova A. 2000. Interaction of plant polytomes with the actin cytoskeleton. Cell Biol. Int. 24: 351-358.
  • Mitchell P., Tollervey D. 2000. mRNA stability in eukaryotes. Curr. Opinion Gen. Develop. 10: 193-198.
  • Ross J. 1995. mRNA stability in mammatian cells. Microbiol Rev. 59: 423-450.
  • Ross J. 1996. Control of messenger RNA stability in higher eukaryots. Trends Genet. 12: 171-175.
  • Scheer I., Axelos M., Pont-Lexica R.F. 1996. Characterization of a ribosomal p40 homologue gene showing the same pattern of expression as the elongation factor - 1α in Arabidopsis thaliana L. Plant Physiol. Biochem. 34: 501-508.
  • Surdej P., Riedl A., Jacobs-Lorena M. 1994. Regulation of mRNA stabihty in development. Annu. Rev. Genet. 28: 263-282.
  • Thomas T.L., Vivekanada J., Bogue M.A. 1991. ABA regutation of gene expression in embryos and mature plants. In: Abscisic acid physiology and biochemistry, ed by W.J. Davies, H.G. Jones, BIOS Sci. Pub. Limit. Oxford, UK: 125-135.
  • Weidner S., Frączek E., Romanowska M., Amarowicz R., Abe S., Davies E. 2003. The influence of abscisic amid on different polysomal populations in embryonal tissue during pea seeds germination. Acta. Physiol. Plant., 25: 5-12.
  • Weidner S., Łukaszewicz D., Amarowicz R. 2000. Significant role for polysomes associated with the cytoskeleton in the control of protein synthesis during germination of triticale caryopses in the presence of abscisic acids. Acta Physiol. Plant., 22: 185-193.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-47d6e3af-5f15-4d94-aa47-16de3891c947
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