Chlorogenic acid in in vitro cultures of Eleutherococcus senticosus

• Autorzy: Floryanowicz-Czekalska K , Podsedek A. , Wysokinska H.
• Języki publikacji: EN

Abstrakty

EN

The establishment of callus and cell suspension cultures of Eleutherococcus senticosus was reported. The four callus lines were obtained from stolon and bud explants from a fieldgrown plant. They were cultured on Murashige and Skoog (MS) or Schenk and Hildebrandt (SH) agar media, supplemented with 30 g/l sucrose, 2,4-dichlorophenoxyacetic acid (2,4-D) (1 mg/l), α-naphtaleneacetic acid (NAA; 1 mg/l) and 6-benzylaminopurine (BAP; 0.5 mg/ l). The cell suspension culture was also maintained in SH liquid medium with the same growth regulators and sucrose (50 g/l). HP LC analysis showed that the cultures produced chlorogenic acid. The highest accumulation of the compound was detected in the cell culture (29.7 mg/g d.w.); it was 15 times the average level reported for starting callus, several times as much as roots, and far more than leaves of intact plants.

PL

Otrzymano kultury kalusowe i zawiesinowe Eleutherococcus senticosus. Cztery linie kalusowe uzyskano z rozłogów i pąków rośliny rosnącej w gruncie. Kultury hodowano na agarowym podłożu Murashige-Skooga (MS) lub Schenka i H ildebrandta (SH) wzbogaconym w kwas 2,4-dichlorofenoksyoctowy (2,4-D; 1 mg/l), kwas α-naftylo-1-octowy (NAA; 1 mg/l) i 6-benzyloaminopurynę (BAP; 0,5 mg/l). Kultura zawiesinowa była prowadzona w płynnym podłożu SH z tymi samymi regulatorami wzrostu i ze zwiększoną do 50 g/l zawartością sacharozy. Za pomocą analizy HP LC wykazano, że wszystkie kultury produkowały kwas chlorogenowy. Największą zawartość tego kwasu wykryto w kulturze zawiesinowej (29,7 mg/g suchej masy). Była ona 15 razy wyższa niż w tkance kalusowej, kilkakrotnie wyższa niż w korzeniach i znacznie wyższa niż w liściach roślin gruntowych.

Słowa kluczowe

EN

cell suspension culture; woody shrub; Eleutherococcus senticosus; chlorogenic acid; Siberia; Araliaceae; leaf; Asia; root; callus tissue; plant

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2006
• Tom: 52
• Numer: 1-2
• Opis fizyczny: p.67-74,fig.,ref.

Twórcy

autor

Floryanowicz-Czekalska K

• Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland

autor

Podsedek A.

autor

Wysokinska H.

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