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1999 | 41 |

Tytuł artykułu

In vitro pollination of excised ovaries

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
In vitro placental pollination can be employed to overcome barriers of self-incompatibility and cross-incompatibility. Pollen grains of self-incompatible plants are capable of germinating directly on ovules, and the whole process of sexual reproduction can be accomplished. In some combinations of crosses, direct pollination of ovules enables prezygotic incompatibility barriers to be overcome. As a result, hybrid embryos or even hybrid plants can develop, depending on the partners crossed. Pollen grains germinate abundantly on immature ovules irrespective of the stage of megasporogenesis or megagametogenesis, and their tubes can penetrate the integuments and the nucellus cells. Preliminary investigations revealed that pollen grains of seven selected species of gymnosperms germinated on ovules of various species of Angiospermae. Embryological analysis of ovules of Melandrium album fixed 3 days after pollination with pollen of Pinus wallihiana revealed the presence of remnants of pollen tubes and 2-celled embryos in the embryo sacs. Those preliminary observations have not been described heretofore.

Wydawca

-

Rocznik

Tom

41

Opis fizyczny

p.31-38,fig.

Twórcy

autor
  • A.Mickiewicz University, Al.Niepodleglosci 14, 61-713 Poznan, Poland

Bibliografia

  • Bhojwani SS, Raste AP. 1996. In vitro pollination and fertilization. In: Jain SM, Sopory SK, and Veilleux RE. [eds.], In vitro haploid production in higher plants, 237-262. Kluwer Academic Publishers, Amsterdam.
  • Dhaliwal S, King P. 1978. Direct pollination of Zea mays ovules in vitro with Z. mays, Z. mexicana and Sorghum bicolor pollen. Theoretical and Applied Genetics 53: 43-46.
  • De Verna JW, Myers JR, and Collins GB. 1987. Bypasing prefertilization barriers to hybridization in Nicotiana using in vitro pollination and fertilization. Theoretical and Applied Genetics 73: 665-671.
  • Dziedzic E, Lech W, Małodobry M, and Jankun A. 1998. Studies on fertilization of plum in vitro. Acta Horticulturae 478: 113-118.
  • Kameya T, and Hinata K. 1970. Test-tube fertilization of excised ovules in Brassica. Japanese Journal of Breeding 20: 253- 260.
  • Santa K, Rangaswamy WS, and Maheshwari P. 1962. Test-tube fertilization in a flowering plant. Nature 194: 1214-1217.
  • Leduc N, Douglas GC, and Monnier M. 1992. Methods for nonstigmatic pollination in Trifolium repens (Papilionaceae): seed set with self- and cross-pollination in vitro. Theoretical and Applied Genetics 83: 912-918.
  • Marubashi W, and Nakajima T. 1985. Overcoming cross-incompatibility between Nicotiana tabacum L. and N. rustica L. by test-tube pollination of ovule culture. Japanese Journal of Breeding 35: 429-437.
  • Rangaswamy NS. 1977. Application of in vitro pollination and in vitro fertilization. In: Reinert J, and Bajaj YPS [eds.], Plant Cell, Tissue and Organ Culture, 412-425. Springer-Verlag, Berlin.
  • Shehata A, and Zenkteler M. 1991. In vitro placental self and cross pollination in some species. Acta Societatis Botanicorum Poloniae 60: 93-99.
  • Sink KC, Power JB, and Natarello NY. 1978. The interspecific hybrid Petunia parodii and its relevance to somatic hybridization. Theoretical and Applied Genetics 53: 205-208.
  • Stewart JMcD. 1981. In vitro fertilization and embryo rescue. Environmental and Experimental Botany 21: 301-315.
  • Ślusarkiewicz-Jarzina A, and Zenkteler M. 1983. Development of hybrid plants from ovules of Nicotiana tabacum pollinated in vitro with pollen grains of Nicotiana knightiana. Experientia 39: 1399-1400.
  • Tarnowski MT, Shinkarena JK, and Larkina NJ. 1976. Production of interspecific tobacco hybrids by the pollination of ovules in vitro. Sovetskaja Genetika 12: 1209-1213. (English Translation).
  • Van Tuyl JM, Straathof TP, Bino RJ, and Kwakkenbos AAM. 1988. Effect of three pollination methods on embryo development and seed set in intra-and interspecific crosses between seven Lilium species. Sexual Plant Reproduction 1: 119-123.
  • Zenkteler M. 1967. Test tube fertilization of ovules of Melandrium album Mill, with pollen grains of several species of the Caryophylaceae family. Experientia 23: 775-777.
  • Zenkteler M. 1980. Intraovarian and in vitro pollination. International Review of Cytology 11B: 137-156.
  • Zenkteler M. 1990a. In vitro fertilization and wide hybridization in higher plants. Critical Review of Plant Science 9: 267- 279.
  • Zenkteler M. 1990b. In vitro fertilization of some species of Brassicaceae. Plant Breeding 105: 221-228.
  • Zenkteler M. 1991. Ovule culture and test-tube fertilization. Mededelingen van de Faculteit Landbouwwetenschappen Rijksuniversiteit Gent 56(4a): 1403-1410.
  • Zenkteler M, Melchers G. 1978. In vitro hybridization by sexual methods and by fusion of somatic protoplasts. Theoretical and Applied Genetics 52: 81-90.
  • Zenkteler M, Ślusarkiewicz-Jarzina A. 1986. Sexual reproduction in plants by applying the method of test tube fertilization of ovules. In: Horn W, Jensen CJ, Odenbach V, and Schieder O [eds.], Genetic Manipulation in Plant Breeding, 415-423. Walter de Gruyter, Berlin.
  • Zenkteler M, Maheshwaran G, and Williams EG. 1987. In vitro placental pollination in Brassica campestris and Brassica napus. Journal of Plant Physiology 128: 245-250.
  • Zenkteler M, Misiura E, and Guzowska J. 1975. Studies on obtaining hybrid embryos in test tubes. In: Mohan Ram HY, Shah JJ, and Shah CK [eds.], Form, Structure and Function in Plants, 180-187. Sarita Prakashan, Merrut, India.
  • Zenkteler M, Ślusarkiewicz-Jarzina A, and Woźna J. 1981. Cytological investigations of hybrid plants of Nicotiana alata Link and Nicotiana debneyi Domin, obtained by in vitro pollination of ovules. Bulletin de la Société des Amis des Sciences et des Lettres de Poznań Ser. D. 21: 79-83.

Typ dokumentu

Bibliografia

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