Adhesive properties of carcinoembryonic antigen glycoforms expressed in glycosylation-deficient Chinese hamster ovary cell lines

• Autorzy: Krop-Watorek A. , Klopocki A.G. , Czerwinski M. , Lisowska E.
• Języki publikacji: EN

Abstrakty

EN

Carcinoembryonic antigen (CEA) is an oncofoetal cell surface glycoprotein that serves as an important tumour marker for colorectal and some other carcinomas. Its immunoglobulin-like structure places CEA within the immunoglobulin superfamily. CEA functions in several biological roles including homotypic and heterotypic (with other CEA family members) cell adhesion. Cell-cell interaction can be modulated by different factors, e.g., post-translational modifications such as glycosylation. The pur­pose of this study was to examine whether changes in carbohydrate composition of CEA oligosaccharides can influence homotypic (CEA-CEA) interactions. In order to modulate glycosylation of CEA we used two different glycosylation mutants of Chi­nese hamster ovary (CHO) cells, Lec2 and Lec8. Lec2 cells should produce CEA with nonsialylated N-glycans, while Lec8 cells should yield more truncated sugar struc­tures than Lec2. Parental CHO (Pro5) cells and the glycosylation deficient mutants were stably transfected with CEA cDNA. All three CEA glycoforms, tested in a solid-phase cell adhesion assay, showed an ability to mediate CEA-dependent cell ad­hesion, and no qualitative differences in the adhesion between the glycoforms were observed. Thus, it may be assumed that carbohydrates do not play a role in homotypic adhesion, and the interactions between CEA molecules depend solely on the poly- peptide structure.

Słowa kluczowe

EN

cell line; carcinoembryonic antigen; carcinoma; Chinese chamster; glycosylation defective mutant; tumour marker; N-glycoform; chamster

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 1
• Opis fizyczny: p.273-283,fig.

Twórcy

autor

Krop-Watorek A.

• Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wroclaw, Poland

autor

Klopocki A.G.

autor

Czerwinski M.

autor

Lisowska E.

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