Haploidy in early bovine embryos produced in vitro

• Autorzy: Lechniak D
• Języki publikacji: EN

Abstrakty

EN

Early bovine embryos (two - to sixteen blastomeres) produced in vitro were cytogenetically analysed to determine the incidence of haploidy. Follicular oocytes were matured in vitro and inseminated with sperm prepared using the swim up method. After 2-3 days of culture, chromosome slides were prepared according to the air-drying technique and stained with Giemsa. Altogether 202 embryos produced metaphase spreads. Of these 4.5% were pure haploid embryos and 5.5% displayed a haploid cell line within mosaic embryos (n/2n and n/3n). The occurrence of haploid embryos observed in this study was compared to results of other studies and a possible origin of haploidy was discussed.

Słowa kluczowe

EN

fertilization; haploidy; bovine embryo; in vitro; mixoploidy

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1995
• Tom: 36
• Numer: 4
• Opis fizyczny: p.363-371,fig.

Twórcy

autor

Lechniak D

• Agricultural University of Poznan, 60-637 Poznan, Wolynska 33, Poland

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