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2008 | 13 | 1 |

Tytuł artykułu

The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
We cultured calnexin-disrupted and wild-type Saccharomyces cerevisiae strains under conditions of heat stress. The growth rate of the calnexin-disrupted yeast was almost the same as that of the wild-type yeast under those conditions. However, the induced mRNA level of the molecular chaperone PDI in the ER was clearly higher in calnexin-disrupted S. cerevisiae relative to the wild type at 37°C, despite being almost the same in the two strains under normal conditions. The western blotting analysis for PDI protein expression in the ER yielded results that show a parallel in their mRNA levels in the two strains. We suggest that PDI may interact with calnexin under heat stress conditions, and that the induction of PDI in the ER can recover part of the function of calnexin in calnexin-disrupted yeast, and result in the same growth rate as in wild-type yeast.

Wydawca

-

Rocznik

Tom

13

Numer

1

Opis fizyczny

p.38-48,fig.,ref.

Twórcy

autor
  • Liaoning University, Shenyang 110036, China
autor
autor
autor
autor

Bibliografia

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  • 6. Jackson, M.R., Cohen-Doyle, M.F., Peterson, P, A. and Williams, D.B. Regulation of MHC class I transport by the molecular chaperone, calnexin (p88, IP90). Science 263 (1994) 384-387.
  • 7. Ware, F.E., Vassilakos, A., Peterson, P.A., Jackson, M.R., Lehrman, M.A. and Williams, D.B. The molecular chaperone calnexin binds Glc1Man9GlcNAc2 oligosaccharide as an initial step in recognizing unfolded glycoproteins. J. Biol. Chem. 270 (1995) 4697-4704.
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  • 9. Jakob, C.A., Burda, P. S., te Heesen, S., Aebi, M. and Roth, J. Genetic tailoring of N-linked oligosaccharides: the role of glucose residues in glycoprotein processing of Saccharomyces cerevisiae in vivo. Glycobiology 8 (1998) 155-164.
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  • 11. Song. Y., Sata, J., Saito, A., Usui, M., Azakami, H. and Akio, K. Effects of calnexin deletion in Saccharomyces cerevisiae on the secretion of glycosylated lysozymes. J. Biochem. 130 (2001) 757-764.
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  • 23. Arima, H., Kinoshita, T., Ibrahim H.R., Azakami, H. and Kato, A. Enhanced secretion of hydrophobic peptide fused lysozyme by the introduction of N-glycosylation signal and the disruption of calnexin gene in Saccharomyces cerevisiae. FEBS Lett. 440 (1998) 89-92.
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Typ dokumentu

Bibliografia

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Identyfikator YADDA

bwmeta1.element.agro-article-465152c7-4560-489a-8cdd-a77059a9f85c
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