Antioxidative effect of plant extracts and flavones on liposome and erythrocyte membranes

• Autorzy: Gabrielska J. , Korzeniowska M. , Wojdylo A.

Warianty tytułu

PL

Dzialanie antyoksydacyjne ekstraktow roslinnych i flawonow na blony liposomow i erytrocytow

• Języki publikacji: EN

Abstrakty

EN

The paper describes the use of liposomes and erythrocytes membrane as a real membrane models to evaluate the potential benefits of several plants extracts and two flavones in relation to lipid peroxidation. The antioxidant behaviour of the plant extracts from pine (Pinus silvestris L), hawthorn (Crataegus oxyacantha L, two extracts: from hawthorn’s leaves-l and bark-b), evening primrose (Oenothena paradoxa Hudziok – three extracts differ in procyanidins content P1, P2 and P3) and rosemary (Rosmarinus officinalis, as a standard for extracts) and flavones of baicalin and rutin have been studied. The results obtained showed that the studied extracts exhibited differentiated, dose-dependent antioxidant activity against phospatidylcholine liposomes (rosemary>pine≈hawthorn-l>hawthorn-b≈P1≈P2≈P3; statistically significant differences were observed between the extracts at p≤0.05) and erythrocyte membranes (rosemary≥hawthorn-b≈hawthorn-l>P1≈pine>P2≈P3) when the oxidation was induced by UV-C radiation. They also reduce the oxidation of liposomes and erythrocyte membrane when its oxidation was induced by 2,2’-azobis(2-amidinopropane) dihydrochloride (pine≥P1≈P2≈rosemary≈P3 in the case of liposomes and rosemary>> pine≥P1≈P2≥P3 in a case of erythrocyte). Moreover, the results of the study show that baicalin is characterised by high inhibition ability towards liposome PC peroxidation, as well as towards erythrocyte ghosts, when oxidation was initiated by UV radiation. However, at the same experimental conditions, the inhibitory capacity of rutin was about 7-8 times weaker. The presence of cholesterol in liposome membrane decreased the level of membrane peroxidation but do not influenced on the antioxidant activity of hawthorn extract.

PL

W pracy zastosowano liposomy oraz błony erytrocytów jako modele błon biologicznych do opisu procesu utleniania lipidów. Zbadano antyoksydacyjne działanie ekstraktów roślinnych z sosny (Pinus silvestris L), głogu (Crataegus oxyacantha L,dwa ekstrakty: z liści i kory głogu), wiesiołka (Oenothena paradoxa Hudziok, trzy ekstrakty różniące się zawartością procyjanidyn oznaczone P1, P2 and P3) and rozmarynu (Rosmarinus officinalis, jako standardu dla ekstraktów) oraz flawony bajkaliny i rutyny. Uzyskane wyniki badań wykazały zależną od stężenia, zróżnicowaną aktywność antyoksydacyjną ekstraktów w stosunku do błon liposomów (rozmaryn>sosna≈głóg-l>głóg-b≈P1≈P2≈P3; oraz błon erytrocytów (rozmaryn≥głóg-b≈głóg-l>P1≈sosna>P2≈P3) w sytuacji gdy utlenianie indukowano promieniowaniem UV-C. Redukują one także utlenienie liposomów i błon erytrocytów indukowane 2,2’-azobis(2-amidinopropane)dihydrochloride (AAPH) (sosna≥P1≈P2≈rozmaryn≈P3, w przypadku liposomów and rozmaryn>>sosna≥P1≈P2≥P3, w przypadku erytrocytów). Bajkalina posiada wysoką zdolność inhibicji peroksydacji zarówno liposomów PC jak i cieni erytrocytów w sytuacji utleniania indukowango promieniami UV, natomiast rutyna w tych warunkach jest ok. 7-8 krotnie słabszym inhibitorem procesu. Obecność cholesterolu w błonach liposomów w zasadzie zmniejsza poziom peroksydacji liposomów (różnice istotne statystycznie) lecz nie wpływa na aktywność antyoksydacyjną ekstraktu z liści głogu.

Słowa kluczowe

EN

liposome membrane; erythrocyte membrane; flavone; plant extract; antioxidant effect

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2007
• Tom: 57
• Numer: 4[A]
• Opis fizyczny: p.145-150,fig.,ref.

Twórcy

autor

Gabrielska J.

• Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-375 Wroclaw, Poland

autor

Korzeniowska M.

autor

Wojdylo A.

Bibliografia

• 1. Arts M.J.T.J., Sabastiaan D.J., Voss H-P., Haenen G.R.M.M., Bast A., A new approach to assess the total antioxidant capacity using the TCAE assay. Food Chem., 2004, 88, 567-570.
• 2. Basaga H., Tekkaya C., Acikel F., Anioxidative and free radical scavenging properties of rosemary extract. Lebens.-Wiss. uTechnol., 1997, 30, 105-108.
• 3. Bonina F., Lanza M., Montenegro L., Puglis C., Tomaino A., Trombetta D., Castelli F., Saija A., Flavonoids as potential protective agents photo-oxidative skin damage. Int. J. Pharmac., 1996, 145, 87-94.
• 4. Bose B., Chatterjee S.N., Correlation between UVA-induced changes in microviscosity, permeability and malondialdehyde formation in liposomal membrane. J. Photochem. Photobiol. B: Biology, 1995, 28, 149-153.
• 5. Buege J.A., Aust S.D., Microsomal lipid peroxidation. Methods Enzymol., 1978, 52c, 302-310.
• 6. Christen Y., Oxidative stress and Alzheimer disease. Am. J. Clin. Nutr., 2000, 7, S621-629.
• 7. Dodge J.T., Mitchel C., Hanahan D.J., The preparation and biochemical characteristics of haemoglobin – free gosts of erythocytes. Arch. Biochem. Biophys., 1963, 100, 119-130.
• 8. Duffy C.F., Power R.F., Antioxidant and antimicrobial properties of some Chinese plant extracts. Int. J. Antimic. Agent, 2001, 17, 527-529.
• 9. Foti M., Piattelli M., Baratta M.T., Ruberto G., Flavonoids, coumarins, and cinnamic acids as antioxidants in micellar system. Structure-activity relationship. J. Agric. Food Chem., 1996, 44, 497-501.
• 10. Frankel E.N., Huang S.W., Aeschbach R., Prior E., Antioxidant activity of a rosemary extract and its constituents, carnosis acid, carnosol, and rosmarinic acid in bulk oil and oil-in water emulsions. J. Agric. Food Chemistry, 1996, 44, 131-135.
• 11. Gabrielska J., Oszmiański J., Antioxidant activity of anthocyanin glycoside derivatives evaluated by inhibition of liposome oxidation. Z. Naturforch., 2005, 60c, 399-407.
• 12. Gabrielska J., Soczyńska-Kordala M., Przestalski S., Antioxidative effect of kaempferol and its equimolar mixture with phenyltin compounds on UV-irradiated liposome membranes. J. Agric. Food Chem., 2005, 69, 9-14.
• 13. Hertog MGL., Kromhout D., Aravanis C., Blackburn H., Buzina R., Fidanza F., Giampaoli S., Jensen A., Menotti A., Nedeljkovic S., Pekkarinnen M., Simic BS., Toshima H., Feskens E.J.M., Holman P.C.H., Katan M.B., Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study. Arch. Intern. Med., 1995, 155, 381-386.
• 14. Huang D., Ou B., Prior R.L., The chemistry behind antioxidant capacity assays. J. Agric. Food Chem., 2005, 53, 1841-1856.
• 15. Jellinger K.A., The role of iron in neurodegeneration. Prospects for pharmacotherapy of Parkinsons-disease. Drugs Aging, 1999, 14, 115-140.
• 16. Kamei H., Hasimoto Y., Koide T., Kojima T., Hasegawa M., Ani-tumor effect of methanol extracts from red and white wines. Cancer Biother. Radiopharm., 1998, 13, 447-452.
• 17. Kruk I., The Handbuch of Environmental Chemistry, Reaction and Processes. Ed. O. Hutrzinger, Springer-Verlag Berlin Heidelberg, 1998, 2, pp. 167-228.
• 18. Kupfer R., Dwyer-Nield L.D., Malkinson A.M., Thompson J.A., Lung toxicity and tumor promotion by hydroxylated derivatives of 2.6-di-tert-butyl-4-methylphenol (BHT) and 2-tert-butyl-4 -methyl-6-iso-propylphenol: correlation with quinine methide reactivity. Chem. Res. Toxicol., 2002, 8, 1106-1112.
• 19. Lingberg-Madsen H., Bertelsen G., Species as antioxidants. Trends Food Sci. Technol..1995, 6, 271-274.
• 20. Malterud K.E., Diep O.H., Sund R.B., C-Methylated dihydrohalcones from Mirica gale L: effect as antioxidants and as scavengers of 1.1-diphenyl-2-picrylhydrazyl. Pharmacol. Toxicol., 1996, 78, 111-116.
• 21. Mc Vean M., Liebler D.C., Inhibition of UVB induce DNA photodamage in mouse epidermis by topically applied (-tocopherol. Carcinogenesis, 1997, 18, 1617-1622.
• 22. Ohkawa H., Ohishi N., Yagi K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 1979, 95, 351-358.
• 23. Osawa T., Protective role of rice polyphenols in oxidative stress. Anticancer Res., 1999, 19, 3645-3650.
• 24. Oszmiański J., Bourzeix M., Preparation of catechin and procyanidin standards from hawthorn (Crategus azorus L) and pine (Pinus mesogeensis Fieschi) barks. Pol. J. Food Nutr. Sci., 1995, 45, 91-98.
• 25. Parasassi T., Giusti A.M., Raimondi M., Ravagnan G., Sapora O., Gratton E., Cholesterol protects the phospholipid bilayer from oxidative damage. Free Rad. Biol. Med., 1995, 19, 511-516.
• 26. Peres-Fons L., Aranda F.J., Guillen J., Villalain J., Micol V., Rosemary (Rosmarinus officinalis) diterpenes affect lipid polymorphism and fluidity in phospholipid membranes. 2006, 453, 224-236.
• 27. Peterson J., Dwyer M., Flavonoids: dietary occurance and biochemical activity. Nutr. Res., 1998, 18, 1995-2018.
• 28. Piotrowski J.J., Hunter G.C., Eskelsen C.D., Dubik M.A., Bernhard V.M., Evidence for lipid peroxidation in atherosclerosis. Life Sci., 1990, 46, 715-721.
• 29. Rice-Evans C.A., Miller N.J., Paganga G., Structure-antioxidant activity of flavonoids and phenolic acids. Free Rad. Biol. Med., 1996, 20, 933-956.
• 30. Samaniego S.C., Troncoso G.A.M., Garcia-Parrilla M.C., Quesada G.J.J., Lopes Garcia de la Serrana. H., Lopez Martinez M.C., Different radical scavenging tests in virgin olive oil and their relation to the total phenol content. Anal. Chim. Acta, 2007, 597, 103-107.
• 31. Sastre J., Pallardo F.V., Vina J., Mitochondrial oxidative stress plays a key role in aging and apoptosis. IUBMB Life, 2000, 49, 427-435.
• 32. Saucier C.T., Waterhouse A.L., Synergetic activity of catechin and other antioxidants. J. Agric. Food Chem..1999, 47, 4491‑4494.
• 33. Skupień K., Oszmianski J., Kostrzewa-Nowak D., Tarasiuk J., In vitro antileukeamic activity of extracts from berry plant leaves against sensitive and multidrug resistant HL60 cells. Cancer Lett., 2006, 236, 282-291.
• 34. Sokół-Łętowska A., Oszmiański J., Wojdyło A., Antioxidant activity of the phenolic compounds of hawthorn, pine and skullcap. Food Chem., 2007, 103, 853-859.
• 35. Wiseman H., Halliwel B., Damage to DNA by reactive oxygen and nitrogen species: role in inflammatory disease and progression to cancer. Biochem. J., 1996, 313, 17-29.
• 36. Yang M.H., Schaich K.M., Factors affecting DNA damage caused by lipid hydroperoxides and aldehydes. Free Radical Biol. Med., 1996, 20, 225-236.
• 37. Zandi P., Gordon M.H., Antioxidant activity of extracts from old tea leaves. Food Chem., 1999, 64, 285-288.