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2007 | 10 | 3 |

Tytuł artykułu

Fertilizing capacity of boar semen frozen in an extender supplemented with ostrich egg yolk lipoprotein fractions - A pilot study

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
A limited field trial was performed to evaluate the fertilizing capacity of boar spermatozoa frozen in an extender supplemented with lipoprotein fractions isolated from ostrich egg yolk (LPFo). Boar semen, diluted in an extender containing lactose with lyophilized lipoprotein fractions, glycerol and Orvus Es Paste (lactose-LPFo-G), was frozen using a controlled programmable freezer. Sperm characteristics, such as motility, plasma membrane and acrosome integrity, and mitochondrial function were monitored. Post-cervical artificial inseminations (post-CAIs) in multiparous sows (Polish Large White) were performed using the Soft & Quick® catheter/cannula set. Sows were inseminated 2 to 3 times within one oestrus. Possible returns of sows to oestrus were determined from 21 to 30 days after post-CAIs. In this field trial, sows inseminated with 2 x 109 motile frozen-thawed spermatozoa resulted in pregnancy and farrowing rates of 75%, respectively. The average piglets born live was 10.5 ± 0.4 (mean ± SEM). The data of this study showed that post-CAI of boar semen frozen in LPFo-containing extender has the potential to provide acceptable fertility results. Further investigations are needed to elucidate the cause of variations in pregnancy/farrowing rate associated with frozen-thawed boar semen.

Wydawca

-

Rocznik

Tom

10

Numer

3

Opis fizyczny

p.131-135,ref.

Twórcy

autor
  • University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland
autor
autor

Bibliografia

  • Bolarín A, Roca J, Rodríguez-Martíinez H, Hernández M, Vázquez JM, Martínez E (2006) Dissimilarities in sow’s ovarian status at the insemination time could explain dif­ferences in fertility between farms when frozen-thawed semen is used. Theriogenology 65: 669-680.
  • Erikson BM, Petersson H, Rodriguez-Martinez H (2002) Field fertility with exported boar semen frozen in the new FlatPack container. Theriogenology 58: 1065-1079.
  • Evenson DP, Wixon R (2006) Clinical aspects of sperm DNA fragmentation detection and male infertility. Theriogenology 65: 979-991.
  • Fraser L, Lecewicz M, Strzezek J (2002) Fluorometric as­sessments of viability and mitochondrial status of boar spermatozoa following liquid storage. Pol J Vet Sci 5: 85-92.
  • Fraser L, Strzezek J (2004) The use of the comet assay to assess DNA integrity of boar spermatozoa following liquid preservation at 5°C and 16°C. Folia Histochem Cytobiol 42: 49-55.
  • Garner DL, Johnson LA (1995) Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol Reprod 53: 276-284.
  • Johnson LA, Weitze KF, Fiser P, Maxwell WM (2000) Stor­age of boar semen. Anim Reprod Sci 62: 143-172.
  • Lecewicz M, Fraser L, Strzezek J (2004) Intra-cervical in­semination in sows. Hodowca Trzody Chlewnej 2: 18-19 (in Polish).
  • Martínez EA, Vázquez JM, Parrilla I, Cuello C, Gil MA, Rodríguez-Martíinez H, Roca J, Vázquez JL (2006) Inci­dence of unilateral fertilizations after low dose deep in­trauterine insemination in spontaneously ovulating sows under field conditions. Reprod Dom Anim 41: 41- 47.
  • Martínez EA, Vázquez JM, Roca J, Cuello C, Gil MA, Par­rilla I, Vázquez JL (2005) An update on reproductive technologies with potential short-term application in pig production. Reprod Dom Anim 40: 300-309.
  • Roca J, Carvajal G, Lucas X, Vazquez JM, Martinez EA (2003) Fertility of weaned sows after deep intrauterine insemination with a reduced number of frozen-thawed spermatozoa. Theriogenology 60, 77-87.
  • Roca J, Vazquez JM, Gil MA, Cuello C, Parrilla I, Martinez EA (2006) Challenges in pig artificial insemination. Rep­rod Dom Anim 41: 43-53.
  • Rodriguez-Martinez H (2006) Can we increase the estimate value of semen assessment? Reprod Dom Anim 41: 2-10.
  • Sakkas D, Mariethoz E, Manicardi G, Bizzaro D, Bianchi PG, Bianchi U (1999). Origin of DNA damage in ejaculated human spermatozoa. Rev Reprod 4: 31- 37.
  • Strzeżek J (1998) Actual problems relating to artificial in­semination in sows - factors influencing its effectiveness. Zeszyty Naukowe Przeglądu Hodowlanego 99: 49-73 (in Polish).
  • Strzeżek J (2002) Technological progress in insemination of sows. Wieś Jutra 1: 12-14 (in Polish).
  • Strzeżek J, Fraser L, Dziekońska A Lecewicz M (2006) Complexity of technology preservation of boar semen - fundamental and applicative approaches. Reprod Dom Anim 41: 325.
  • Strzeżek J, Głogowski J, Hopfer E, Wojtkiewicz K (1985) The “Kortowska” method of freezing boar semen. Med Weter 41, 349-353.
  • Strzeżek J, Lecewicz M, Dziekońska A, Fraser L (2005) A simple method of extraction of lipoprotein fractions from avian egg yolk - protective effect on cooled boar semen. Theriogenology 6: 496-497.
  • Waberski D, Weitze F, Gleumes T, Schwartz M, Willmen T, Petzoldt R (1994) Effects of time of insemination rela­tive to ovulation on fertility with liquid and frozen semen. Theriogenology 42: 831-840.
  • Watson PF (1975) Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Vet Rec 97: 12-15.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-44e0dd8a-4be9-492c-ab3a-a4727192f207
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