Recommended method for recovery of Toxocara and other geohelminth eggs from soil

• Autorzy: Mizgajska-Wiktor H
• Języki publikacji: EN

Abstrakty

EN

The flotation method elaborated for recovery of Toxocara and other geohelminth eggs from soil is described. Soil samples of about 500 ml volume are picked from 3-cm superficial layer of the ground. In the laboratory, 40 g of dry and sifted material is analysed according to following procedure: 1 h standing, 20 minutes shaking and 3 minutes centrifugation (1500 rpm) in 5% sodium hydroxide (NaOH), then centrifugation, like above, with H₂O for washing the sample and next with the saturated sodium nitrate (NaNO₃) for flotation the eggs. Specimen is prepared by placing a cover slip on the positive meniscus of the flotation liquid.

Słowa kluczowe

EN

flotation; soil; recovery; geohelminth; method; Toxocara; egg

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2005
• Tom: 51
• Numer: 1
• Opis fizyczny: p.21-22,fig.,ref.

Twórcy

autor

Mizgajska-Wiktor H

• Eugeniusz Piasecki School of Physical Education, Krolowej Jadwigi 27/39, 61-871 Poznan, Poland

Bibliografia

• [1] Spindler L.A. 1929. On the use of method for the isolation of Ascaris eggs from soil. Annals of the Journal of Hygiene 10: 157-163.
• [2] Dada B.J.O. 1979. A new technique for the recovery of Toxocara eggs from soil. Journal of Helminthology 53: 141-144.
• [3] Quinn R., Smith H.V., Girdwood R.W.A. 1980. Studies on the incidence of Toxocaris spp. ova in the environment. 1. A comparison of flotation procedures for recovery of Toxocara spp. ova from soil. Journal of Hygiene, Cambridge University 84: 83-89.
• [4] Nunes C.M., Sinhorini J.L., Ogassawara S. 1994. Influence of soil texture in the recovery of Toxocara canis eggs by flotation method. Veterinary Parasitology 53: 269-274.
• [5] Mizgajska H. 2001. Eggs of Toxocara spp in the environment and their public health implication. Journal of Helminthology 75: 147-151.