Detection and differentiation of Escherichia coli populations from human, animal and avian feces, and different water sources

• Autorzy: Abdallah S A
• Języki publikacji: EN

Abstrakty

EN

A total of 138 E. coli isolates obtained from fecal samples of various mammals and birds and water samples of different origin were compared. The comparison included multiple antibiotic resistance to a total of 21 antibiotics, the protein profile (PAGE) of the isolates, the DNA fragments of each isolate after treatment with the restriction enzymes, HindIII, EcoRI, EcoRV, BamHI and BglII and the presence of plasmid(s). Our results indicated that human, horse and Nile water isolates were resistant to 62% of the antibiotics used, while cat isolates were resistant to 47%, mouse isolates 72%, avian to 34%, Ismailia canal 67%, household water reservoir 81% and swimming pool isolates were resistant to 57%. Protein profile (PAGE) analysis is the second method used; the protein with 20.29 kd molecular weight is common in avian, household water reservoir and swimming pool isolates. The third method includes DNA fragments using different restriction enzymes HindIII, EcoRI, EcoRV, BamHI and BglII.

Słowa kluczowe

EN

fingerprinting; animal; water; feces; bird; fecal contamination; man; fecal pollution; Escherichia coli; water resource; detection; water source; differentiation

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2005
• Tom: 14
• Numer: 5
• Opis fizyczny: p.639-646,fig.,ref.

Twórcy

autor

Abdallah S A

• Science and Education Ain Shams University, Cairo, Egypt

Bibliografia

• 1. FLEISHER J. M., KAY D., SALMEN R. I., JONES F., WYER M. D., GODFREE A. F. Marine waters, contaminated with domestic sewage: nonenteric illnesses associated with bather exposure in the United Kingdom. Am. J. Public Health. 86, 1228, 1996.
• 2. SAUER T. J., DANIEL T. C., NICHOLS D. J., WEST C. P., MOORE JR. P. A., WHEELER G. L. Runoff water quality from poultry litter –treated pasture and forest sites. J. Environ. Qual. 29, 515, 2000.
• 3. U. S. ENVIRONMENTAL PROTECTION AGENCY. TMDL tracking system data, version 1.0. Total maximum daily load program. U. S. Environmental Protection Agency Office of Water, Washington, D. C.[Online] http://www. epa.gov/owow/tmd/trcksys.html, 1998.
• 4. JONES I. G., ROWORTH M. An outbreak of Escherichia coli 0157 and campylobacteriosis associated with contamination of a drinking water supply. Public Health (London) 110, 277, 1996.
• 5. GUGNANI H. C. Some emerging food and water borne pathogens, J. Commun. Dis. 31, 65, 1999.
• 6. BERROUANE Y. F, MCNUTT I. A., BUSCHELMAN B. J., RHOMBERG P. R., SANFORD M. D., HOLLIS R. J., PFALLER M. A., HERWALDT L. A. Outbreak of severe Pseudomonas aeruginosa infections caused by a contaminated drain in a whirlpool bathtub. Clin. Infect. Dis. 31, 1331, 2000.
• 7. LICENCE K., OATES K. R., SYNGE B. A., REID T. M. An outbreak of E. coli 0157 infection with evidence of spread from animals to man through contamination of a private water supply. Epidemiol. Infect. 126, 135, 2001.
• 8. PUCCH M. C., MCANULTY J. M., LESJAK M., SHAW N., HERON L., WATSON J. M. A statewide outbreak of cryptosporidiosis in new south Wales associated with swimming at public pools. Epidemiol. Infect. 126, 389, 2001.
• 9. KRUMPERMAN P. H. Multiple antibiotic resistamce indexing of Escherichia coli to identify high risk sources of fecal contamination of foods. Appl. Envirom. Microbiol. 46, 165, 1983.
• 10. GUAN S., XU R., CHEN S., ODUMERU J., GYLES C. Development of a procedure for discriminating among Escherichia coli isolates from animal and human sources. Appl. Environ. Microbiol. 68 (6), 2690, 2002.
• 11. PARVEEN S., MURPHREE R. L., EDMISTON L., KASPAR C. W., PORTIER K. M., TAMPLIN M. L. Association of multiple- antibiotic – resistance profiles with point and non point sources of Escherichia coli in Apalachicola Bay. Appl. Environ. Microbiol. 63, 2607, 1997.
• 12. HARWOOD V. J., WHITLOCK J., WITHINGTON V. Classification of antibiotic resistance patterns of indicator bacteria by discriminant analysis: use in predicting the source of fecal contamination in subtropical water. Appl. Environ. Microbiol. 66, 3698, 2000.
• 13. PARVEEN S., PORTIER K. M., ROBINSON K., EDMISTON L., TAMPLIN M. L. Discriminant analysis of ribotype profiles of Escherichia coli for differentiating human and nonhuman sources of fecal pollution. Appl. Environ. Microbiol. 65, 3142, 1999.
• 14. CARSON A. C., SHEAR B. L., ELLERSIECK M. R., ASFAW A. Identification of fecal Escherichia coli from humans and animals by ribotyping. Appl. Environ. Microbiol. 6-7 (4), 1503, 2001.
• 15. KARIUKI S., GILKS C., KIMARI J., OBANDAA., MUYODI J., WAIYAKI P., HART C. A. Genotype analysis to Escherichia coli strains isolated from children and chickens living in close contact. Appl. Envirom. Microbiol. 65, 472, 1999.
• 16. DOMBEK P. F., JOHNSON I. K., ZIMMERLEY S. J., SADOWSKY M. J. Use of repetitive DNA Sequences and PCR to differentiate Escherichia coli isolates from human and animal sources. Appl. Environ. Microbiol. 66, 2572, 2000.
• 17. BERNHARD A. E., FIELD K. G. A PCR assay to discriminate human and animal feces on the basis of host differences in Bacteroides –Prevotella genes encoding 16srRNA. Appl. Environ. Microbiol. 66, 4571, 2000.
• 18. DIFCO MANUAL (10th Ed.) Dehydrated culture media reagents for microbiol, DIFCO Laboratories. Detroit Michigan 48232 USA, 1994.
• 19. AMERICAN PUBLIC HEALTH ASSOCIATION (APHA). Standard methods for the examination of water and waste water. American Public Health Association, Washington, D. C. 1995.
• 20. GREENWOOD D. Antibiotics and chemotherapeutic agents used in the therapy of bacterial infection. In: Toply and Wilson’s Microbiology and Microbial Infections. Balows A. and Duerden B. I.(eds). volume (2), Systematic Bacteriology Ninth edn. Oxford University press, Inc. New York. pp. 195-229, 1998.
• 21. GALLAGHER S., SMITH J. A. One dimentional gel electrophoresis of protein In current protocol in molecular biology (Ausubel et al., eds.) unit 10.2. Greene Publishing and Wiley – Interscience, New York. 1995.
• 22. MANIATIS T., FRITSCH E. F., SAMBROOK J. Molecular cloning. A laboratory manual. Publisher: Coldspring Harbor Laboratory. 1982.
• 23. WIGGINS B. A. Discriminant analysis of antibiotic resistance patterns in fecal streptococci, a method to differentiate human and animal sources of fecal pollution in natural waters. Appl. Envirom. Microbiol. 62, 3997, 1996.
• 24. HAGEDORN C., ROBINSON S. L., FILTZ J. R., GRUBBS S. M., ANGIER T. A., RENCAU R. B. Determining sources of fecal pollution in a rural Virginia watershed with antibiotic resistance patterns in fecal streptococci. Appl. Environ. Micorbiol. 65, 5522, 1999.
• 25. KASPAR C. W., BURGESS J. L., KNIGHT I. T., COLWELL R. R. Antibiotic resistance indexing of Escherichia coli to identify sources of fecal contamination in water. Can. J. Microbiol. 36, 891, 1990.
• 26. VALSANGIACOMA C., BAGGI F., GAIA V., BALMELLI T., PEDUZZI R., PIFFARETTI J. C. Use of amplified fragment length polymorphism in molecular typing of Legionella pneumophila and application to epidemiological studies. J. Clin. Microbiol. 33, 1716, 1995.
• 27. DESAI M., THRELFALL E. J., STANLEY J. Fluorescent amplified Fragment length polymorphism subtyping of the Salmonella enterica serovar Enteritidis phage type 4 clone complex. J. Clin. Microbiol. 39, 201, 2001.