In vitro selection and characterization of Ni-tolerant callus lines of Setaria italica L.

• Autorzy: Rout G R , Samantaray S. , Das P.
• Języki publikacji: EN

Abstrakty

EN

Nickel tolerant callus lines of Setaria italica L. were developed from callus cultures grown on MS medium supplemented with 0.5 mg·dm⁻³ kinetin+2.0 mg·dm⁻³ 2,4-D+2.0 mg·dm⁻³ Ni⁺². Standard growth parameters such as callus fresh and dry weight, growth tolerance index were used as indicators of nickel toxicity. Measurements as early as 2 weeks after the beginning of the treatments did not yield consistent results. However, growth tolerance index at 4, and 8 weeks after the beginning of treatments yielded significant differences among the non-tolerant and tolerant calli. The tolerant calli has enhanced growth at 2.0 mg·dm⁻³ Ni⁺² while non-tolerant calli showed a reverse trend in growth in the presence of 2.0–2.5 mg·dm⁻³ of nickel. The tolerant calli differentiated into mass of embryogenic calli within 4 weeks of culture which could be maintained for prolonged period without loss of regenerative capacity.

Słowa kluczowe

EN

selection; metal tolerance; callus culture; Setaria italica; in vitro; nickel

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1998
• Tom: 20
• Numer: 3
• Opis fizyczny: p.269-275,fig.

Twórcy

autor

Rout G R

• Regional Plant Resource Centre, Bhubaneswar-751015, Orissa, India

autor

Samantaray S.

autor

Das P.

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