Transcriptomic 'portraits' of canine mammary cancer cell lines with various phenotypes

• Autorzy: Krol M , Pawlowski K M , Skierski J , Turowski P , Majewska A , Polanska J , Ugorski M , Morty R E , Motyl T
• Języki publikacji: EN

Abstrakty

EN

In light of the high incidence of mammary cancer in dogs and completion of the canine genome sequencing, the new possibilities of gene profiling by using DNA microarrays give hope to veterinary oncology. The cell lines isolated from mammary tumors are a valuable tool in developing and testing new pathway-specific cancer therapeutics. Differential cytometric analysis of 6 canine mammary cancer cell lines was performed. We divided cell lines into 3 groups based on their phenotype: 2 lines with high proliferative potential, 2 lines with high antiapoptotic potential, and 2 lines with high metastatic potential. DNA microarray analysis revealed common genes for cell lines of each group. We found that genes encoding the receptors for growth hormone and ghrelin are related to high proliferation rate, while ABR (active BCR-related) and TMD1 (TM2 domain containing 1) genes are related to a high antiapoptotic potential of the cancer cells. Metastatic properties of mammary cancer cells seem to be associated with elevated expression of PGP (P glycoprotein), SEMA3B (semaphorin 3B), and STIM1 (stromal interaction molecule 1).

Słowa kluczowe

PL

canine mammary cancer cell; DNA microarray; cell line; phenotype; transcriptome; dog; mammary cancer; veterinary oncology

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2010
• Tom: 51
• Numer: 2
• Opis fizyczny: p.169-183,fig.,ref.

Twórcy

autor

Krol M

• Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences [SGGW], Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Pawlowski K M

• Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences [SGGW], Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Skierski J

• Cell Biology Department, National Medicines Institute, Warsaw, Poland

autor

Turowski P

• Department of Internal Medicine, University of Giessen Lung Center, Justus Liebig University, Giessen, Germany

autor

Majewska A

• Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences [SGGW], Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Polanska J

• Department and Clinic of Obstetrics, Ruminant Diseases and Animal Health Care, Wrocław University of Environmental and Life Sciences, Wroclaw, Poland

autor

Ugorski M

• Department of Biochemistry, Pharmacology and Toxicology, Wrocław University of Environmental and Life Science, Wrocław, Poland
• Department Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wrocław, Poland

autor

Morty R E

• Department of Internal Medicine, University of Giessen Lung Center, Justus Liebig University, Giessen, Germany

autor

Motyl T

• Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences [SGGW], Nowoursynowska 159, 02-776 Warsaw, Poland

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