Flow cytometric estimation of ploidy in anisoploid sugar-beet populations

• Autorzy: Sliwinska E , Steen P.
• Języki publikacji: EN

Abstrakty

EN

Determination of ploidy level is a common practice in sugar-beet breeding and seed production. Lately many laboratories have replaced microscopic chromosome counting by flow cytometric estimation of the nuclear DNA content. The possibility of direct estimation of cytological composition in anisoploid populations instead of individual plant analyses was investigated. Diploid, triploid and tetraploid sugar-beet lines as well as their mixtures were tested. The results obtained show that the suggested method is fast, simple and, after a proper transformation of the computer values, could be applied in breeding and seed production of polyploid crops. Precise estimation of contamination by small admixture of seeds or plants of undesirable ploidy, however, requires individual plant rather than population analyses.

Słowa kluczowe

EN

production; Beta vulgaris; poliploid cultivar; ploidy determination; nuclear DNA; flow cytometry; DNA; seed; sugar-beet

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1995
• Tom: 36
• Numer: 2
• Opis fizyczny: p.111-118,fig.

Twórcy

autor

Sliwinska E

• University of Technology and Agriculture, Kaliskiego 7, 85-796 Bydgoszcz, Poland

autor

Steen P.

Bibliografia

• AKINERDEM F. (1991). Determination of the ploidy level of pure and mixed plant populations of sugar beet (Beta vulgaris L.) by flow cytometry. Plant Breeding 107: 333-337.
• BEAUMONT V.H., WIDHOLM J.M. (1993). Ploidy variation of pronamide-treated maize calli during long term culture. Plant Cell Rep. 12: 648-651.
• BORMOTOV V.E., LOPATINA T.I. (1986). Poliploidiya i polimorfizm rastenii po velichine kletok. Nauka i Technika, Mińsk, 165.
• BROWN S.C., DEVAUX P., MARIE O., BERGOUNIOUX C., PETIT P.X. (1991). Cytométric en flux: Application à l'analyse de la ploïdic chez les végétaux. Le Technoscopc de Biofutur 47: 2-14.
• BUTTERFASS T. (1966). Neue Aspekte der Polyploidieforschung und -züchtung. Mitteilungen aus der Max-Planck-Gesellschaft, Heft 1: 47-58.
• DAMASZ B., ŁUCHNIAK P. (1988). Nuclear DNA endoreplication and plastid index in mesophyll of some dicotyledonous species. Acta Soc. Bot. Pol. 57(3): 303-316.
• FELTZ H. (1953). Untesuchungen an diploiden und polyploiden Zuckerrüben. Z. Pflanzenzücht. 32: 275-300.
• GALBRAITH D.W. (1989). Analysis of higher plants by flow cytometry and cell sorting. Int. Rev. Cytol. 116:165-228.
• GALBRAITH D.W., HARKINS K.R., MADDOX J.M., AYRES N.M., SHARMA D.P., FIROOZABADY E. (1983). Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049-1051.
• HECKER R. J. (1988). Pollen characteristics of diploid and tetraploid sugarbeet. J. Sugar Beet Res. 25/1: 55-62.
• LAAT de A.M.M., GHÖDE W., VOGELZANG M.J.D.C. (1987). Determination of ploidy of single plants and plant populations by flow cytometry. Plant Breeding 99: 303-307.
• ŚLIWIŃSKA E. (1993). Cytometria przepływowa - nowa metoda analizy cyklu komórkowego i genomu roślin wyższych. Hodowla Roślin i Nasiennictwo 1: 10-15.
• UIJTEWAAL B.A. (1987). Ploidy variability in greenhouse cultured and in vitro propagated potato (Solanum tuberosum) monohaploids (2n=x=12) as determined by flow cytometry. Plant Cell Rep. 6: 252-255.