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2006 | 48 | 1 |

Tytuł artykułu

Influence of N,N-bis[3-aminopropylo]dodecyloamine on the ultrastructure of nuclei in Aspergillus niger mycelium and on cell proliferation and mitotic disturbances in Allium cepa L. root meristem

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Hyphae growing for 1 h in the presence of 0.05% and 0.1% N,N-bis(3-aminopropylo)dodecyloamine (APDA) showed malformations in the nuclear structure, including changes in shape, budding, progressive disappearance of the nucleolus and chromatin, and damage of the nuclear envelope. The Allium test revealed that at both concentrations used (0.005%, 0.01%) the tested substance lowered the frequency of dividing cells and induced mitotic abnormalities such as c-metaphases, anaphase-telophase bridges and sticky chromosomes, lagging chromosomes, micronuclei, binucleate cells, budding nuclei and partial extrusion of the nucleolus from the nucleus. These data indicate that APDA has cytotoxic, mitodepressive and turbogenic effects, especially at the higher dose. The results showed that APDA may be safely used as a microbicidal agent to destroy microorganisms developing on experimental material.

Wydawca

-

Rocznik

Tom

48

Numer

1

Opis fizyczny

p.45-52,fig.,ref.

Twórcy

autor
  • University of Lodz, Banacha 12/16, 90-227 Lodz, Poland
autor
autor
autor
autor

Bibliografia

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  • Grant WF. 1994. The present status of higher plant bioassays for the detection of environmental mutagens. Mutation Research 426: 175-183.
  • Hsu TC, Liang JC, and Sataya-Prakash KL. 1986. Cytogenetic assays for mitotic poisons using somatic animal cells. In: Serres FJ [ed.], Chemical mutagens, principles and methods for their detection 10, 155-182. Plenum Press.
  • Kontek R. 1999. Ocena mutageniczności i cytotoksyczności toksyn produkowanych przez sinice tworzące zakwity w Sulejowskim Zbiorniku zaporowym w porównaniu z efektem insektycydu fosforooganicznego dichlorfosu. PhD. dissertation. University of Łódź.
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  • Kuźniak E, Wyrwicka A, Gabara B, Koziróg A, and Skłodowska M, 2006. Effects of N,N-bis(3-aminopropyl}dodecylamine on antioxidant enzyme activities, mitochondria morphology and metabolism in Aspergillus niger. Folia Microbiologica 51: 38-44.
  • Müller WU, and Streffer C. 1994. Micronucleus assays. In: Obe G [ed.], Advances in mutagenesis research 5. Springer-Verlag.
  • Osiecka R. 1993. Cytogenetyczne badania wpływu pestycydów - potencjalnych mutagenów - na komórki merystemów korzeniowych Vicia faba. Acta Universitatis Lodziensis 1-182.
  • Panda BB, and Panda KK. 2002. Genotoxicity and mutagenicity of metals in plants. In: Prasad MNV and Strzałka K [eds.], Physiology and biochemistry of metal toxicity and tolerance in plants, 395-414. Kluwer, Academic Publishers.
  • Pawiroharsono S, Naji B, Bonaly R. Tonetti F, Chasseboeuf C, and Richter JP. 1987. Permeability and membrane sterol distribution in Saccharomyces uvarum and Kluyveromyces bulgaricus grown in presence of polyoxyalkylene glycol-oleic acid condensates. Applied Microbiology and Biotechnology 27: 181-185.
  • Reynolds ES. 1963. The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology 17: 208-212.
  • Scott D. 1990. Clastogenesis in vitro under extreme culture conditions. In: Obe G, and Natarajan AT [eds.], Chromosomal aberrations, basic and applied aspects, 273-286. Springer-Verlag.
  • Stopper H, and Müller SO. 1997. Micronuclei as a biological endpoint for genotoxicity: a minireview. Toxicology In Vitro 11: 661-667.
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  • Wierzbicka M. 1988. Mitotic disturbances induced by low doses of inorganic lead. Caryologia 41: 143-160.

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Bibliografia

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