Charakterystyka elektroforetyczna bialek blony zewnetrznej szczepow Salmonella enteritidis hodowanych w zroznicowanych warunkach oraz ocena ich wlasciwosci antygenowych

• Autorzy: Puchalski A , Kolasa A. , Dec M. , Urban-Chmiel R. , Kowalczyk-Pecka D.

Warianty tytułu

EN

Electrophoretic characterization of outer membrane proteins of Salmonella enteritidis strains grown in diversified conditions and the estimation of their antigenic properties

• Języki publikacji: PL

Abstrakty

EN

The purpose of the study was both to estimate the electrophoretic profiles (SDS-PAGE) of outer membrane proteins among Salmonella Enteritidis strains isolated from humans and poultry, as well as to identify the antigens which can induce the appearance of a specific antibody response among vaccinated animals. The densitometry analysis of electrophoregrams showed that a single protein complex of 21 fractions was distinctive for strains grown in standard conditions. Among these fractions those with 20, 22, 35 and 37 kDa characterized by high optical density were found dominant. A single protein profile with 24 fractions was also characteristic of strains grown in conditions with restricted access to iron ions. A high optical density was a feature of proteins with 19, 35, 37, 55 and 74 kDa. Adding of 200 µM 2,2’-dipirydyl to the culture medium caused the expression of proteins and 78 kDa taking part in obtaining and transporting iron through S. Enteritidis within a mass range of 19, 55, 70, and 74. Densitometry analysis of electrophoregrams obtained from nitrocellulose membranes for proteins from bacterial strains grown in conditions with restricted access to iron ions indicated the presence of specific, intensive reactions towards the antigens of 35 and 55 kDa. As far as proteins obtained from bacterial culture grown with and without the addition of chelator were concerned, distinctive reactions were found towards antigens of 35 and 58 kDa. The main protein present in electrophoregrams of bacteria grown both with and without a supplement of chelator and in immunoblotting reactions was that of 35 kDa, belonging to porins of OmpA type.

Słowa kluczowe

PL

szczepy bakteryjne; bialka blony zewnetrznej; wlasciwosci antygenowe; Salmonella enteritidis; profile elektroforetyczne

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 02
• Opis fizyczny: s.193-196,fot.,bibliogr.

Twórcy

autor

Puchalski A

• Akademia Rolnicza w Lublinie, ul.Akademicka 12, 20-033 Lublin

autor

Kolasa A.

autor

Dec M.

autor

Urban-Chmiel R.

autor

Kowalczyk-Pecka D.

Bibliografia

• 1. Bradford M. M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein due binding. Anal. Biochem. 1976, 72, 248-254.
• 2. Chander H., Majumdar S., Sapru S., Rishi P.: 55 kDa outer-membrane protein from short-chain fatty acids exposed Salmonella enterica serovar Typhi induces apoptosis in macrophages. Antonie van Leeuwenhoek 2006, 89, 317-323.
• 3. Chart H., Rowe B.: Iron restriction and the growth of Salmonella enteritidis. Epidemiol. Infect. 1993, 110, 41-47.
• 4. Fadl A. A., Venkitaanareyanan K. S., Khan M. I.: Identification of Salmonella Enteritidis outer membrane proteins expressed during attachment to human intestinal epithelial cells. J. Appl. Microbiol. 2002, 92, 180-186.
• 5. Głośnicka R., Dziadziuszko H., Kunikowska D., Lis M., Wicha M., Heleski M., Tokarska-Pietrzak E., Strzałkowski L., Bugajak P.: Immunovac - poliwalentna szczepionka przeciw salmonellozom kur. Medycyna Wet. 2005, 61, 1105-1109.
• 6. Guina T., Yi E. C., Wang H., Hackett M., Miller S. I.: A PhoP-regulated outer membrane protease of Salmonella enterica serowar typhimurium promotes resistance to alpha helical antimicrobial peptides. J. Bacteriol. 2000, 182, 4077-4086.
• 7. İçgen B., Gürakan G. C., Özcengiz G.: Characterization of Salmonella Enteritidis isolates of chicken, egg and human origin from Turkey. Food Microbiol. 2002, 19, 375-382.
• 8. İçgen B., Gürakan G. C., Özcengiz G.: Effects of plasmid curing on antibiotic susceptibility, phage type, lipopolysaccharide and outer membrane protein profiles in local Salmonella isolates. Food Microbiol. 2001, 18, 631-635.
• 9. Kingsley R., Rabsch W., Stephens P., Roberts M., Reissbrodt R., Williams P. H.: Iron supplying systems of Salmonella in diagnostics, epidemiology and infection. Immunol. Med. Microbiol. 1995, 11, 257-264.
• 10. Kochan I., Wagner S. K., Wasynczuk J.: Effect of iron on antibacterial immunity in vaccinated of mice. Infect. Immun. 1984, 43, 543-548.
• 11. Kowalczyk-Pecka D., Wernicki A., Puchalski A.: Lekowrażliwość oraz typy bakteriofagowe szczepów Salmonella Enteritidis izolowanych na terenie makroregionu lubelskiego. Przegl. Epidemiol. 2003, 57, 201-209.
• 12. Laemmli U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature 1970, 227, 680-685.
• 13. Lin J., Huang S., Zhang Q.: Outer membrane proteins: key players for bacterial adaptation in host niches. Microbes Infect. 2002, 4, 325-331.
• 14. Mastroeni P., Chabalgoity J. A., Dunstan S. J., Maskell D. J., Dougan G.: Salmonella: immune responsem and vaccines. Vet. J. 2000, 161, 132-164.
• 15. Meenakshi M., Bakshi C. S., Butchaiah G., Bansal M. P., Siddiqui M. Z., Singh V. P.: Adjuvanted outer membrane protein vaccine protects poultry against infection with Salmonella enteritidis. Vet. Res. Commun. 1999, 23, 81-90.
• 16. Morton R. J., Simons K. R., Confer A. W.: Major outer membrane proteins of Pasteurella haemolytica serovars 1-15: comparrison of separation techniques and surface-exposed proteins on selected serovars. Vet. Microbiol. 1996, 51, 319-330.
• 17. Ochoa-Repáraz J., Sesma B., Álvarez M., Renedo M. J., Irache J. M., Gamazo C.: Humoral immune response in hens naturally infected with Salmonella Enteritidis against outer membrane proteins an other surface structural antigens. Vet. Res. 2004, 35, 291-298.
• 18. Polska Norma PN ISO 6579:2003: Mikrobiologia - ogólne zasady metod wykrywania pałeczek Salmonella.
• 19. Poppe C., McFadden K. A., Brouwer A. M., Demczuk W.: Characterization of Salmonella enteritidis strains. Can. J. Vet. Res. 1993, 57, 176-184.
• 20. Przybylska A.: Zatrucia i zakażenia pokarmowe w 2000 roku. Przegl. Epidemiol. 2002, 56, 293-304.
• 21. Rose N., Beaudeau F., Drouin P., Toux J. Y., Rose V., Colin P.: Risk factors for Salmonella enterica subsp. enterica contamination in French broiler-chicken flocks at the end of the rearing period. Preventive Vet. Med. 1999, 39, 265-277.
• 22. Tabaraie B., Sharma B. K., Sharma P. R., Sehgal R., Ganguly N. K.: Evaluation of Salmonella porins as a broad spectrum vaccine candidate. Microb. Immunol. 1994, 38, 553-559.
• 23. Towbin H., Staehelin T., Gordon J.: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 1979, 76, 4350-4354.
• 24. Ustawa z dnia 11 marca 2004 r. O ochronie zdrowia zwierząt oraz zwalczaniu chorób zakaźnych zwierz¹t. Dz. U. Nr 69, poz. 625.
• 25. Wizemann T., Adamou J., Langermann S.: Adhesins as targets for vaccine development. Emerg. Infect. Dis. 1999, 5, 395-403.
• 26. Zhang-Barber L., Turner A. K., Barrow P. A.: Vaccination for control of Salmonella in poultry. Vaccine 1999, 17, 2538-2545.