PL
Zawartość kwasu izocytrynowego w naturalnych sokach owocowych oznaczano skojarzoną metodą enzymatyczno-spektro-fluorymetryczną. Badania wykazały, że zawartość tego kwasu w sokach owocowych jest niska i wynosi ok. 30 mg/dm3.
EN
The enzymatic-spectrofluorymetric method to establish the content of isocitric acid (ICA) in of fruit juices was used. Enzymatic methods and particular enzymatic-spectrophotometry methods are used more and more often in food analysis. They are used to estimate the content of toxic compounds which exist in food. Sensitivity of the method worked out by us is satisfactory for the determination of ICA level in fruit juices (1-52 ng/dm3). These amounts are even a few times smaller than amounts citric acid in fruit juices. The standard curve received is rectilinear from O to 50 |ig ICA and used for the estimation within this range. The results also show a considerable variation in isocitric acid content of the examined natural fruit juices. Isocitric acid content in the analysed samples was low, within 20-46 mg/dm3.