A comparison between the crystal and solution structures of Escherichia coli asparaginase II

• Autorzy: Kozak M. , Jurga S.
• Języki publikacji: EN

Abstrakty

EN

The small angle X-ray scattering (SAXS) pattern of the homotetrameric aspa­raginase II from Escherichia coli was measured in solution in conditions resembling those in which its crystal form was obtained and compared with that calculated from the crystallographic model. The radius of gyration measured by SAXS is about 5% larger and the maximum dimension in the distance distribution function about 12% larger than the corresponding value calculated from the crystal structure. A compari­son of the experimental and calculated distance distribution functions suggests that the overall quaternary structure in the crystal and in solution are similar but that the homotetramer is less compact in solution than in the crystal.

Słowa kluczowe

EN

asparaginase; small angle X-ray scattering; solution structure; Escherichia coli; amidohydrolase; crystal structure

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 2
• Opis fizyczny: p.509-513,fig.

Twórcy

autor

Kozak M.

• Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland

autor

Jurga S.

Bibliografia

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