The construction and characteristics of a BAC library for Cucumis sativus L. 'B10'

• Autorzy: Gutman W , Pawelkowicz M. , Woycicki R. , Piszczek E. , Przybecki Z.
• Języki publikacji: EN

Abstrakty

EN

Cloning using bacterial artificial chromosomes (BACs) can yield high quality genomic libraries, which are used for the physical mapping, identification and isolation of genes, and for gene sequencing. A BAC genomic library was constructed from high molecular weight DNA (HMW DNA) obtained from nuclei of the cucumber (Cucumis sativus L. cv. Borszczagowski; B10 line). The DNA was digested with the HindIII restriction enzyme and ligated into the pCC1BAC vector. The library consists of 34,560 BAC clones with an average insert size of 135 kb, and 12.7x genome coverage. Screening the library for chloroplast and mitochondrial DNA content indicated an exceptionally low 0.26% contamination with chloroplast DNA and 0.3% with mitochondrial DNA.

Słowa kluczowe

EN

Cucumis sativus; artificial chromosome; bacterial chromosome; high molecular weight DNA; pulsed-field gel electrophoresis; chloroplast; mitochondrial DNA

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2008
• Tom: 13
• Numer: 1
• Opis fizyczny: p.74-91,fig.,ref.

Twórcy

autor

Gutman W

• Warsaw Agricultural University, 02-776 Warsaw, Poland

autor

Pawelkowicz M.

autor

Woycicki R.

autor

Piszczek E.

autor

Przybecki Z.

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