Hydrolysis cyclic GMP in rat peritoneal macrophages

• Autorzy: Witwicka H. , Kobialka M. , Gorczyca W.A.
• Języki publikacji: EN

Abstrakty

EN

Intact rat peritoneal macrophages (rPM) treated with 3-isobutyl-1-methylxanthine (IBMX), an inhibitor of phosphodiesterases (PDEs), accumulated more cGMP than untreated cells. A PDE activity toward [3H]cGMP was detected in the soluble and particulate fractions of rPM. The hydrolysis of cGMP was Ca2+ /calmodulin-independent but increased in the presence of cGMP excess. Similar results were obtained when3 [ 3H]cAMP was used as a substrate. The hydrolytic activity towards both nucleotides was inhibited in the presence of IBMX. Therefore, the PDEs of families 2,5,10 and 11 are potential candidates for cGMP hydrolysis in the rPM. They may not only regulate the cGMP level in a feedback-controlled way but also link cGMP-dependent pathways with those regulated by cAMP.

Słowa kluczowe

EN

phosphodiesterase; macrophage; signal transduction; cyclic nucleotide; 3-isobutyl-1-methylxanthine; rat; hydrolysis

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 4
• Opis fizyczny: p.891-897,fig.

Twórcy

autor

Witwicka H.

• Polish Academy of Sciences, R.Weigla 12, 53-114 Wroclaw, Poland

autor

Kobialka M.

autor

Gorczyca W.A.

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