Preliminary observations on the effect of calpastatin gene [CAST] polymorphism on carcass traits in pigs

• Autorzy: Kuryl J , Kapelanski W. , Pierzchala M. , Grajewska S. , Bocian M.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to characterize the polymorphism of calpastatin (CAST) gene identified with three restriction enzymes (HinfI, MspI, RsaI) in several pig breeds and lines bred in Poland and to evaluate the relation between the CAST genotypes and carcass traits. The analyses covered a total of 294 fatteners of Polish Landrace (PL), Pietrain (P), Zlotnicka Spotted (ZS), Torhyb [P × (Polish Large White × PL)] and Stamboek (Dutch Large White × Dutch Landrace). P pigs appeared to be monomorphic at each of loci considered, i.e. CAST/HinfI, CAST/MspI and CAST/RsaI, whereas all three genotypes at these loci were observed only in ZS and Stamboek pigs. An association between genotypes at locus CAST and carcass traits was analysed on 39 Stamboek castrated males free of RYR1T gene. Genotypes DD at locus CAST/MspI and EF at locus CAST/RsaI proved less fatty (thinnest backfat and lower weight of backfat with skin in loin) than two remaining genotypes at each of these loci. Moreover, genotypes DD at locus CAST/MspI and EE at locus CAST/RsaI were the most advantageous for eye-muscle area when compared to the remaining genotypes at these loci. It is concluded that CAST gene may be considered as a candidate gene for pig carcass quality. However,further studies are needed on more numerous animal material covering breeds (e.g. Large White) with all possible genotypes at loci considered in this report.

PL

Celem badań była charakterystyka polimorfizmu genu kalpastatyny (CAST) identyfikowanego enzymami restrykcyjnymi HinfI, MspI i RsaI oraz ocena wpływu tego polimorfizmu na cechy jakości tuszy. Polimorfizm genu przebadano u 294 tuczników następujących ras i linii: pbz, pietrain, złotnicka pstra, Torhyb [pietrain × (wbp × pbz)] i Stamboek (holenderska wielka biała × holenderska zwisłoucha) – odpowiednio 50, 30, 30, 145 i 39 zwierząt. Świnie pietrain okazały się monomorficzne względem wszystkich trzech miejsc restrykcyjnych identyfikowanych w genie CAST, natomiast wszystkie możliwe genotypy CAST zidentyfikowano u świń złotnickich pstrych i w linii Stamboek. Istotność zależności między genotypem CAST a cechami jakości tuszy przeanalizowano na 39 tuczonych kastrowanych knurkach Stamboek wolnych od genu podatności na stress (genotyp CC). Pozwoliło to pominąć w analizie statystycznej wpływ płci i genotype RYR1 na cechy tuszy oraz porównać między sobą wszystkie genotypy CAST, które występowały obok siebie właśnie w tej linii. Stwierdzono, że najcieńszą słoninę w niektórych punktach pomiaru oraz najmniejszą zawartość słoniny ze skórą w polędwicy wykazały zwierzęta o genotypie DD w locus CAST/MspI i EF w locus CAST/RsaI. Z kolei genotypy DD w locus CAST/MspI i FF w locus CAST/RsaI były najbardziej korzystne pod względem powierzchni oka polędwicy. Podobną zależność odnotowano dla masy mięsa w półtuszy. Autorzy wnioskują, że gen CAST można by uważać za gen kandydujący o istotnym oddziaływaniu na cechy tuszy. Konieczna jest jednak kontynuacja badań na liczniejszym materiale. Najbardziej dogodną rasą do tych badań byłaby wielka biała. Wynika to z danych pochodzących z piśmiennictwa poświęconego tej rasie, jak również z przedstawionego w niniejszej pracy odpowiednio licznego udziału genotypów CAST.

Słowa kluczowe

EN

pig; carcass trait; carcass quality; calpastatin gene; gene polymorphism; candidate gene; animal breeding; Polish Large White breed; Polish Landrace breed; Pietrain breed; Zlotnicka Spotted breed

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2003
• Tom: 21
• Numer: 2
• Opis fizyczny: p.87-95

Twórcy

autor

Kuryl J

• Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Kapelanski W.

autor

Pierzchala M.

autor

Grajewska S.

autor

Bocian M.

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