Overexpression of the yeast HAM1 gene prevents 6-N-hydroxylaminopurine mutagenesis in Escherichia coli

• Autorzy: Kozmin S. G. , Leroy P. , Pavlov Y.I.
• Języki publikacji: EN

Abstrakty

EN

The base analogue 6-N-hydroxylaminopurine (HAP) is a potent mutagen in a variety of prokaryotic and eukaryotic organisms. Mutations in the yeast ham1 gene render the cells hypersensitive to the mutagenic effect of HAP. We have found that this gene has homologues in a variety of organisms from bacteria to man. We have overexpressed yeast Ham1p in E. coli. We demonstrate that under conditions when this protein constitutes approximately 30% of cellular protein, the host strain is protected both from toxic and mutapgenic effects of HAP. This result indicates that sole Ham1p activity might be sufficient for destruction of HAP or its metabolites in bacterial cells.

Słowa kluczowe

EN

yeast; nucleic acid; cellular metabolism; mutagenesis; bacteria; Escherichia coli; toxic effect; 6-N-hydroxylaminopurine

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 3
• Opis fizyczny: p.645-652,fig.

Twórcy

autor

Kozmin S. G.

• Sankt-Petersburg State University, Universitetskaya emb.7-9, Sankt-Petersburg, 199034 Russia

autor

Leroy P.

autor

Pavlov Y.I.

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