Production and characterization of amphiploids of Aegilops kotschyi and Ae. biuncialis with Secale cereale, and of backcross hybrids of Ae. biuncialis x S. cereale amphiploids with 2x and 4x S. cereale

• Autorzy: Wojciechowska B , Pudelska H
• Języki publikacji: EN

Abstrakty

EN

Amphiploids (2n = 6x = 42) of Ae. kotschyi and Ae. biuncialis with self-compatible S. cereale were produced from F₁ sterile hybrids (2n = 3x = 21) through colchicine treatment and callus tissue regeneration. The amphiploids resembled the F₁ plants in overall morphology, but were larger in all respects and self-fertile. The spikelets consisted mostly of 3 well-developed florets. Selfed seeds were obtained from some colchicine-doubled sectors and callus regenerates. Most of the produced seeds were well developed. Backcrosses between amphiploids and rye (2x and 4x) resulted in obtaining (Ae. biuncialis x S. cereale amphiploid) x S. cereale hybrids via embryo culture. The BC₁ plants (2n = 4x = 28 and 2n = 5x = 35, respectively) were phenotypically intermediate between the parents and vigorous in vegetative growth. Some seeds were obtained only from the 35-chromosome BC₁ hybrids.

Słowa kluczowe

EN

Secale cereale; amphiploid; Aegilops kotschyi; embryo culture; fertility; plant genetics; backcross hybrid; Aegilops biuncialis

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2005
• Tom: 46
• Numer: 2
• Opis fizyczny: p.157-161,fig.,ref.

Twórcy

autor

Wojciechowska B

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Pudelska H

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

Bibliografia

• Fedak G, 1984. Cytogenetics of tissue culture regenerated hybrids of Triticum tauschii x Secale cereale. Can J Genet Cytol 26: 382-386.
• Jiang J, Friebe B, Gill BS, 1994. Recent advances in alien gene transfer in wheat. Euphytica 73: 199-212.
• Kalinowski A, Winiarczyk K, Wojciechowska B, 2001. Pollen proteins after two-dimensional gel electrophoresis and pollen morphology of the amphiploids Aegilops kotschyi and Ae. variabilis with Secale cereale. Sex Plant Reprod 14: 153-161.
• Kalinowski A, Wojciechowska B, Borzyszkowska E, 2003. Isozymes in Aegilops kotschyi and Ae. biuncialis x Secale cereale hybrids and Ae. kotschyi x S. cereale amphiploids in relation to their parents. J Appl Genet 44: 35-43.
• Kalinowski A, Wojciechowska B, 2003. Pollen and leaf proteins after 2-D electrophoresis of the Aegilops geniculata x Secale cereale hybrids, amphiploids and parental forms. Euphytica 133: 202-207.
• Kalinowski A, Wojciechowska B, 2004. Two-dimensional leaf isozyme patterns of Aegilops kotschyi x Secale cereale amphiploids. Acta Physiol Plant 26: 75-84
• Nakamura C, Keller WA, Fedak G, 1981. In vitro propagation and chromosome doubling of Triticum crassum x Hordeum vulgare intergeneric hybrids. Theor Appl Genet 60: 89-96.
• Sass JE, 1964. Botanical microtechnique. Iowa State Univ Press, Ames, Iowa.
• Sechnyak AI, Simonenko VK, Makhnovskaya MI, 1992. The culture in vitro of calluses from immature inflorescences of Aegilops-rye hybrids: fertility and cytogenetics of the regenerants. Tsitologiya i Genet 26: 48-52.
• Simonenko VK, Motsny II, Sechnyak AI, Kulbida MP, 1998. The use of wheat-alien and Aegilops-rye amphiploids for introgression of genetic material to wheat. Euphytica 100: 313-321.
• Wojciechowska B, Pudelska H, 1992. Intergeneric hybrids of Hordeum vulgare L. x Secale cereale L. regenerated from embryo callus culture. Genet Pol 33:87-96.
• Wojciechowska B, Pudelska H, 1999. Production, morphology and fertility of the amphiploids Aegilops variabilis x Secale cereale and Ae. kotschyi x S. cereale. Cereal Res Commun 27: 79-82.
• Wojciechowska B, Pudelska H, 2002a. Production and morphology of the hybrids Aegilops kotschyi x Secale cereale and Ae. biuncialis x S. cereale. J Appl Genet 43: 279-285.
• Wojciechowska B, Pudelska H, 2002b. Hybrids and amphiploids of Aegilops ovata L. with Secale cereale L.: production, morphology and fertility. J Appl Genet 43:415-421.