The transcription reinitiation properties of RNA polymerase III in the absence of transcription factors

• Autorzy: Ferrari R , Dieci G.
• Języki publikacji: EN

Abstrakty

EN

Transcription reinitiation by RNA polymerase (Pol) III proceeds through facilitated recycling, a process by which the terminating Pol III, assisted by the transcription factors TFIIIB and TFIIIC, rapidly reloads onto the same transcription unit. To get further insight into the Pol III transcription mechanism, we analyzed the kinetics of transcription initiation and reinitiation of a simplified in vitro transcription system consisting only of Pol III and template DNA. The data indicates that, in the absence of transcription factors, first-round transcription initiation by Pol III proceeds at a normal rate, while facilitated reinitiation during subsequent cycles is compromised.

Słowa kluczowe

EN

RNA polymerase III; transcription reinitiation; transcription factor; living cell; RNA synthesis

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2008
• Tom: 13
• Numer: 1
• Opis fizyczny: p.112-118,fig.,ref.

Twórcy

autor

Ferrari R

• Universita degli Studi di Parma, Viale G.P.Usberti 23/A, 43100 Parma, Italy

autor

Dieci G.

Bibliografia

• 1. Dieci, G. and Sentenac, A. Detours and shortcuts to transcription reinitiation. Trends. Biochem. Sci. 28 (2003) 202-209.
• 2. Dieci, G., and Sentenac, A. Facilitated recycling pathway for RNA polymerase III. Cell 84 (1996) 245-252.
• 3. Ferrari, R., Rivetti, C., Acker, J. and Dieci, G. Distinct roles of transcription factors TFIIIB and TFIIIC in RNA polymerase III transcription reinitiation. Proc. Natl. Acad. Sci. USA 101 (2004) 13442-13447.
• 4. Landrieux, E., Alic, N., Ducrot, C., Acker, J., Riva, M. and Carles, C. A subcomplex of RNA polymerase III subunits involved in transcription termination and reinitiation. EMBO J. 25 (2006) 118-128.
• 5. Dieci, G., Percudani, R., Giuliodori, S., Bottarelli, L. and Ottonello, S. TFIIIC-independent in vitro transcription of yeast tRNA genes. J. Mol. Biol. 299 (2000) 601-613.
• 6. Huet, J., Manaud, N., Dieci, G., Peyroche, G., Conesa, C., Lefebvre, O., Ruet, A., Riva, M. and Sentenac, A. RNA polymerase III and class III transcription factors from Saccharomyces cerevisiae. Methods Enzymol. 273 (1996) 249-267.
• 7. Bardeleben, C., Kassavetis, G.A. and Geiduschek, E.P. Encounters of Saccharomyces cerevisiae RNA polymerase III with its transcription factors during RNA chain elongation. J. Mol. Biol. 235 (1994) 1193-1205.
• 8. Campbell, F.E., Jr., and Setzer, D.R. Transcription termination by RNA polymerase III: uncoupling of polymerase release from termination signal recognition. Mol. Cell. Biol. 12 (1992) 2260-2272.
• 9. Kassavetis, G. A., Riggs, D.L., Negri, R., Nguyen, L.H. and Geiduschek, E.P. Transcription factor IIIB generates extended DNA interactions in RNA polymerase transcription complexes on tRNA genes. Mol. Cell. Biol. 9 (1989) 2551-2566.
• 10. Braglia, P., Dugas, S.L., Donze, D. and Dieci, G. Requirement of Nhp6 proteins for transcription of a subset of tRNA genes and heterochromatin barrier function in Saccharomyces cerevisiae. Mol. Cell. Biol. 27 (2007) 1545-1557.