Msp18, the repetitive sequence specifically interacting with nuclear matrix of rat testis cells

• Autorzy: Rzeszowska-Wolny J. , Rogolinski J.
• Języki publikacji: EN

Abstrakty

The nuclear matrix bound DNA fraction of rat testis showed enrichment in repetitive sequences found in the 450 bp band after gel electrophoresis of the Mspl digested rat DNA. DNA fragments isolated from this band were cloned. DNA of the clone pMspI8 showed homology to some representatives of rat LINE sequence family, and complcxcd in vitro more efficiently with testes nuclear matrix proteins than with yeast ARS1 sequence containing the matrix association region (MAR) or DNA from an other clone, MspI19. Western blot analysis showed that MspI8 sequence interacts with testes matrix protein of about 120 kDa.

Słowa kluczowe

EN

cell; DNA interaction; protein; nuclear matrix; rat repetitive sequence

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1994
• Tom: 41
• Numer: 4
• Opis fizyczny: p.459-466,fig.

Twórcy

autor

Rzeszowska-Wolny J.

• Institute of Oncology, Wybrzeze Armii Krajowej 15, 44-100 Gliwice, Poland

autor

Rogolinski J.

Bibliografia

• 1. Gasser, S.M., Amati, B.B., Cardenas, M.E. & Hofmann, J.F.-X. (1989) Studies on scaffold attachment sites and Iheir relation to genome function. Int. Rev. Cytol. 119,57-96.
• 2. Fey, E.G. & Penman, S. (1988) Nuclear matrix proteins reflect cell type of origin in cultured human cells. Pwc. Natl. Acad. Sci. U.S.A. 85, 121-125.
• 3. Sun, J.-M., Hendzel, M.J. & Davie, J.R. (1992) Nuclear matrix proteins bind very tightly to specific regions of the chicken histone H5 gene. Biochem. Cell Biol. 70, 822-829.
• 4. Stephanova, E., Stancheva, R. & Avramova, Z. (1993) Binding of sequences from the 5' and 3' -nontranscribed spacers of the rat rDNA locus to the nucleolar matrix. Chromosoma 102, 287-295.
• 5. Boulikas, T. (1992) Homeotic protein binding sites, origins of replication, and nuclear matrix anchorage sites share the ATTA and ATITA motifs. J. Cell. Biochem. 50,111-123.
• 6. Izaurralde, E., Kas, E. & Laemmli, U.K. (1989) Highly preferential nucleation of histone HI assembly on scaffold-associated regions. /. Mol. Biol. 210,573-585.
• 7. von Kries, J.P., Buck, F. & Stratling, W.H. (1994) Chicken MAR binding protein pl20 is identical to human heterogeneous nuclear ribonucleo- protein (hnRNP) U. Nucleic Acids Res. 22, 1215-1220.
• 8. Ivanchenko, M. & Avramova, Z. (1992) Interaction of MAR-sequences with nuclear matrix proteins. J. Cell. Biochem. 50,19Q-200.
• 9. Dickinson, L.A., Joh, T., Kohwi, Y. & Kohwi- -Shigematsu, T. (1992) A tissue-specific MAR/SAR DNA binding protein with unusual binding site recognition. Cell 70, 631-645.
• 10. Luderus, M.E.E., de Graaf, A., Mattia, E., den Blaauwen, J.L., Grande, M.A., de Jong, L. & vanDriel, R. (1992) Binding of matrix attachment regions to lamin Bi. Cell 70,949-959.
• 11. Tsutsui, K., Tsutsui, K., Okada, S., Watarai, S., Seki, S., Yasuda, T. & Shohmori, T. (1993) Identification and characterization of a nuclear scaffold protein that binds matrix attachment region DNA. /. Biol Chan. 268,12886-12894.
• 12. Ward, W.S., Partin, A.W. & Coffey, D.S. (1989) DNA loop domains in mammalian sperma­tozoa. Chwmosoim 98,153-159.
• 13. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular cloning. A laboratory manual; 2nd edv Cold Spring Harbor Laboratory Press.
• 14. Cockerill, P.N. & Garrard, W.T. (1986) Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enlianccr in a region containing topoiso- me rase II sites. Cell 44,273-282.
• 15. Wu, F.K., Garcia,J.A., Harrich, D. & Gaynor, R.B. (1988) Purification of the human immuno­deficiency virus type 1 enhancer and TAR binding proteins EBP-1 and UBP-1. EMBO J. 7, 2117-2129.
• 16. Georgiev, G.P., Vassetzky, Y.S., Luchnik, A.N., Chernokhvostov, V.V. & Razin, S.V. (1991) Nuclear skeleton DNA domains control of replication and transcription. Eur. /. Biochem. 200,613-624.
• 17. Turpaev, K.T. & Vassetzky, Y.S. (1990) NucIcar protein factors binding specific DNA sequences. Genetika 26,804-816.
• 18. Farache, G., Razin, S.V., Rzeszowska-Wolny, J., Moreau, J., RecillasTarga, F. & Scherrer, K. (1990) Mapping of structural and transcription related matrix attachment sites in the a globin gene domain of avian crythroblasts and erythrocytes. Mol. Cell. Biol. 10,5349-5358.
• 19. Small, D., Nelkin, B. & Vogelstein, B. (1982) Nonrandom distribution of repeated DNA sequences with respect to supercoiled loops and the nuclear matrix. Proc. Natl. Acad. Sci. U.S.A. 79,5911-5915.
• 20. Neuer-Nitsche, B., Lu, X. & Werner, D. (1988) Functional role of highly repetitive DNA sequence in anchorage of the mouse genome. Nucleic Acids Res. 16,8351-8360.
• 21. Romig, H., Fackelmayer, F.O., Renz, A., Ramsperger, U. & Richter, A. (1992) Cliaracte- rization of SAF-A, a novel nuclear DNA binding protein from HeLa cells with high affinity for nuclear matrix/scaffold attachment DNA elements. EMBO J. 11,3431-3440.
• 22. Molecular Biology of the Male Reproductive System (1993) (de Kretser, D., ed.) Academic Press Inc., New York.