Kinobeon A, purified from cultured safflower cells, is a novel and potent singlet oxygen quencher

• Autorzy: Kambayashi Y. , Takekoshi S. , Nakano M. , Shibamori M. , Hitomi Y. , Ogino K.
• Języki publikacji: EN

Abstrakty

EN

We recently reported that kinobeon A, produced from safflower cells, suppressed the free radical-induced damage of cell and microsomal membranes. In the present study, we investigated whether kinobeon A quenches singlet oxygen, another important active oxygen species. Kinobeon A inhibited the singlet oxygen-induced oxidation of squalene. The second-order rate constant between singlet oxygen and kinobeon A was 1.15 × 1010 M-1s-1 in methanol containing 10% dimethyl sulfoxide at 37°C. Those of α-tocopherol and β-carotene, which are known potent singlet oxygen quenchers, were 4.45 × 108 M-1s-1 and 1.26 × 1010 M-1s-1, respectively. When kinobeon A was incubated with a thermolytic singlet oxygen generator, its concentration decreased. However, this change was extremely small compared to the amount of singlet oxygen formed and the inhibitory effect of kinobeon A on squalene oxidation by singlet oxygen. In conclusion, kinobeon A was a strong singlet oxygen quencher. It reacted chemically with singlet oxygen, but it was physical quenching that was mainly responsible for the elimination of singlet oxygen by kinobeon A. Kinobeon A is expected to have a preventive effect on singlet oxygen-related diseases of the skin or eyes.

Słowa kluczowe

EN

microsomal membrane; quencher; active oxygen species; kinobeon A; cell; cultured cell; antioxidant; safflower; endoperoxide

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2005
• Tom: 52
• Numer: 4
• Opis fizyczny: p.903-907,fig.,ref.

Twórcy

autor

Kambayashi Y.

• Kanazawa University, Ishikawa, Japan

autor

Takekoshi S.

autor

Nakano M.

autor

Shibamori M.

autor

Hitomi Y.

autor

Ogino K.

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