Kinetics of inactivation of glutamate decarboxylase by cysteine-specific reagents

• Autorzy: McCormick S. J. , Tunnicliff G.
• Języki publikacji: EN

Abstrakty

EN

Mer cu ric chlo ride, p-chloromercuribenzoate and 5,5-dithiobis(2-nitrobenzoic acid) irreversibly inhib ited the activityof Escherichiacoliglu ta matedecarboxyl ase. Their sec ond or der rate con stants for in ac ti va tion are 0.463m M-1min-1, 0.034 uM-1min-1, 0.018 uM-1min-1, respectively. The characteristics of the inhibition by the three thiol-group re agents sup ports the idea that cysteinyl res i dues at the bind ing sites for the co factor and/or the substrate are important for enzyme activity in E. coli.

Słowa kluczowe

EN

sulphydryl group; inactivation; glutamate decarboxylase; p-chloromercuribenzoate; kinetics; Escherichia coli; gamma-aminobutyric acid; mercuric chloride

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 2
• Opis fizyczny: p.573-578,fig.

Twórcy

autor

McCormick S. J.

• Indiana University School of Medicine, 8600 University Boulevard, Evansville, IN 47712, USA

autor

Tunnicliff G.

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